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  • pEarleyGate204

    價格:6500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: CAMBIA
    載體名稱: pEarleyGate204; pEarleyGate 204
    質粒類型: Gateway載體;植物載體;農桿菌雙元表達載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: Gateway
    啟動子: 35S
    載體大小: 11780 bp
    5' 測序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
    3' 測序引物及序列: --
    載體標簽: AcV5 tag (N ter)
    載體抗性: 卡那霉素、氯霉素(僅空載體)
    篩選標記: 草丁膦(磷絲菌素)phosphinothricin、
    克隆菌株: DB3.1菌株
    宿主細胞(系): 植物細胞、農桿菌
    備注: --
    產品目錄號: --
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pEarleyGate204載體圖譜



    載體簡介

    載體序列

    LOCUS       Exported               11780 bp ds-DNA     circular SYN 26-JUL-2016
    DEFINITION  Gateway(R)-compatible plant transformation vector with an N-terminal
                AcV5 tag.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pEarleyGate 204
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 11780)
      AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
      TITLE     Gateway-compatible vectors for plant functional genomics and 
                proteomics.
      JOURNAL   Plant J. 2006;45:616-29.
      PUBMED    16441352
    REFERENCE   2  (bases 1 to 11780)
      AUTHORS   Pikaard Lab
      TITLE     Direct Submission
      JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    COMMENT     The sequence was corrected by inserting a G at position 7888.
    FEATURES             Location/Qualifiers
         source          1..11780
                         /organism="synthetic DNA construct"
                         /lab_host="Plant Cells"
                         /mol_type="other DNA"
         misc_feature    1..25
                         /note="LB T-DNA repeat"
                         /note="left border repeat from nopaline C58 T-DNA"
         terminator      111..363
                         /note="MAS terminator"
                         /note="mannopine synthase terminator"
         CDS             complement(373..924)
                         /codon_start=1
                         /gene="Streptomyces hygroscopicus bar"
                         /product="phosphinothricin acetyltransferase"
                         /note="BlpR"
                         /note="confers resistance to bialophos or phosphinothricin"
                         /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                         WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                         TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                         WQLDFSLPVPPRPVLPVTEI"
         promoter        complement(930..1310)
                         /note="MAS promoter"
                         /note="mannopine synthase promoter (Velten et al., 1984)"
         promoter        2306..2651
                         /note="35S promoter"
                         /note="strong constitutive promoter from cauliflower mosaic
                         virus"
         misc_feature    2681..2735
                         /note="TMV Omega"
                         /note="translational enhancer from the tobacco mosaic virus
                         5'-leader sequence (Gallie et al., 1988)"
         CDS             2737..2739
                         /codon_start=1
                         /product="start codon"
                         /note="ATG"
                         /translation="M"
         CDS             2740..2766
                         /codon_start=1
                         /product="epitope tag from the baculovirus Autographa 
                         californica GP64 envelope fusion protein"
                         /note="AcV5 tag"
                         /translation="SWKDASGWS"
         protein_bind    2770..2889
                         /gene="mutant version of attR"
                         /bound_moiety="LR Clonase(TM)"
                         /note="attR1"
                         /note="recombination site for the Gateway(R) LR reaction"
         promoter        2919..2949
                         /note="lac UV5 promoter"
                         /note="E. coli lac promoter with an ""up"" mutation"
         CDS             3003..3662
                         /codon_start=1
                         /gene="cat"
                         /product="chloramphenicol acetyltransferase"
                         /note="CmR"
                         /note="confers resistance to chloramphenicol"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                         KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                         LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                         DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
         CDS             4004..4309
                         /codon_start=1
                         /gene="ccdB"
                         /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                         /note="ccdB"
                         /note="Plasmids containing the ccdB gene cannot be 
                         propagated in standard E. coli strains."
                         /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                         VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
         protein_bind    complement(4350..4474)
                         /gene="mutant version of attR"
                         /bound_moiety="LR Clonase(TM)"
                         /note="attR2"
                         /note="recombination site for the Gateway(R) LR reaction"
         terminator      4551..5258
                         /note="OCS terminator"
                         /note="octopine synthase terminator"
         misc_feature    5502..5526
                         /note="RB T-DNA repeat"
                         /note="right border repeat from nopaline C58 T-DNA"
         CDS             6826..7455
                         /codon_start=1
                         /product="stability protein from Pseudomonas plasmid pVS1"
                         /note="pVS1 StaA"
                         /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                         DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                         VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                         LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
         CDS             7884..8957
                         /codon_start=1
                         /product="replication protein from Pseudomonas plasmid 
                         pVS1"
                         /note="pVS1 RepA"
                         /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                         QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                         KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                         GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                         ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                         LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                         LVMRYRNLIEGEASAGS"
         rep_origin      9023..9217
                         /note="pVS1 oriV"
                         /note="origin of replication for the Pseudomonas plasmid 
                         pVS1 (Heeb et al., 2000)"
         misc_feature    9561..9701
                         /note="bom"
                         /note="basis of mobility region from pBR322"
         rep_origin      complement(9887..10475)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(10562..11356)
                         /codon_start=1
                         /gene="aphA-3"
                         /product="aminoglycoside phosphotransferase"
                         /note="KanR"
                         /note="confers resistance to kanamycin"
                         /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                         TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                         EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                         PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                         FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
    ORIGIN
            1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
           61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
          121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
          181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
          241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
          301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
          361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
          421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
          481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
          541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
          601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
          661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
          721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
          781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
          841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
          901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
          961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
         1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
         1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
         1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
         1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
         1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
         1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
         1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
         1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
         1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
         1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
         1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
         1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
         1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
         1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
         1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
         1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
         1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
         2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
         2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
         2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
         2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
         2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
