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  • pAAV-RC

    價格:800元

    聯系方式:I47-825O-882O

    買家導航

    pAAVRC載體基本信息

    出品公司: Agilent
    載體名稱: pAAVRC, pAAV-RC, pAAV RC
    質粒類型: 腺病毒相關載體
    表達水平:
    啟動子: CMV
    載體大小: 7327 bp
    GenBank序列號: AF369963
    載體標簽: --
    載體抗性: 氨芐
    備注: --
    產品目錄號: 240071
    穩定性: /
    組成型: --
    病毒/非病毒: 腺病毒

    pAAVRC載體質粒圖譜和多克隆位點信息

    pAAV-RC載體圖譜

    pAAV-RC載體特征

    pAAVRC載體簡介

    pAAVRC載體序列

    LOCUS       pAAV-RC                7327 bp    DNA     circular SYN 22-JUL-2001
    DEFINITION  Cloning vector pAAV-RC, complete sequence.
    ACCESSION   AF369963
    VERSION     AF369963.1  GI:14994089
    KEYWORDS    .
    SOURCE      Cloning vector pAAV-RC
      ORGANISM  Cloning vector pAAV-RC
                other sequences; artificial sequences; vectors.
    REFERENCE   1  (bases 1 to 7327)
      AUTHORS   Grafsky,A.J. III.
      TITLE     pAAV-RC for AAV Helper-Free System
      JOURNAL   Unpublished
    REFERENCE   2  (bases 1 to 7327)
      AUTHORS   Grafsky,A.J. III.
      TITLE     Direct Submission
      JOURNAL   Submitted (16-APR-2001) Technical Services, Stratagene, 11011 N.
                Torrey Pines Rd., La Jolla, CA 92037, USA
    FEATURES             Location/Qualifiers
         source          1..7327
                         /organism="Cloning vector pAAV-RC"
                         /mol_type="genomic DNA"
                         /db_xref="taxon:161364"
                         /lab_host="Escherichia coli"
                         /note="used for recombinant DNA processing"
         CDS             131..1996
                         /note="AAV-2 rep"
                         /codon_start=1
                         /transl_table=11
                         /product="adeno-associated virus 2 rep protein"
                         /protein_id="AAK76418.1"
                         /db_xref="GI:14994090"
         CDS             2013..4220
                         /note="cap VP1"
                         /codon_start=1
                         /transl_table=11
                         /product="capsid protein VP1"
                         /protein_id="AAK76419.1"
                         /db_xref="GI:14994091"
         rep_origin      4705..5161
                         /note="f1 origin"
         CDS             5292..6152
                         /note="confers ampicillin resistance"
                         /codon_start=1
                         /transl_table=11
                         /product="beta-lactamase"
                         /protein_id="AAK76420.1"
                         /db_xref="GI:14994092"
         rep_origin      6161..7019
                         /note="ColE1 origin"
    ORIGIN      
            1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa
           61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg
          121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga
          181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt
          241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
          301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct
          361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac
          421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat
          481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac
          541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
          601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag
          661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc
          721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag
          781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac
          841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
          901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac
          961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg
         1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct
         1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac
         1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
         1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg
         1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag
         1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat
         1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca
         1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
         1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt
         1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc
         1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac
         1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca
         1741 cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc
         1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc
         1861 tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat
         1921 gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg
         1981 catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt
         2041 ggctcgagga cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac
         2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt
         2161 acaagtacct cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg
         2221 ccgcggccct cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt
         2281 acctcaagta caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt
         2341 ttgggggcaa cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg
         2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc
         2461 ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa
         2521 gattgaattt tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac
         2581 agccaccagc agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac
         2641 caatggcaga caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt
         2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc
         2761 ccacctacaa caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca
         2821 atcactactt tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc
         2881 acttttcacc acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga
         2941 gactcaactt caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga
         3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc
         3061 tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct
         3121 tcatggtgcc acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct
         3181 cttcatttta ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta
         3241 ccttcagcta cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc
         3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc agaacaaaca
         3361 ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc tcaggccgga gcgagtgaca
         3421 ttcgggacca gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa
         3481 agacatctgc ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc
         3541 tcaatggcag agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg
         3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa
         3661 caaatgtgga cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc
         3721 ccgtggctac ggagcagtat ggttctgtat ctaccaacct ccagagaggc aacagacaag
         3781 cagctaccgc agatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag
         3841 atgtgtacct tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc
         3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga
         3961 acaccccggt acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca
         4021 tcacacagta ctccacggga caggtcagcg tggagatcga gtgggagctg cagaaggaaa
         4081 acagcaaacg ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg
         4141 tggactttac tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat
         4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg
         4261 aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc
         4321 atggcgggtt aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt
         4381 cgtgacgtga attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt
         4441 ttgcgacatt ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca
         4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact ggccgtcgtt
         4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
         4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
         4681 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
         4741 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
         4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
         4861 ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
         4921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
         4981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
         5041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
         5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
         5161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
         5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
         5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
         5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
         5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
         5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
         5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
         5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
         5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
         5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
         5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
         5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
         5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
         5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
         6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
         6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
         6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
         6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
         6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
         6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
         6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
         6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
         6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
         6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
         6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
         6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
         6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
         6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
         6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
         6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
         6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
         7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
         7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
         7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
         7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
         7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
         7321 acgccaa
    //
    

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