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  • pAAV-CMV-Rluc

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: --
    載體名稱: pAAV-CMV-Rluc
    質粒類型: 腺病毒載體;綠色海腎熒光素酶報告載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: CMV
    載體大小: 6824 bp
    5' 測序引物及序列: pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: 綠色海腎熒光素酶
    克隆菌株: Stbl3 或 NEB Stble
    宿主細胞(系): 常規哺乳動物細胞系
    備注: --
    產品目錄號: --
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 腺病毒

    載體質粒圖譜和多克隆位點信息

    pAAV-CMV-Rluc載體圖譜



    載體簡介

    載體序列

    LOCUS       KC152484                6824 bp    DNA     circular SYN 03-JUN-2013
    DEFINITION  Expression vector pAAV-CMV-Rluc, complete sequence.
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     ORIGDB|GenBank
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTNAME|pAAV-CMV-Rluc|
      AUTHORS   Chen,Y., Cao,L., Luo,C., Ditzel,D.A., Peter,J. and Sprengel,R.
      TITLE     RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
      JOURNAL   Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
                institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
                Germany
    FEATURES             Location/Qualifiers
         source          1..6824
                         /organism="Expression vector pAAV-CMV-Rluc"
                         /mol_type="other DNA"
                         /db_xref="taxon:1337867"
         repeat_region   19..162
                         /note="AAV2 ITR"
                         /rpt_type=inverted
                         /rpt_type=terminal
         regulatory      429..955
                         /regulatory_class="promoter"
                         /note="CMV promoter"
         intron          1069..1205
                         /note="chimeric intron"
         misc_feature    1235..2167
                         /note="Renilla luciferase"
         misc_feature    2171..2224
                         /note="2A peptide from Thosea asigna virus"
         CDS             2240..2959
                         /note="fluorescent protein"
                         /codon_start=1
                         /transl_table=11
                         /product="Venus"
                         /protein_id="AGM53879.1"
                         /db_xref="GI:507593978"
                         /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                         LKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                         DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN
                         GIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                         MVLLEFVTAAGITLGMDELYK"
         ncRNA           2979..3575
                         /ncRNA_class="other"
                         /note="post-transcriptional regulatory element: WPRE"
         misc_feature    3608..3818
                         /note="bovine growth hormone polyA signal: bGH pA"
         repeat_region   complement(3828..3971)
                         /note="AAV2 ITR"
                         /rpt_type=inverted
                         /rpt_type=terminal
         gene            5160..5822
                         /gene="Bla"
         CDS             5160..5822
                         /gene="Bla"
                         /note="ampicillin resistance"
                         /codon_start=1
                         /transl_table=11
                         /product="beta-lactamase"
                         /protein_id="AGM53880.1"
                         /db_xref="GI:507593979"
                         /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                         LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
                         EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
                         WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
                         HW"
    ORIGIN      
            1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
           61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
          121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
          181 ttctagacct tttgctcaca tggctcgaca gatcttcaat attggccatt agccatatta
          241 ttcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
          301 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttggcattga
          361 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
          421 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
          481 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
          541 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
          601 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
          661 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
          721 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
          781 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
          841 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
          901 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcact
          961 agaagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag tgcttctgac
         1021 acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc
         1081 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
         1141 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
         1201 acaggtgtcc actcccagtt cagaattcgg atccatgact tcgaaagttt atgatccaga
         1261 acaaaggaaa cggatgataa ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt
         1321 tcttgattca tttattaatt attatgattc agaaaaacat gcagaaaatg ctgttatttt
         1381 tttacatggt aacgcggcct cttcttattt atggcgacat gttgtgccac atattgagcc
         1441 agtagcgcgg tgtattatac cagaccttat tggtatgggc aaatcaggca aatctggtaa
         1501 tggttcttat aggttacttg atcattacaa atatcttact gcatggtttg aacttcttaa
         1561 tttaccaaag aagatcattt ttgtcggcca tgattggggt gcttgtttgg catttcatta
         1621 tagctatgag catcaagata agatcaaagc aatagttcac gctgaaagtg tagtagatgt
         1681 gattgaatca tgggatgaat ggcctgatat tgaagaagat attgcgttga tcaaatctga
         1741 agaaggagaa aaaatggttt tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa
         1801 aatcatgaga aagttagaac cagaagaatt tgcagcatat cttgaaccat tcaaagagaa
         1861 aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa atcccgttag taaaaggtgg
         1921 taaacctgac gttgtacaaa ttgttaggaa ttataatgct tatctacgtg caagtgatga
         1981 tttaccaaaa atgtttattg aatcggaccc aggattcttt tccaatgcta ttgttgaagg
         2041 tgccaagaag tttcctaata ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga
         2101 agatgcacct gatgaaatgg gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa
         2161 tgaacaactc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
         2221 ccctgcaccg ggatccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
         2281 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
         2341 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
         2401 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
         2461 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
         2521 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
         2581 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
         2641 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
         2701 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
         2761 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
         2821 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
         2881 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
         2941 ggacgagctg tacaagtaaa gcggccgcaa gcttatcgat aatcaacctc tggattacaa
         3001 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
         3061 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
         3121 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
         3181 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
         3241 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
         3301 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
         3361 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
         3421 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
         3481 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
         3541 tcggatctcc ctttgggccg cctccccgcc tgatcgatac cgtcgactag agctcgctga
         3601 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
         3661 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
         3721 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
         3781 ggggaggatt gggaagacaa tagcaggcat gctggggaga gatctgagga acccctagtg
         3841 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
         3901 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
         3961 ggagtggcca accccccccc ccccccccct gcagcctggc gtaatagcga agaggcccgc
         4021 accgatcgcc cttcccaaca gttgcgtagc ctgaatggcg aatggcgcga cgcgccctgt
         4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
         4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
         4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
         4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
         4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
         4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
         4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
         4501 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg
         4561 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
         4621 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
         4681 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
         4741 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
         4801 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
         4861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
         4921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
         4981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
         5041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
         5101 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
         5161 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
         5221 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
         5281 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
         5341 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
         5401 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
         5461 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
         5521 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
         5581 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
         5641 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
         5701 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
         5761 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
         5821 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
         5881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
         5941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
         6001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
         6061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
         6121 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
         6181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
         6241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
         6301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
         6361 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
         6421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
         6481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
         6541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
         6601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
         6661 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
         6721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
         6781 cgcctctccc cgcgcgttgg ccgattcatt aatgcaggct gcag
    //
    

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