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  • pShuttle

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Agilent
    載體名稱: pShuttle
    GenBank序列號: AF334399
    質粒類型: 腺病毒表達載體,哺乳動物細胞表達載體
    高拷貝/低拷貝: 低拷貝
    啟動子: CMV
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 6568 bp
    5' 測序引物及序列: pShuttle-F: GAAGTGAAATCTGAATAATTTTGTG
    3' 測序引物及序列: pShuttle-R: CAAAACTACATAAGACCCCCAC
    載體標簽: --
    載體抗性: 卡那
    備注: --
    產品目錄號: 240006
    穩定性: /
    組成型: --
    病毒/非病毒: 腺病毒

    載體質粒圖譜和多克隆位點信息

    pShuttle載體圖譜


    pShuttle多克隆位點


    pShuttle載體特征

    載體簡介

    pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.

    載體序列

    LOCUS       pShuttle           6568 bp    DNA     circular SYN 07-FEB-2001
    DEFINITION  AdEasy vector pShuttle, complete sequence.
    ACCESSION   AF334399
    VERSION     AF334399.1  GI:12698892
    KEYWORDS    .
    SOURCE      AdEasy vector pShuttle
      ORGANISM  AdEasy vector pShuttle
                other sequences; artificial sequences; vectors.
    REFERENCE   1  (bases 1 to 6568)
      AUTHORS   Grafsky,A.J. III.
      TITLE     Direct Submission
      JOURNAL   Submitted (08-JAN-2001) Technical Services, Stratagene, 11011 N.
                Torrey Pines Rd, La Jolla, CA 92037, USA
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..6568
                         /organism="AdEasy vector pShuttle"
                         /mol_type="genomic DNA"
                         /db_xref="taxon:148367"
                         /lab_host="Escherichia coli"
                         /label="extrachromosomal
                         used for the cloning of cDNA to be recombined with the
                         pADEasy-1 vector; for infection of, and expression in,
                         various mammalian cell types"
         LTR             4..106
                         /label="left inverted terminal repeat (LITR)"
         misc_signal     197..345
                         /label="encapsidation"
         primer_bind     313..337
                         /label="forward"
         misc_feature    359..418
                         /label="MCS"
         misc_feature    416..2414
                         /label="right arm homology region"
         primer_bind     452..473
                         /label="reverse"
         misc_feature    2682..3583
                         /label="left arm homology region"
         LTR             3584..3686
                         /label="right inverted terminal repeat (RITR)"
         rep_origin      3764..4542
                         /label="pBR322 ori"
         CDS             5289..6083
                         /label="kanamycin resistance protein"
                         /codon_start=1
                         /transl_table=11
                         /product="neomycin phosphotransferase"
                         /protein_id="AAK01721.1"
                         /db_xref="GI:12698893"                     
    ORIGIN      
            1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
           61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
          121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
          181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
          241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
          301 tgttactcat agcgcgtaat actggtaccg cggccgcctc gagtctagag atatcgaatt
          361 caagcttgtc gactcgaaga tctgggcgtg gttaagggtg ggaaagaata tataaggtgg
          421 gggtcttatg tagttttgta tctgttttgc agcagccgcc gccgccatga gcaccaactc
          481 gtttgatgga agcattgtga gctcatattt gacaacgcgc atgcccccat gggccggggt
          541 gcgtcagaat gtgatgggct ccagcattga tggtcgcccc gtcctgcccg caaactctac
          601 taccttgacc tacgagaccg tgtctggaac gccgttggag actgcagcct ccgccgccgc
          661 ttcagccgct gcagccaccg cccgcgggat tgtgactgac tttgctttcc tgagcccgct
          721 tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac aagttgacgg ctcttttggc
          781 acaattggat tctttgaccc gggaacttaa tgtcgtttct cagcagctgt tggatctgcg
          841 ccagcaggtt tctgccctga aggcttcctc ccctcccaat gcggtttaaa acataaataa
          901 aaaaccagac tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt atttaggggt
          961 tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt
         1021 ttccaggacg tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct
         1081 ggggtggagg tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca
         1141 gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc
         1201 caggggcagg cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg
         1261 tggggatatg agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc
         1321 cctccgggga ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa
         1381 tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc
         1441 aagattttcc atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc
         1501 gaagatattt ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc
         1561 catttttaca aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc
         1621 aggggcgtag ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat
         1681 catgtctacc tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga
         1741 agaaagcagg ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc
         1801 tattaccggg tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg
         1861 ggccacttcg ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag
         1921 gcgctcgccg cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag
         1981 accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag
         2041 ctcggtcacc tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg
         2101 cggctttcgc tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc
         2161 cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc
         2221 tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct
         2281 tcgccctgcg cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg
         2341 gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga
         2401 cttttgaggg cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg
         2461 ccgcaggccc cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg
         2521 tcaaaaacca ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc
         2581 cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga ctnnngttta
         2641 aacgaattcn nntataaaat gcaaggtgct gctcaaaaaa tcaggcaaag cctcgcgcaa
         2701 aaaagaaagc acatcgtagt catgctcatg cagataaagg caggtaagct ccggaaccac
         2761 cacagaaaaa gacaccattt ttctctcaaa catgtctgcg ggtttctgca taaacacaaa
         2821 ataaaataac aaaaaaacat ttaaacatta gaagcctgtc ttacaacagg aaaaacaacc
         2881 cttataagca taagacggac tacggccatg ccggcgtgac cgtaaaaaaa ctggtcaccg
         2941 tgattaaaaa gcaccaccga cagctcctcg gtcatgtccg gagtcataat gtaagactcg
         3001 gtaaacacat caggttgatt catcggtcag tgctaaaaag cgaccgaaat agcccggggg
         3061 aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt
         3121 aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc
         3181 ctcccgctcc agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca
         3241 gtaaaaaaga aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac
         3301 agtgtaaaaa agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg
         3361 ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc
         3421 caaaaaaccc acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc
         3481 attttaagaa aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca
         3541 cccgccccgt tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg
         3601 gcttcaatcc aaaataaggt atattattga tgatnnntta attaaggatc cnnncggtgt
         3661 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
         3721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
         3781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
         3841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
         3901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
         3961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
         4021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
         4081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
         4141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
         4201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
         4261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
         4321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
         4381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
         4441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
         4501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
         4561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
         4621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
         4681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
         4741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
         4801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
         4861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
         4921 agttcgccag ttaatagttt gcgcaacgtt gttgnnnnnn aaaaaggatc ttcacctaga
         4981 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
         5041 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
         5101 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
         5161 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
         5221 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
         5281 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
         5341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
         5401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
         5461 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
         5521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
         5581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
         5641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
         5701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
         5761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
         5821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
         5881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
         5941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
         6001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
         6061 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
         6121 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg
         6181 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
         6241 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
         6301 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga
         6361 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
         6421 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
         6481 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
         6541 taattaacat catcaataat atacctta
    //

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