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  • pKANNIBAL

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: --
    載體名稱: pKANNIBAL
    質粒類型: 植物表達載體;雙表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: CaMV 35S
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 6063 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 卡那霉素
    篩選標記: --
    備注: --
    產品目錄號: --
    穩定性: --
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pKANNIBAL Map

    載體簡介

    載體序列

    LOCUS       Exported                6063 bp ds-DNA     circular SYN 25-AUG-2017
    DEFINITION  Vector for silencing genes in plants using intron-containing hairpin
                RNA (hpRNA).
    ACCESSION   AJ311873
    VERSION     .
    KEYWORDS    pKANNIBAL
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 6063)
      AUTHORS   Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding
                PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, 
                Green AG, Waterhouse PM.
      TITLE     Construct design for efficient, effective and high-throughput gene 
                silencing in plants.
      JOURNAL   Plant J. 2001;27:581-90.
      PUBMED    11576441
    REFERENCE   2  (bases 1 to 6063)
      AUTHORS   .
      TITLE     Direct Submission
      JOURNAL   Exported Friday, August 25, 2017 from SnapGene Viewer 3.2.1
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..6063
                         /organism="synthetic DNA construct"
                         /lab_host="Plant Cells"
                         /mol_type="other DNA"
         rep_origin      complement(215..643)
                         /direction=LEFT
                         /note="f1 ori"
                         /note="f1 bacteriophage origin of replication; arrow 
                         indicates direction of (+) strand synthesis"
         promoter        670..774
                         /gene="bla"
                         /note="AmpR promoter"
         CDS             1075..1869
                         /codon_start=1
                         /gene="aphA-3"
                         /product="aminoglycoside phosphotransferase"
                         /note="KanR"
                         /note="confers resistance to kanamycin"
                         /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                         TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                         EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                         PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                         FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
         rep_origin      2045..2633
                         /direction=RIGHT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         promoter        4102..4447
                         /note="CaMV 35S promoter"
                         /note="strong constitutive promoter from cauliflower mosaic
                         virus"
         misc_feature    4449..4467
                         /note="MCS 1"
                         /note="multiple cloning site 1"
         intron          4475..5241
                         /gene="pdk"
                         /number="3"
                         /note="PDK intron"
                         /note="pyruvate orthophosphate dikinase intron from 
                         Flaveria trinervia"
         misc_feature    5261..5284
                         /note="MCS 2"
                         /note="multiple cloning site 2"
         terminator      5288..5994
                         /note="OCS terminator"
                         /note="octopine synthase terminator"
    ORIGIN
            1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
           61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
          121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat
          181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
          241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
          301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
          361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
          421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
          481 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
          541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
          601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
          661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
          721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
          781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
          841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
          901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
          961 cgttttccaa tgatgagcac tttttgcaag gaacagtgaa ttggagttcg tcttgttata
         1021 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
         1081 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
         1141 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
         1201 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
         1261 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
         1321 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
         1381 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
         1441 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
         1501 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
         1561 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
         1621 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
         1681 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
         1741 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
         1801 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
         1861 ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga
         1921 gcgtcagacc ccgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
         1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
         2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
         2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
         2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
         2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
         2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
         2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
         2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
         2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
         2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
         2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
         2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
         2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
         2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
         2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
         2881 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
         2941 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
         3001 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgaattt ggccaagtcg
         3061 gcctctaata cgactcacta tagggagctc gtcgagcggc cgctcgacga attaattcca
         3121 atcccacaaa aatctgagct taacagcaca gttgctcctc tcagagcaga atcgggtatt
         3181 caacaccctc atatcaacta ctacgttgtg tataacggtc cacatgccgg tatatacgat
         3241 gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt
         3301 gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca gcaaacagac
         3361 aggttgaact tcatccccaa aggagaagct caactcaagc ccaagagctt tgctaaggcc
         3421 ctaacaagcc caccaaagca aaaagcccac tggctcacgc taggaaccaa aaggcccagc
         3481 agtgatccag ccccaaaaga gatctccttt gccccggaga ttacaatgga cgatttcctc
         3541 tatctttacg atctaggaag gaagttcgaa ggtgaaggtg acgacactat gttcaccact
         3601 gataatgaga aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
         3661 gaggcctacg cagcaggtct catcaagacg atctacccga gtaacaatct ccaggagatc
         3721 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ttgcatcaag
         3781 aacacagaga aagacatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
         3841 ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttcct
         3901 actgaatcta aggccatgca tggagtctaa gattcaaatc gaggatctaa cagaactcgc
         3961 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
         4021 cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt
         4081 ctcagaagac caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct
         4141 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
         4201 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga
         4261 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
         4321 aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
         4381 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
         4441 gaggacacgc tcgaggaatt cggtacccca attggtaagg aaataattat tttctttttt
         4501 ccttttagta taaaatagtt aagtgatgtt aattagtatg attataataa tatagttgtt
         4561 ataattgtga aaaaataatt tataaatata ttgtttacat aaacaacata gtaatgtaaa
         4621 aaaatatgac aagtgatgtg taagacgaag aagataaaag ttgagagtaa gtatattatt
         4681 tttaatgaat ttgatcgaac atgtaagatg atatactagc attaatattt gttttaatca
         4741 taatagtaat tctagctggt ttgatgaatt aaatatcaat gataaaatac tatagtaaaa
         4801 ataagaataa ataaattaaa ataatatttt tttatgatta atagtttatt atataattaa
         4861 atatctatac cattactaaa tattttagtt taaaagttaa taaatatttt gttagaaatt
         4921 ccaatctgct tgtaatttat caataaacaa aatattaaat aacaagctaa agtaacaaat
         4981 aatatcaaac taatagaaac agtaatctaa tgtaacaaaa cataatctaa tgctaatata
         5041 acaaagcgca agatctatca ttttatatag tattattttc aatcaacatt cttattaatt
         5101 tctaaataat acttgtagtt ttattaactt ctaaatggat tgactattaa ttaaatgaat
         5161 tagtcgaaca tgaataaaca aggtaacatg atagatcatg tcattgtgtt atcattgatc
         5221 ttacatttgg attgattaca gttgggaaat tgggttcgaa atcgataagc ttggatcctc
         5281 tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa
         5341 ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt
         5401 tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc
         5461 cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata
         5521 tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt
         5581 gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag
         5641 actgattaca taaatcttat tcaaatttca aaaggcccca ggggctagta tctacgacac
         5701 accgagcggc gaactaataa cgttcactga agggaactcc ggttccccgc cggcgcgcat
         5761 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
         5821 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
         5881 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
         5941 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggacctg
         6001 caggcatgca agctagctta ctagtgatgc atattctata gtgtcaccta aatctgcggc
         6061 cgc
    //
    
    

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