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  • pCX-DR

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pCXDR載體基本信息

    出品公司: --
    載體名稱: pCX-DR
    質粒類型: 植物過表達質粒
    高拷貝/低拷貝: --
    終止子: NOS
    啟動子: CaMV35S
    載體大小: 11229 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: mCherry,ccdb
    載體抗性: 卡那霉素
    篩選標記: 潮霉素
    克隆菌株: DB3.1
    宿主細胞(系): --
    備注: --
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    pCXDR載體質粒圖譜和多克隆位點信息

    pCX-DR載體圖譜

    pCXDR載體簡介

    pCXDR載體序列

    LOCUS       pCX-DR               11229 bp ds-DNA     circular SYN 30-OCT-2017
    DEFINITION  synthetic circular DNA
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 11229)
      TITLE     Direct Submission
                http://www.native-nursery.com/
    FEATURES             Location/Qualifiers
         source          1..11229
                         /organism="synthetic DNA construct"
                         /mol_type="other DNA"
         misc_feature    196..655
                         /label=CaMV35S promoter
         regulatory      673..682
                         /regulatory_class="other"
                         /note="vertebrate consensus sequence for strong initiation 
                         of translation (Kozak, 1987)"
         CDS             679..1353
                         /codon_start=1
                         /product="improved tetrameric variant of DsRed fluorescent 
                         protein"
                         /label=DsRed2
                         /note="mammalian codon-optimized"
                         /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                         LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                         ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                         ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                         LFL"
         misc_feature    1354..1410
                         /label=MCS
                         /note="multiple cloning site"
         CDS             complement(1466..1771)
                         /codon_start=1
                         /gene="ccdB"
                         /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                         /label=ccdB
                         /note="Plasmids containing the ccdB gene cannot be 
                         propagated in standard E. coli strains."
                         /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                         VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
         terminator      2279..2531
                         /label=NOS terminator
                         /note="nopaline synthase terminator and poly(A) signal"
         misc_feature    2553..2577
                         /label=RB T-DNA repeat
                         /note="right border repeat from nopaline C58 T-DNA"
         CDS             3876..4505
                         /codon_start=1
                         /product="stability protein from the Pseudomonas plasmid 
                         pVS1 (Heeb et al., 2000)"
                         /label=pVS1 StaA
                         /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                         DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                         VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                         LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
         CDS             4939..6006
                         /codon_start=1
                         /product="replication protein from the Pseudomonas plasmid 
                         pVS1 (Heeb et al., 2000)"
                         /label=pVS1 RepA
                         /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                         AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                         DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                         VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                         ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                         RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                         MRYRNLIEGEASAGS"
         rep_origin      6072..6266
                         /label=pVS1 oriV
                         /note="origin of replication for the Pseudomonas plasmid 
                         pVS1 (Heeb et al., 2000)"
         misc_feature    6610..6750
                         /label=bom
                         /note="basis of mobility region from pBR322"
         rep_origin      complement(6936..7524)
                         /direction=LEFT
                         /label=ori
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(7611..8405)
                         /codon_start=1
                         /gene="aphA-3"
                         /product="aminoglycoside phosphotransferase"
                         /label=KanR
                         /note="confers resistance to kanamycin"
                         /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                         TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                         EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                         PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                         FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
         misc_feature    8830..8854
                         /label=LB T-DNA repeat
                         /note="left border repeat from nopaline C58 T-DNA"
         polyA_signal    8932..9106
                         /label=CaMV poly(A) signal
                         /note="cauliflower mosaic virus polyadenylation signal"
         CDS             complement(9146..10171)
                         /codon_start=1
                         /gene="aph(4)-Ia"
                         /product="aminoglycoside phosphotransferase from E. coli"
                         /label=HygR
                         /note="confers resistance to hygromycin"
                         /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                         YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                         ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                         TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                         GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                         NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                         K"
         promoter        complement(10238..10915)
                         /label=CaMV 35S promoter (enhanced)
                         /note="cauliflower mosaic virus 35S promoter with a 
                         duplicated enhancer region"
    ORIGIN
            1 gaattcgagc tcggtaccca ctggattttg gttttaggaa ttagaaattt tattgataga
           61 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
          121 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
          181 tcgaaattcg agctccaccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
          241 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
          301 ctccgctcat gatccgggga attaattccg atcctatctg tcacttcatc aaaaggacag
          361 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcattc
          421 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
          481 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
          541 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
          601 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca agatcctcta
          661 gcgctaccgg tcgccaccat ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc
          721 aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
          781 ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg
          841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
          901 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
          961 cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc ctccctgcag
         1021 gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc
         1081 gtgatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
         1141 ggcgtgctga agggcgagac ccacaaggcc ctgaagctga aggacggcgg ccactacctg
         1201 gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac
         1261 gtggacgcca agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac
         1321 gagcgcaccg agggccgcca ccacctgttc ctgagatctc gagctcaagc ttcgaattct
         1381 gcagtcgacg gtaccgcggg cccgggatcc ccaatacttg tatgggtcga cctgcagact
         1441 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
         1501 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
         1561 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
         1621 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
         1681 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
         1741 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
         1801 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
         1861 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
         1921 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
         1981 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
         2041 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
         2101 ccatacaagt attggggatc caccggatct agataactga tcataatcag ccataccaca
         2161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
         2221 aaaatgaatg caattgttgt tgttgtgtga attacaggtg accagctcga atttccccga
         2281 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
         2341 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
         2401 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
         2461 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
         2521 gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg ggtaaaccta
