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  • pAAV-TRE-Syn-Fluc

    價格:3500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Addgene
    載體名稱: pAAV-TRE-Syn-Fluc
    質粒類型: 腺病毒載體;四環素誘導載體;熒光素酶報告載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: human Synapsin 1
    載體大小: 8361 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: 螢火蟲熒光素酶、橙色熒光蛋白
    克隆菌株: Stbl3 或 NEB Stble
    宿主細胞(系): 神經細胞
    備注: pAAV-TRE-Syn-Fluc載體含有human synapsin 1 啟動子,適合在神經細胞中表達。
    產品目錄號: #22907
    穩定性: 穩表達
    組成型/誘導型: 誘導型
    病毒/非病毒: 腺病毒

    載體質粒圖譜和多克隆位點信息

    pAAV-TRE-Syn-Fluc載體圖譜



    載體簡介

    載體序列

    LOCUS       KC152481                8361 bp    DNA     circular SYN 03-JUN-2013
    DEFINITION  Expression vector pAAV-TRE-Syn-Fluc, complete sequence.
    ACCESSION   KC152481
    VERSION     KC152481.1  GI:507593968
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     ORIGDB|GenBank
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTNAME|pAAV-TRE-Syn-Fluc|
      AUTHORS   Chen,Y., Cao,L., Luo,C., Ditzel,D.A., Peter,J. and Sprengel,R.
      TITLE     RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
      JOURNAL   Mol Ther Nucleic Acids 2, E85 (2013)
       PUBMED   23571608
      REMARK    Publication Status: Online-Only
    REFERENCE   2  (bases 1 to 8361)
      AUTHORS   Chen,Y. and Sprengel,R.
      TITLE     Direct Submission
      JOURNAL   Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
                institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
                Germany
    FEATURES             Location/Qualifiers
         source          1..8361
                         /organism="Expression vector pAAV-TRE-Syn-Fluc"
                         /mol_type="other DNA"
                         /db_xref="taxon:1337870"
         repeat_region   19..162
                         /note="ITR"
                         /rpt_type=inverted
                         /rpt_type=terminal
         misc_feature    245..542
                         /note="tetracycline responsive element: TRE"
         regulatory      543..559
                         /regulatory_class="promoter"
                         /note="CMVmin"
         misc_feature    616..748
                         /note="polyadenylation signal/transcriptional pause"
         regulatory      739..1190
                         /regulatory_class="promoter"
                         /note="Homo sapiens synapsin-1 promoter: Syn"
         misc_feature    1195..2847
                         /note="firefly luciferase"
         misc_feature    2848..2901
                         /note="2A peptide from Thosea asigna virus"
         misc_feature    2914..3762
                         /note="tTSKid"
         misc_feature    2914..3546
                         /note="Tet repressor: tetR[B/E]"
         misc_feature    3547..3564
                         /note="nuclear localization signal: NLS"
         misc_feature    3571..3756
                         /note="KRAB (Kruppel-associated box) domain; from Rat zinc
                         finger protein (kid-1)"
         misc_feature    3763..3816
                         /note="2A peptide from Thosea asigna virus"
         gene            3829..4485
                         /gene="mKO"
         CDS             3829..4485
                         /gene="mKO"
                         /note="fluorescent protein"
                         /codon_start=1
                         /transl_table=11
                         /product="monomeric Kusabira-orange"
                         /protein_id="AGM53873.1"
                         /db_xref="GI:507593969"
                         /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLR
                         VTMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGG
                         SASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDV
                         TMYLKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS
                         "
         ncRNA           4516..5112
                         /ncRNA_class="other"
                         /product="post-transcriptional regulatory element: WPRE"
         misc_feature    5145..5355
                         /note="bovine growth hormone polyA signal: bGH pA"
         repeat_region   complement(5365..5508)
                         /note="ITR"
                         /rpt_type=inverted
                         /rpt_type=terminal
         gene            6697..7359
                         /gene="Bla"
         CDS             6697..7359
                         /gene="Bla"
                         /note="ampicillin resistance"
                         /codon_start=1
                         /transl_table=11
                         /product="beta-lactamase"
                         /protein_id="AGM53874.1"
                         /db_xref="GI:507593970"
                         /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                         LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
                         EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
                         WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
                         HW"
    ORIGIN      
            1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
           61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
          121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
          181 ttctagtcct agggatatct tcgaaccatg gttaattaag tttaaacatc gatcaattcg
          241 agctcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
          301 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
          361 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
          421 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
          481 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
          541 acctatataa gcagagctcg gtacccgggg atcctctaga aatattaagg tacgggaggt
          601 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
          661 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
          721 gcaaaatagg ctgtccccag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
          781 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
          841 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
          901 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
          961 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
         1021 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
         1081 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
         1141 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga attcatggaa
         1201 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct
         1261 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
         1321 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
         1381 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
         1441 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
         1501 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
         1561 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
         1621 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
         1681 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
         1741 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact
         1801 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
         1861 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
         1921 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
         1981 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
