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  • pAAV-TRE-CAG-Fluc

    價格:3500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: --
    載體名稱: pAAV-TRE-CAG-Fluc
    質粒類型: 腺病毒載體;四環素誘導載體;熒光素酶報告載體;熒光蛋白報告載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: CAG
    載體大小: 8687 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: 螢火蟲熒光素酶、橙色熒光蛋白
    克隆菌株: Stbl3 或 NEB Stble
    宿主細胞(系): 神經細胞
    備注: --
    產品目錄號: --
    穩定性: 穩表達
    組成型/誘導型: 誘導型
    病毒/非病毒: 腺病毒

    載體質粒圖譜和多克隆位點信息

    pAAV-TRE-CAG-Fluc載體圖譜



    載體簡介

    載體序列

    LOCUS       KC152482                8687 bp    DNA     circular SYN 03-JUN-2013
    DEFINITION  Expression vector pAAV-TRE-CAG-Fluc, complete sequence.
    ACCESSION   KC152482
    VERSION     KC152482.1  GI:507593971
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     ORIGDB|GenBank
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTNAME|pAAV-TRE-CAG-Fluc|
       PUBMED   23571608
      REMARK    Publication Status: Online-Only
    REFERENCE   2  (bases 1 to 8687)
      AUTHORS   Chen,Y. and Sprengel,R.
      JOURNAL   Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
                institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
                Germany
    FEATURES             Location/Qualifiers
         source          1..8687
                         /organism="Expression vector pAAV-TRE-CAG-Fluc"
                         /mol_type="other DNA"
                         /db_xref="taxon:1337869"
         repeat_region   19..162
                         /note="AAV2 ITR"
                         /rpt_type=inverted
                         /rpt_type=terminal
         misc_feature    245..542
                         /note="tetracycline responsive element: TRE"
         regulatory      543..559
                         /regulatory_class="promoter"
                         /note="CMVmin"
         regulatory      564..1504
                         /regulatory_class="promoter"
                         /note="CMV earlyenhancer/chicken beta actin promoter: CAG"
         misc_feature    1521..3173
                         /note="firefly luciferase"
         misc_feature    3174..3227
                         /note="2A peptide from Thosea asigna virus"
         misc_feature    3240..4088
                         /note="tTSKid"
         misc_feature    3240..3872
                         /note="Tet repressor: tetR[B/E]"
         misc_feature    3873..3890
                         /note="nuclear localization signal: NLS"
         misc_feature    3897..4082
                         /note="KRAB (Kruppel-associated box) domain; from Rat zinc
                         finger protein (kid-1)"
         misc_feature    4089..4142
                         /note="2A peptide from Thosea asigna virus"
         gene            4155..4811
                         /gene="mKO"
         CDS             4155..4811
                         /gene="mKO"
                         /note="fluorescent protein"
                         /codon_start=1
                         /transl_table=11
                         /product="monomeric Kusabira-orange"
                         /protein_id="AGM53875.1"
                         /db_xref="GI:507593972"
                         /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLR
                         VTMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGG
                         SASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDV
                         TMYLKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS
                         "
         ncRNA           4842..5438
                         /ncRNA_class="other"
                         /product="post-transcriptional regulatory element: WPRE"
         misc_feature    5471..5681
                         /note="bovine growth hormone polyA signal: bGH pA"
         repeat_region   complement(5691..5834)
                         /note="AAV2 ITR"
                         /rpt_type=inverted
                         /rpt_type=terminal
         gene            7023..7685
                         /gene="Bla"
         CDS             7023..7685
                         /gene="Bla"
                         /note="ampicillin resistance"
                         /codon_start=1
                         /transl_table=11
                         /product="beta-lactamase"
                         /protein_id="AGM53876.1"
                         /db_xref="GI:507593973"
                         /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                         LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
                         EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
                         WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
                         HW"
    ORIGIN      
            1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
           61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
          121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
          181 ttctagtcct agggatatct tcgaaccatg gttaattaag tttaaacatc gatcaattcg
          241 agctcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
          301 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
          361 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
          421 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
          481 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
          541 acctatataa gcagagctcg gtaccattga cgtcaataat gacgtatgtt cccatagtaa
          601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
          661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
          721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
          781 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
          841 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
          901 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg
          961 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
         1021 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
         1081 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
         1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
         1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
         1261 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
         1321 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
         1381 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
         1441 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
         1501 gatctagtcg attcgaattc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
         1561 tctatccgct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg
         1621 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg
         1681 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
         1741 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
         1801 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
         1861 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc
         1921 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt
         1981 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
         2041 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac
         2101 tgataatgaa ctcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa
         2161 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
         2221 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
         2281 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt
         2341 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat
         2401 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
         2461 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc
         2521 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg
         2581 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
         2641 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag
         2701 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
         2761 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
         2821 tagttgaccg cttgaagtct ttaattaaat acaaaggata ccaggtggcc cccgctgaat
         2881 tggagtcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg
         2941 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
         3001 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
         3061 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
         3121 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg ctcgagggca
         3181 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctgca ccgggatcca
         3241 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
         3301 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
         3361 tgtattggca cgtgcgcaac aagcagactc ttatgaacat gctttcagag gcaatactgg
         3421 cgaagcatca cacccgttca gcaccgttac cgactgagag ttggcagcag tttctccagg
         3481 aaaatgctct gagtttccgt aaagcattac tggtccatcg tgatggagcc cgattgcata
         3541 tagggacctc tcctacgccc ccccagtttg aacaagcaga ggcgcaacta cgctgtctat
         3601 gcgatgcagg gttttcggtc gaggaggctc ttttcattct gcaatctatc agccatttta
         3661 cgttgggtgc agtattagag gagcaagcaa caaaccagat agaaaataat catgtgatag
         3721 acgctgcacc accattatta caagaggcat ttaatattca ggcgagaacc tctgctgaaa
         3781 tggccttcca tttcgggctg aaatcattaa tatttggatt ttctgcacag ttagatgaaa
         3841 aaaagcatac acccattgag gatggtaata aaccaaaaaa gaagagaaag ctagcagtgt
         3901 cagtgacatt tgaagatgtg gctgtgctct ttactcggga cgagtggaag aagctggatc
         3961 tgtctcagag aagcctgtac cgtgaggtga tgctggagaa ttacagcaac ctggcctcca
         4021 tggcaggatt cctgtttacc aaaccaaagg tgatctccct gttgcagcaa ggagaggacc
         4081 cgtatctcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
         4141 ctgcaccggg atccatggtg agtgtgatta aaccagaaat gaagatgagg tactacatgg
         4201 acggctccgt caatgggcat gagttcacaa ttgaaggtga aggcacaggc agaccttacg
         4261 agggacatca agagatgaca ctacgcgtca caatggccaa gggcgggcca atgcctttcg
         4321 cgtttgactt agtgtcacac gtgttctgtt acggccacag accttttact aaatatccag
         4381 aagagatacc agactatttc aaacaagcat ttcctgaagg cctgtcatgg gaaaggtcgt
         4441 tggagttcga agatggtggg tccgcttcag tcagtgcgca tataagcctt agaggaaaca
         4501 ccttctacca caaatccaaa tttactgggg ttaactttcc tgccgatggt cctatcatgc
         4561 aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat tactgccagc gacggagttc
         4621 tgaagggtga tgttacgatg tacctaaaac ttgaaggagg cggcaatcac aaatgccaat
         4681 tcaagactac ttacaaggcg gcaaaaaaga ttcttaaaat gccaggaagc cattacatca
         4741 gccatcgcct cgtcaggaaa accgaaggca acattactga gctggtagaa gatgcagtag
         4801 ctcattcctg atgtacaagt aaagcggccg caagcttatc gataatcaac ctctggatta
         4861 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
         4921 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
         4981 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
         5041 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
         5101 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
         5161 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
         5221 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
         5281 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
         5341 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
         5401 gagtcggatc tccctttggg ccgcctcccc gcctgatcga taccgtcgac tagagctcgc
         5461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
         5521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
         5581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
         5641 aagggggagg attgggaaga caatagcagg catgctgggg agagatctga ggaaccccta
         5701 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgcccgggca
         5761 aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
         5821 gagggagtgg ccaacccccc cccccccccc cctgcagcct ggcgtaatag cgaagaggcc
         5881 cgcaccgatc gcccttccca acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc
         5941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
         6001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
         6061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
         6121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
         6181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
         6241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
         6301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
         6361 tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt
         6421 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
         6481 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
         6541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
         6601 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
         6661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
         6721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
         6781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
         6841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
         6901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
         6961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
         7021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
         7081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
         7141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
         7201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
         7261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
         7321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
         7381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
         7441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
         7501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
         7561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
         7621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
         7681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
         7741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
         7801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
         7861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
         7921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
         7981 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
         8041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
         8101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
         8161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
         8221 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
         8281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
         8341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
         8401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
         8461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
         8521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
         8581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
         8641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gctgcag
    //
    

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