         2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
         2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
         2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
         2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
         2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
         2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
         2701 acaacaaaca acattacaat tacatttaca attaccatgt cttggaaaga tgcgagcggc
         2761 tggtctatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
         2821 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
         2881 atccagtcat attggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
         2941 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
         3001 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
         3061 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
         3121 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
         3181 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
         3241 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
         3301 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
         3361 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
         3421 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
         3481 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
         3541 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
         3601 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
         3661 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
         3721 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
         3781 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
         3841 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
         3901 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
         3961 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
         4021 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
         4081 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
         4141 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
         4201 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
         4261 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
         4321 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagc
         4381 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
         4441 acgtttctcg ttcagctttc ttgtacaaag tggtgcctag gtgagtctag agagttaatt
         4501 ctaggtgagt ctagagagtt aattaagacc cgggactagt ccctagagtc ctgctttaat
         4561 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
         4621 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
         4681 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
         4741 gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt
         4801 ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa
         4861 atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct
         4921 tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta
         4981 ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga
         5041 agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc
         5101 acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg
         5161 tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc
         5221 cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctgcaggca tgcaagcttg
         5281 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
         5341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
         5401 cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat
         5461 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
         5521 gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag
         5581 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
         5641 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
         5701 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
         5761 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
         5821 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
         5881 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
         5941 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
         6001 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
         6061 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
         6121 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
         6181 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
         6241 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
         6301 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
         6361 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
         6421 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
         6481 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
         6541 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
         6601 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
         6661 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
         6721 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
         6781 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
         6841 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
         6901 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
         6961 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
         7021 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
         7081 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
         7141 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
         7201 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
         7261 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
         7321 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
         7381 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
         7441 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
         7501 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
         7561 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
         7621 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
         7681 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
         7741 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
         7801 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
         7861 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
         7921 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
         7981 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
         8041 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
         8101 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
         8161 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
         8221 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
         8281 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
         8341 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
         8401 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
         8461 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
         8521 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
         8581 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
         8641 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
         8701 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
         8761 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
         8821 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
         8881 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
         8941 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
         9001 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
         9061 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
         9121 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
         9181 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
         9241 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
         9301 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
         9361 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
         9421 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
         9481 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
         9541 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
         9601 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
         9661 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
         9721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
         9781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
         9841 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
         9901 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
         9961 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
        10021 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
        10081 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
        10141 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
        10201 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
        10261 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
        10321 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
        10381 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
        10441 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
        10501 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
        10561 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
        10621 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
        10681 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
        10741 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
        10801 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
        10861 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
        10921 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
        10981 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
        11041 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
        11101 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
        11161 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
        11221 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
        11281 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
        11341 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
        11401 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
        11461 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
        11521 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
        11581 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
        11641 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
        11701 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
        11761 gggagctgtt ggctggctgg
    //
    
    

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