         2041 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
         2101 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
         2161 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
         2221 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
         2281 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
         2341 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
         2401 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
         2461 atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
         2521 aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc
         2581 aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc
         2641 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
         2701 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
         2761 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
         2821 aagaagggcg gaaagtccaa attgctcgag ggcagaggaa gtcttctaac atgcggtgac
         2881 gtggaggaga atcccggccc tgcaccggga tccatgtcca gattagataa aagtaaagtg
         2941 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
         3001 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcacgtgcg caacaagcag
         3061 actcttatga acatgctttc agaggcaata ctggcgaagc atcacacccg ttcagcaccg
         3121 ttaccgactg agagttggca gcagtttctc caggaaaatg ctctgagttt ccgtaaagca
         3181 ttactggtcc atcgtgatgg agcccgattg catataggga cctctcctac gcccccccag
         3241 tttgaacaag cagaggcgca actacgctgt ctatgcgatg cagggttttc ggtcgaggag
         3301 gctcttttca ttctgcaatc tatcagccat tttacgttgg gtgcagtatt agaggagcaa
         3361 gcaacaaacc agatagaaaa taatcatgtg atagacgctg caccaccatt attacaagag
         3421 gcatttaata ttcaggcgag aacctctgct gaaatggcct tccatttcgg gctgaaatca
         3481 ttaatatttg gattttctgc acagttagat gaaaaaaagc atacacccat tgaggatggt
         3541 aataaaccaa aaaagaagag aaagctagca gtgtcagtga catttgaaga tgtggctgtg
         3601 ctctttactc gggacgagtg gaagaagctg gatctgtctc agagaagcct gtaccgtgag
         3661 gtgatgctgg agaattacag caacctggcc tccatggcag gattcctgtt taccaaacca
         3721 aaggtgatct ccctgttgca gcaaggagag gacccgtatc tcgagggcag aggaagtctt
         3781 ctaacatgcg gtgacgtgga ggagaatccc ggccctgcac cgggatccat ggtgagtgtg
         3841 attaaaccag aaatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc
         3901 acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc
         3961 gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc
         4021 tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta tttcaaacaa
         4081 gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct
         4141 tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc caaatttact
         4201 ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca
         4261 tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta
         4321 aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa
         4381 aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag gaaaaccgaa
         4441 ggcaacatta ctgagctggt agaagatgca gtagctcatt cctgatgtac aagtaaagcg
         4501 gccgcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
         4561 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
         4621 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
         4681 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
         4741 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
         4801 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
         4861 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
         4921 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
         4981 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
         5041 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
         5101 ccccgcctga tcgataccgt cgactagagc tcgctgatca gcctcgactg tgccttctag
         5161 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
         5221 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
         5281 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
         5341 caggcatgct ggggagagat ctgaggaacc cctagtgatg gagttggcca ctccctctct
         5401 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
         5461 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaacc cccccccccc
         5521 cccccctgca gcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
         5581 gcgtagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg
         5641 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
         5701 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
         5761 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
         5821 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
         5881 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
         5941 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
         6001 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
         6061 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
         6121 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
         6181 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
         6241 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
         6301 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
         6361 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
         6421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
         6481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
         6541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
         6601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
         6661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
         6721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
         6781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
         6841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
         6901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
         6961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
         7021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
         7081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
         7141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
         7201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
         7261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
         7321 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
         7381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
         7441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
         7501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
         7561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
         7621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
         7681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
         7741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
         7801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
         7861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
         7921 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
         7981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
         8041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
         8101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
         8161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
         8221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
         8281 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
         8341 attcattaat gcaggctgca g
    //
    

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