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  • pNL3.2[NlucP/minP]

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Promega
    載體名稱: pNL3.2[NlucP/minP]
    質粒類型: 熒光素酶載體
    高拷貝/低拷貝: --
    克隆方法: --
    啟動子: SV40
    載體大小: 3274 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: --
    宿主細胞(系): --
    備注: 帶有最小啟動子的載體,用于使用不穩定的NanoLuc®熒光素酶測量轉錄反應元件的活性。
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    載體質粒圖譜和多克隆位點信息

    pNL3.2[NlucPminP]載體圖譜

    載體簡介

    載體序列

    LOCUS       pNL3.2[NlucP minP]                3274 bp ds-DNA     circular SYN 26-JUL-2013
    DEFINITION  Vector with a minimal promoter for measuring the activity of 
                transcriptional response elements using destabilized NanoLuc(R) 
                luciferase.
    ACCESSION   JQ513370
    VERSION     .
    KEYWORDS    pNL3.2[NlucP minP]
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 3274)
      AUTHORS   Promega
      TITLE     Direct Submission
      JOURNAL   Exported Saturday, June 6, 2020 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..3274
                         /organism="synthetic DNA construct"
                         /lab_host="Mammalian Cells"
                         /mol_type="other DNA"
         misc_feature    1..70
                         /note="MCS"
                         /note="multiple cloning site"
         promoter        77..108
                         /note="minP"
                         /note="minimal TATA-box promoter with low basal activity"
         CDS             141..653
                         /codon_start=1
                         /product="NanoLuc(R) luciferase"
                         /note="Nluc"
                         /note="human codon-optimized"
                         /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                         IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                         TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                         RLCERILA"
         CDS             657..776
                         /codon_start=1
                         /product="PEST degradation sequence from mouse ornithine 
                         decarboxylase"
                         /note="hPEST"
                         /note="human codon-optimized"
                         /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
         polyA_signal    828..949
                         /note="SV40 poly(A) signal"
                         /note="SV40 polyadenylation signal"
         rep_origin      complement(1368..1956)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(2156..3016)
                         /codon_start=1
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         polyA_signal    3121..3169
                         /note="synthetic polyadenylation signal"
         misc_feature    3183..3274
                         /note="pause site"
                         /note="RNA polymerase II transcriptional pause signal from 
                         the human alpha-2 globin gene"
    ORIGIN
            1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
           61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
          121 gtactgttgg taaagccacc atggtcttca cactcgaaga tttcgttggg gactggcgac
          181 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
          241 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
          301 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
          361 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
          421 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
          481 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
          541 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
          601 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgaattctc
          661 acggctttcc gcctgaggtt gaagagcaag ccgccggtac attgcctatg tcctgcgcac
          721 aagaaagcgg tatggaccgg cacccagccg cttgtgcttc agctcgcatc aacgtctaag
          781 gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga
          841 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
          901 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
          961 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
         1021 aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct
         1081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
         1141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg
         1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
         1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
         1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
         1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
         1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
         1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
         1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
         1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
         1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
         1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
         1801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
         1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
         1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
         1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
         2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
         2101 atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc
         2161 agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg
         2221 cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg
         2281 cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc
         2341 cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta
         2401 tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg
         2461 tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact
         2521 ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca
         2581 gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg
         2641 taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga
         2701 ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt
         2761 gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca
         2821 tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt
         2881 cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt
         2941 cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa
         3001 aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat cagggttact
         3061 agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc
         3121 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
         3181 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
         3241 cccagtgcaa gtgcaggtgc cagaacattt ctct
    //
    
    
    

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