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  • pSP64-Tol2-Transposase

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: --
    載體名稱: pSP64-Tol2-Transposase
    質粒類型: 轉座子載體
    高拷貝/低拷貝: --
    克隆方法: --
    啟動子: SP6
    載體大小: 5142bp
    5' 測序引物及序列: sp6:ATTTAGGTGACACTATAGAA
    3' 測序引物及序列: M13R
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: DH5α 等
    宿主細胞(系): 哺乳細胞
    備注: --
    產品目錄號: --
    穩定性: 瞬表達
    組成型/誘導型: --
    病毒/非病毒: --

    載體質粒圖譜和多克隆位點信息

    pSP64-Tol2-Transposase載體圖譜

    載體簡介

    載體序列

    LOCUS       pSP64-Tol2-Transposase               5142 bp ds-DNA     circular SYN 13-FEB-2019
    DEFINITION  synthetic circular DNA
    KEYWORDS    pSP64-Tol2-Transposase
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 5142)
      AUTHORS   Triple Threat
      TITLE     Direct Submission
    FEATURES             Location/Qualifiers
         source          1..5142
                         /organism="synthetic DNA construct"
                         /mol_type="other DNA"
         CDS             28..2115
                         /codon_start=1
                         /label=Transposase Tol2
                         /translation="MFIGPLEVTSCHIYYHNAQHLDLEIREIITVNQWKKMEEVCDSSA
                         AASSTVQNQPQDQEHPWPYLREFFSLSGVNKDSFKMKCVLCLPLNKEISAFKSSPSNLR
                         KHIERMHPNYLKNYSKLTAQKRKIGTSTHASSSKQLKVDSVFPVKHVSPVTVNKAILRY
                         IIQGLHPFSTVDLPSFKELISTLQPGISVITRPTLRSKIAEAALIMKQKVTAAMSEVEW
                         IATTTDCWTARRKSFIGVTAHWINPGSLERHSAALACKRLMGSHTFEVLASAMNDIHSE
                         YEIRDKVVCTTTDSGSNFMKAFRVFGVENNDIETEARRCESDDTDSEGCGEGSDGVEFQ
                         DASRVLDQDDGFEFQLPKHQKCACHLLNLVSSVDAQKALSNEHYKKLYRSVFGKCQALW
                         NKSSRSALAAEAVESESRLQLLRPNQTRWNSTFMAVDRILQICKEAGEGALRNICTSLE
                         VPMFNPAEMLFLTEWANTMRPVAKVLDILQAETNTQLGWLLPSVHQLSLKLQRLHHSLR
                         YCDPLVDALQQGIQTRFKHMFEDPEIIAAAILLPKFRTSWTNDETIIKRGMDYIRVHLE
                         PLDHKKELANSSSDDEDFFASLKPTTHEASKELDGYLACVSDTRESLLTFPAICSLSIK
                         TNTPLPASAACERLFSTAGLLFSPKRARLDTNNFENQLLLKLNLRFYNFEGGGKKKRKV
                         G"
         primer_bind     complement(2212..2228)
                         /label=M13 rev
                         /note="common sequencing primer, one of multiple similar 
                         variants"
         rep_origin      complement(2614..3202)
                         /direction=LEFT
                         /label=ori
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(3373..4233)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /label=AmpR
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(4234..4338)
                         /gene="bla"
                         /label=AmpR promoter
         misc_feature    join(5126..5142,1..2)
                         /label=SP6 promoter
    BASE COUNT         1401 a         1255 c         1227 g         1258 t
    ORIGIN
            1 gaatacaagc ttgggctgca ggtcgacatg ttcattggtc ctttggaagt gacgtcatgt
           61 cacatctatt accacaatgc acagcacctt gacctggaaa ttagggaaat tataacagtc
          121 aatcagtgga agaaaatgga ggaagtatgt gattcatcag cagctgcgag cagcacagtc
          181 caaaatcagc cacaggatca agagcacccg tggccgtatc ttcgcgaatt cttttcttta
          241 agtggtgtaa ataaagattc attcaagatg aaatgtgtcc tctgtctccc gcttaataaa
          301 gaaatatcgg ccttcaaaag ttcgccatca aacctaagga agcatattga gagaatgcac
          361 ccaaattacc tcaaaaacta ctctaaattg acagcacaga agagaaagat cgggacctcc
          421 acccatgctt ccagcagtaa gcaactgaaa gttgactcag ttttcccagt caaacatgtg
          481 tctccagtca ctgtgaacaa agctatatta aggtacatca ttcaaggact tcatcctttc
          541 agcactgttg atctgccatc atttaaagag ctgattagta cactgcagcc tggcatttct
          601 gtcattacaa ggcctacttt acgctccaag atagctgaag ctgctctgat catgaaacag
          661 aaagtgactg ctgccatgag tgaagttgaa tggattgcaa ccacaacgga ttgttggact
          721 gcacgtagaa agtcattcat tggtgtaact gctcactgga tcaaccctgg aagtcttgaa
          781 agacattccg ctgcacttgc ctgcaaaaga ttaatgggct ctcatacttt tgaggtactg
          841 gccagtgcca tgaatgatat ccactcagag tatgaaatac gtgacaaggt tgtttgcaca
          901 accacagaca gtggttccaa ctttatgaag gctttcagag tttttggtgt ggaaaacaat
          961 gatatcgaga ctgaggcaag aaggtgtgaa agtgatgaca ctgattctga aggctgtggt
         1021 gagggaagtg atggtgtgga attccaagat gcctcacgag tcctggacca agacgatggc
         1081 ttcgaattcc agctaccaaa acatcaaaag tgtgcctgtc acttacttaa cctagtctca
         1141 agcgttgatg cccaaaaagc tctctcaaat gaacactaca agaaactcta cagatctgtc
         1201 tttggcaaat gccaagcttt atggaataaa agcagccgat cggctctagc agctgaagct
