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  • pDC316

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Microbix Biosystems
    載體名稱: pDC316
    質粒類型: 腺病毒表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: MCMV
    克隆方法: --
    載體大小: 3913 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素(Ampicillin)
    篩選標記: --
    備注: 克隆宿主菌DB3.1
    產品目錄號: --
    穩定性: --
    組成型: 組成型
    病毒/非病毒: 腺病毒

    載體質粒圖譜和多克隆位點信息

    pDC316載體圖譜



    載體簡介

    載體序列

    LOCUS       pDC316       3913 bp dsDNA    Circular
    DEFINITION  Puc based lox HCMV shuttle plasmid  for Cre-based Ad vector rescue Cummings & Graham, unpublished
    COMMENT     This file is best viewed with a free Vector NTI Viewer:
    COMMENT     http://www.informaxinc.com/view/viewer.html
    COMMENT     VNTREPLTYPE|Plasmid|
    COMMENT     VNTEXTCHREPL|Bacteria|
    FEATURES               Location/Qualifiers
         promoter        469..991
                         /note="MCMV promoter - Short"
                         /label=MCMV
         polyA_signal    1029..1188
                         /label=SV40\PolyA
         misc_feature    1190..1223
                         /label=loxP\
         rep_origin      1603..1903
                         /label=ori
         CDS             complement(2353..3169)
                         /label=amp
         misc_feature    3454..453
                         /label=Ad
         CDS             3823..1
                         /label=ITR
         CDS             complement(2..102)
                         /label=ITR
    ORIGIN
            1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
           61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
          121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
          181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
          241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
          301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
          361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
          421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 
          481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 
          541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 
          601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 
          661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 
          721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 
          781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 
          841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 
          901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 
          961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattccc cgggagatct aagcttgagc 
         1021 tcgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
         1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
         1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatccaa taacttcgta 
         1201 tagcatacat tatacgaagt tataagtagc ttggcgtaat catggtcata gctgtttcct 
         1261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
         1321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
         1381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
         1441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
         1501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
         1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
         1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
         1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
         1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
         1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 
         1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
         1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
         1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
         2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
         2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
         2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
         2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
         2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
         2341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
         2401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
         2461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
         2521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
         2581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
         2641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
         2701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
         2761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
         2821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
         2881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
         2941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
         3001 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 
         3061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
         3121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
         3181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
         3241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
         3301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
         3361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 
         3421 atgacattaa cctataaaaa taggcgtatc actctaggca aaatagcacc ctcccgctcc 
         3481 agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca gtaaaaaaga 
         3541 aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa 
         3601 agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca 
         3661 caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc 
         3721 acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc attttaagaa 
         3781 aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt 
         3841 tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc 
         3901 aaaataaggt ata 
    //
    

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