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  • pEarleyGate304

    價格:6500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: CAMBIA
    載體名稱: pEarleyGate304; pEarleyGate 304
    質粒類型: Gateway載體;植物載體;農桿菌雙元表達載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: Gateway
    啟動子:
    載體大小: 10379 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: AcV5 tag (C ter)
    載體抗性: 卡那霉素、氯霉素(僅空載體)
    篩選標記: 草丁膦(磷絲菌素)phosphinothricin、
    克隆菌株: DB3.1菌株
    宿主細胞(系): 植物細胞、農桿菌
    備注: --
    產品目錄號: --
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pEarleyGate304載體圖譜



    載體簡介

    載體序列

    LOCUS       pEarleyGate 304        10379 bp ds-DNA     circular SYN 26-JUL-2016
    DEFINITION  Gateway(R)-compatible promoterless plant transformation vector with 
                a C-terminal AcV5 tag.
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 10379)
      AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
      TITLE     Gateway-compatible vectors for plant functional genomics and 
                proteomics.
      JOURNAL   Plant J. 2006;45:616-29.
      PUBMED    16441352
    REFERENCE   2  (bases 1 to 10379)
      AUTHORS   Pikaard Lab
      TITLE     Direct Submission
      JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
                NTI(R) format
                http://www.snapgene.com
    COMMENT     The sequence was corrected by inserting a G at position 6487.
    COMMENT     VNTDATE|660096000|
    COMMENT     VNTDBDATE|776390400|
    COMMENT     VNTNAME|pEarleyGate 304|
    COMMENT     VNTAUTHORNAME|Pikaard Lab|
    FEATURES             Location/Qualifiers
         misc_feature    3..27
                         /label=LB T-DNA repeat
                         /note="left border repeat from nopaline C58 T-DNA"
         terminator      113..365
                         /label=MAS terminator
                         /note="mannopine synthase terminator"
         CDS             complement(375..926)
                         /codon_start=1
                         /gene="Streptomyces hygroscopicus bar"
                         /product="phosphinothricin acetyltransferase草丁膦(磷絲菌素) 
                         乙酰轉移酶"
                         /label=BlpR
                         /note="confers resistance to bialophos or phosphinothricin"
         promoter        complement(932..1312)
                         /label=MAS promoter
                         /note="mannopine synthase promoter (Velten et al., 1984)"
         protein_bind    1364..1483
                         /gene="mutant version of attR"
                         /label=attR1
                         /bound_moiety="LR Clonase(TM)"
                         /note="recombination site for the Gateway(R) LR reaction"
         promoter        1513..1543
                         /label=lac UV5 promoter
                         /note="E. coli lac promoter with an ""up"" mutation"
         CDS             1597..2256
                         /codon_start=1
                         /gene="cat"
                         /product="chloramphenicol acetyltransferase"
                         /label=CmR
                         /note="confers resistance to chloramphenicol"
         CDS             2598..2903
                         /codon_start=1
                         /gene="ccdB"
                         /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                         /label=ccdB
                         /note="Plasmids containing the ccdB gene cannot be 
                         propagated in standard E. coli strains."
         protein_bind    complement(2944..3068)
                         /gene="mutant version of attR"
                         /label=attR2
                         /bound_moiety="LR Clonase(TM)"
                         /note="recombination site for the Gateway(R) LR reaction"
         CDS             3070..3096
                         /codon_start=1
                         /product="epitope tag from the baculovirus Autographa 
                         californica GP64 envelope fusion protein"
                         /label=AcV5 tag
         terminator      3150..3857
                         /label=OCS terminator
                         /note="octopine synthase terminator"
         misc_feature    4101..4125
                         /label=RB T-DNA repeat
                         /note="right border repeat from nopaline C58 T-DNA"
         CDS             5425..6054
                         /codon_start=1
                         /product="stability protein from Pseudomonas plasmid pVS1"
                         /label=pVS1 StaA
         CDS             6483..7556
                         /codon_start=1
                         /product="replication protein from Pseudomonas plasmid 
                         pVS1"
                         /label=pVS1 RepA
                         /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                         QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                         KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                         GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                         ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                         LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                         LVMRYRNLIEGEASAGS"
         rep_origin      7622..7816
                         /label=pVS1 oriV
                         /note="origin of replication for the Pseudomonas plasmid 
                         pVS1 (Heeb et al., 2000)"
         misc_feature    8160..8300
                         /label=bom
                         /note="basis of mobility region from pBR322"
         rep_origin      complement(8486..9074)
                         /direction=LEFT
                         /label=ori
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(9161..9955)
                         /codon_start=1
                         /gene="aphA-3"
                         /product="aminoglycoside phosphotransferase"
                         /label=KanR
                         /note="confers resistance to kanamycin"
    ORIGIN
            1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
           61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
          121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
          181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
          241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
          301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
          361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
          421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
          481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
          541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
          601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
          661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
          721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
          781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
          841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
          901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
          961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
         1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
         1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
         1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
         1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
         1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
         1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
         1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
         1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
         1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
         1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
         1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
         1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
         1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
         1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
         1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
         1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
         1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
         2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
         2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
         2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
         2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
         2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
         2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
         2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
         2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
         2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
         2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
         2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
         2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
         2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
         2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
         2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
         2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
         3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
         3061 aaagtggtgt cttggaaaga tgcgagcggc tggtcttgac taggtgagtc tagagagtta
         3121 attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga gacgcctatg
         3181 atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta
         3241 gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg
         3301 tatttttatg aataatattc tccgttcaat ttactgattg taccctacta cttatatgta
         3361 caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag
         3421 agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg
         3481 cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc
         3541 ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac
         3601 tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc
         3661 tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact
         3721 tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag
         3781 gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac
         3841 atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
         3901 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
         3961 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
         4021 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
         4081 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
         4141 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
         4201 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
         4261 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
         4321 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
         4381 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
         4441 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
         4501 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
         4561 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
         4621 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
         4681 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
         4741 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
         4801 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
         4861 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
         4921 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
         4981 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
         5041 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
         5101 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
         5161 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
         5221 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
         5281 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
         5341 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
         5401 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
         5461 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
         5521 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
         5581 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
         5641 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
         5701 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
         5761 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
         5821 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
         5881 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
         5941 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
         6001 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
         6061 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
         6121 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
         6181 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
         6241 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
         6301 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
         6361 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
         6421 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
         6481 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
         6541 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
         6601 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
         6661 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
         6721 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
         6781 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
         6841 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
         6901 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
         6961 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
         7021 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
         7081 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
         7141 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
         7201 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
         7261 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
         7321 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
         7381 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
         7441 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
         7501 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
         7561 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
         7621 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
         7681 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
         7741 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
         7801 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
         7861 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
         7921 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
         7981 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
         8041 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
         8101 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
         8161 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
         8221 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
         8281 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
         8341 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
         8401 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
         8461 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
         8521 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
         8581 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
         8641 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
         8701 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
         8761 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
         8821 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
         8881 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
         8941 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
         9001 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
         9061 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
         9121 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
         9181 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
         9241 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
         9301 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
         9361 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
         9421 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
         9481 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
         9541 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
         9601 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
         9661 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
         9721 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
         9781 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
         9841 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
         9901 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
         9961 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
        10021 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
        10081 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
        10141 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
        10201 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
        10261 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
        10321 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
    //
    
    

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