         1261 gttgaatcag aaagccggct tcagctttta aggccaaacc aaacgcggtg gaattcaact
         1321 tttatggctg ttgacagaat tcttcaaatt tgcaaagaag caggagaagg cgcacttcgg
         1381 aatatatgca cctctcttga ggttccaatg tttaatccag cagaaatgct gttcttgaca
         1441 gagtgggcca acacaatgcg tccagttgca aaagtactcg acatcttgca agcggaaacg
         1501 aatacacagc tggggtggct gctgcctagt gtccatcagt taagcttgaa acttcagcga
         1561 ctccaccatt ctctcaggta ctgtgaccca cttgtggatg ccctacaaca aggaatccaa
         1621 acacgattca agcatatgtt tgaagatcct gagatcatag cagctgccat ccttctccct
         1681 aaatttcgga cctcttggac aaatgatgaa accatcataa aacgaggcat ggactacatc
         1741 agagtgcatc tggagccttt ggaccacaag aaggaattgg ccaacagttc atctgatgat
         1801 gaagattttt tcgcttcttt gaaaccgaca acacatgaag ccagcaaaga gttggatgga
         1861 tatctggcct gtgtttcaga caccagggag tctctgctca cgtttcctgc tatttgcagc
         1921 ctctctatca agactaatac acctcttccc gcatcggctg cctgtgagag gcttttcagc
         1981 actgcaggat tgcttttcag ccccaaaaga gctaggcttg acactaacaa ttttgagaat
         2041 cagcttctac tgaagttaaa tctgaggttt tacaactttg agggcggcgg caagaagaag
         2101 cgcaaggtgg gctagggatc cccgggcgag ctcccaaaaa aaaaaaaaaa aaaaaaaaaa
         2161 aaaaaccgaa ttctctagag gtaccccatg gctcgaggaa ttcgtaatca tgtcatagct
         2221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
         2281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
         2341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
         2401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
         2461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
         2521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
         2581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
         2641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
         2701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
         2761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
         2821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
         2881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
         2941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
         3001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
         3061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
         3121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
         3181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
         3241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
         3301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
         3361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
         3421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
         3481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
         3541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
         3601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
         3661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
         3721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
         3781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
         3841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
         3901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
         3961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
         4021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
         4081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
         4141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
         4201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
         4261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
         4321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
         4381 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
         4441 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
         4501 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
         4561 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc
         4621 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
         4681 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
         4741 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
         4801 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
         4861 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga
         4921 ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc
         4981 caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc
         5041 cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca
         5101 taaccttatg tatcatacac atacgattta ggtgacacta ta
    //
    
    
    

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