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  • pRU668

    價格:6800元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    載體名稱: pRU668
    來源歷史: <- R. Schmitt
    質?剐:
    incT, Km抗性, Tc抗性, tra+, ts
    載體說明: 將天然存在的轉座子Tn1721(11.4 kb)插入體內接合的Kmr ts質粒Rts1(Rts1::Tn1721);Tn1721本身屬于Tn3家族,由一個重復的tet區和一個“小轉座子”(Tn1722)組成,后者編碼轉座子功能;可用于大腸桿菌的誘變;大尺寸Rts1(217kb)的缺失阻止了任何Rts1::Tn復制子的共轉化。
    宿主菌: Escherichia coli K12 DH1 RU2945
    培養條件: LB培養基,30度
    參考文獻: Altenbuchner, J., Schmid, K., Schmitt, R. (1983). Tn1721-encoded tetracycline resistance: mapping of structural and functional genes mediating resistance. J.Bacteriol. 153 : 116-123 .
    Heffron, F., (1983). Tn3 and its relatives. In Mobile Genetic Elements , pp. 223-260. Edited by J. A. Shapiro, Academic Press.
    Schmitt, R., Altenbuchner, J., Wiebauer, K., Arnold, W., Pühler, A., Schöffl, F. (1981). Basis of transposition and gene amplification by Tn1721 and related tetracycline resistance transposons. Cold Spring Harb.Symp.Quant.Biol. 45 : 59-65 .
    Schmitt, R., Bernhard, E., Mattes, R. (1979). Characterization of Tn1721, a new transposon containing a tetracycline gene capable of amplification. Mol.Gen.Genet. 172 : 53-65 .
    Terawaki, Y., Kobayashi, Y., Matsumoto, H., Kamio, Y. (1981). Molecular cloning and mapping of a deletion derivative of the plasmid Rts1. Plasmid 6 : 222-234 .
    Ubben, D., Schmitt, R. (1986). Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. Gene 41 : 145-152 .
    安全等級: 1級
     分離國家: 原產國未知
    存儲時間: 1990年3月16日前

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