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  • pEarleyGate203

    價格:6500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: CAMBIA
    載體名稱: pEarleyGate203; pEarleyGate 203
    質粒類型: Gateway載體;植物載體;農桿菌雙元表達載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: Gateway
    啟動子: 35S
    載體大小: 11783 bp
    5' 測序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
    3' 測序引物及序列: --
    載體標簽: Myc(N ter)
    載體抗性: 卡那霉素、氯霉素(僅空載體)
    篩選標記: 草丁膦(磷絲菌素)phosphinothricin、
    克隆菌株: DB3.1菌株
    宿主細胞(系): 植物細胞、農桿菌
    備注: --
    產品目錄號: --
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pEarleyGate203載體圖譜



    載體簡介

    載體序列

    LOCUS       Exported               11783 bp ds-DNA     circular SYN 26-JUL-2016
    DEFINITION  Gateway(R)-compatible plant transformation vector with an N-terminal
                Myc tag.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pEarleyGate 203
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 11783)
      AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
      TITLE     Gateway-compatible vectors for plant functional genomics and 
                proteomics.
      JOURNAL   Plant J. 2006;45:616-29.
      PUBMED    16441352
    REFERENCE   2  (bases 1 to 11783)
      AUTHORS   Pikaard Lab
      TITLE     Direct Submission
      JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    COMMENT     The sequence was corrected by inserting a G at position 7891.
    FEATURES             Location/Qualifiers
         source          1..11783
                         /organism="synthetic DNA construct"
                         /lab_host="Plant Cells"
                         /mol_type="other DNA"
         misc_feature    1..25
                         /note="LB T-DNA repeat"
                         /note="left border repeat from nopaline C58 T-DNA"
         terminator      111..363
                         /note="MAS terminator"
                         /note="mannopine synthase terminator"
         CDS             complement(373..924)
                         /codon_start=1
                         /gene="Streptomyces hygroscopicus bar"
                         /product="phosphinothricin acetyltransferase草丁膦(磷絲菌素) 
                         乙酰轉移酶"
                         /note="BlpR"
                         /note="confers resistance to bialophos or phosphinothricin"
                         /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                         WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                         TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                         WQLDFSLPVPPRPVLPVTEI"
         promoter        complement(930..1310)
                         /note="MAS promoter"
                         /note="mannopine synthase promoter (Velten et al., 1984)"
         promoter        2306..2651
                         /note="CaMV 35S promoter"
                         /note="strong constitutive promoter from cauliflower mosaic
                         virus"
         misc_feature    2681..2735
                         /note="TMV Omega"
                         /note="translational enhancer from the tobacco mosaic virus
                         5'-leader sequence (Gallie et al., 1988)"
         CDS             2737..2739
                         /codon_start=1
                         /product="start codon"
                         /note="ATG"
                         /translation="M"
         CDS             2740..2769
                         /codon_start=1
                         /product="Myc (human c-Myc oncogene) epitope tag"
                         /note="Myc"
                         /translation="EQKLISEEDL"
         protein_bind    2773..2892
                         /gene="mutant version of attR"
                         /bound_moiety="LR Clonase(TM)"
                         /note="attR1"
                         /note="recombination site for the Gateway(R) LR reaction"
         promoter        2922..2952
                         /note="lac UV5 promoter"
                         /note="E. coli lac promoter with an ""up"" mutation"
         CDS             3006..3665
                         /codon_start=1
                         /gene="cat"
                         /product="chloramphenicol acetyltransferase"
                         /note="CmR"
                         /note="confers resistance to chloramphenicol"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                         KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                         LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                         DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
         CDS             4007..4312
                         /codon_start=1
                         /gene="ccdB"
                         /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                         /note="ccdB"
                         /note="Plasmids containing the ccdB gene cannot be 
                         propagated in standard E. coli strains."
                         /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                         VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
         protein_bind    complement(4353..4477)
                         /gene="mutant version of attR"
                         /bound_moiety="LR Clonase(TM)"
                         /note="attR2"
                         /note="recombination site for the Gateway(R) LR reaction"
         terminator      4554..5261
                         /note="OCS terminator"
                         /note="octopine synthase terminator"
         misc_feature    5505..5529
                         /note="RB T-DNA repeat"
                         /note="right border repeat from nopaline C58 T-DNA"
         CDS             6829..7458
                         /codon_start=1
                         /product="stability protein from Pseudomonas plasmid pVS1"
                         /note="pVS1 StaA"
                         /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                         DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                         VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                         LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
         CDS             7887..8960
                         /codon_start=1
                         /product="replication protein from Pseudomonas plasmid 
                         pVS1"
                         /note="pVS1 RepA"
                         /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                         QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                         KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                         GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                         ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                         LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                         LVMRYRNLIEGEASAGS"
         rep_origin      9026..9220
                         /note="pVS1 oriV"
                         /note="origin of replication for the Pseudomonas plasmid 
                         pVS1 (Heeb et al., 2000)"
         misc_feature    9564..9704
                         /note="bom"
                         /note="basis of mobility region from pBR322"
         rep_origin      complement(9890..10478)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(10565..11359)
                         /codon_start=1
                         /gene="aphA-3"
                         /product="aminoglycoside phosphotransferase"
                         /note="KanR"
                         /note="confers resistance to kanamycin"
                         /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                         TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                         EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                         PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                         FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
    ORIGIN
            1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
           61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
          121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
          181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
          241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
          301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
          361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
          421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
          481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
          541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
          601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
          661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
          721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
          781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
          841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
          901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
          961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
         1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
         1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
         1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
         1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
         1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
         1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
         1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
         1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
         1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
         1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
         1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
         1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
         1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
         1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
         1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
         1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
         1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
         2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
         2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
         2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
         2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
         2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