         2581 agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg
         2641 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
         2701 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
         2761 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
         2821 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
         2881 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
         2941 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
         3001 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
         3061 accacctagc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
         3121 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
         3181 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
         3241 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
         3301 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
         3361 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
         3421 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
         3481 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
         3541 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
         3601 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
         3661 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
         3721 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
         3781 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
         3841 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
         3901 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
         3961 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
         4021 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
         4081 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
         4141 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
         4201 cttacgacat atgggcaacc gccgacctgg tggagctggt taagcagcgc attgaggtca
         4261 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
         4321 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
         4381 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
         4441 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
         4501 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
         4561 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
         4621 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
         4681 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
         4741 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
         4801 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
         4861 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
         4921 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
         4981 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
         5041 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
         5101 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
         5161 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
         5221 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
         5281 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
         5341 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
         5401 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
         5461 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
         5521 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
         5581 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
         5641 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
         5701 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
         5761 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
         5821 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
         5881 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
         5941 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
         6001 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
         6061 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
         6121 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
         6181 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
         6241 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
         6301 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
         6361 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
         6421 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
         6481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
         6541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
         6601 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
         6661 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
         6721 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
         6781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
         6841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
         6901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
         6961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
         7021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
         7081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
         7141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
         7201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
         7261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
         7321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
         7381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
         7441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
         7501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
         7561 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
         7621 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
         7681 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
         7741 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
         7801 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
         7861 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
         7921 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
         7981 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
         8041 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
         8101 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
         8161 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
         8221 tccttttccc gttccacatc ataggttgtc cctttatacc ggctgtccgt catttttaaa
         8281 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
         8341 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
         8401 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
         8461 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
         8521 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
         8581 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
         8641 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
         8701 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
         8761 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
         8821 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
         8881 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
         8941 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
         9001 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
         9061 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
         9121 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
         9181 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
         9241 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
         9301 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
         9361 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
         9421 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
         9481 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
         9541 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
         9601 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
         9661 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
         9721 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
         9781 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
         9841 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
         9901 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
         9961 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
        10021 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
        10081 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
        10141 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat ctgcgaaagc
        10201 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
        10261 atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct
        10321 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
        10381 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
        10441 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
        10501 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
        10561 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
        10621 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg
        10681 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
        10741 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
        10801 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
        10861 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
        10921 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
        10981 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
        11041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
        11101 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
        11161 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
        11221 catgattac
    //
    
    
    

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