         2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
         2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
         2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
         2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
         2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
         2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
         2701 acaacaaaca acattacaat tacatttaca attaccatgg aacagaaact gatctctgaa
         2761 gaagatctga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
         2821 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
         2881 catatccagt catattggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
         2941 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
         3001 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
         3061 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
         3121 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
         3181 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
         3241 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
         3301 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
         3361 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
         3421 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
         3481 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
         3541 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
         3601 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
         3661 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
         3721 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
         3781 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
         3841 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
         3901 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
         3961 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
         4021 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
         4081 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
         4141 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
         4201 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
         4261 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
         4321 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
         4381 agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
         4441 tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc taggtgagtc tagagagtta
         4501 attctaggtg agtctagaga gttaattaag acccgggact agtccctaga gtcctgcttt
         4561 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
         4621 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
         4681 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
         4741 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
         4801 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
         4861 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
         4921 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
         4981 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
         5041 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
         5101 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
         5161 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
         5221 gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag gcatgcaagc
         5281 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
         5341 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
         5401 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg
         5461 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
         5521 cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa
         5581 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
         5641 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
         5701 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
         5761 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
         5821 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
         5881 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
         5941 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
         6001 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
         6061 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
         6121 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
         6181 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
         6241 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
         6301 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
         6361 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
         6421 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
         6481 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
         6541 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
         6601 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
         6661 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
         6721 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
         6781 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
         6841 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
         6901 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
         6961 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
         7021 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
         7081 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
         7141 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
         7201 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
         7261 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
         7321 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
         7381 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
         7441 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
         7501 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
         7561 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
         7621 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
         7681 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
         7741 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
         7801 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
         7861 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
         7921 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
         7981 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
         8041 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
         8101 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
         8161 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
         8221 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
         8281 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
         8341 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
         8401 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
         8461 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
         8521 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
         8581 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
         8641 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
         8701 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
         8761 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
         8821 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
         8881 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
         8941 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
         9001 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
         9061 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
         9121 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
         9181 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
         9241 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
         9301 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
         9361 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
         9421 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
         9481 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
         9541 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
         9601 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
         9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
         9721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
         9781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
         9841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
         9901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
         9961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
        10021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
        10081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
        10141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
        10201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
        10261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
        10321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
        10381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
        10441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
        10501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
        10561 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
        10621 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
        10681 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
        10741 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
        10801 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
        10861 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
        10921 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
        10981 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
        11041 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
        11101 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
        11161 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
        11221 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
        11281 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
        11341 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
        11401 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
        11461 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
        11521 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
        11581 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
        11641 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
        11701 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
        11761 tcggggagct gttggctggc tgg
    //
    
    

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