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  • pKOR1

    價格:6000元

    聯系方式:I47-825O-882O

    買家導航

    pKORI金黃色葡萄球菌基因敲除載體基本信息

    出品公司: --
    載體名稱: pKOR1
    質粒類型: 金黃色葡萄球菌基因敲除載體,大腸桿菌-金黃色葡萄球菌穿梭載體;金葡菌基因敲除載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: Gateway克隆
    啟動子: --
    載體大小: 10030 bp
    5' 測序引物及序列: 自己合成
    3' 測序引物及序列: 自己合成
    載體標簽: --
    載體抗性: 氨芐青霉素/氯霉素
    篩選標記: 氯霉素(10 ug/ml)
    克隆菌株: DH5α 等
    宿主細胞(系): 金黃色葡萄球菌
    備注: pKOR1是大腸桿菌-金黃色葡萄球菌穿梭載體,主要用于金葡菌的基因敲除。pKOR1金黃色葡萄球菌基因敲除載體,在大腸桿菌中的抗性為Amp,在金葡菌中篩選抗性為氯霉素(10ug/ml).

    生物風對金黃色葡萄球菌的基因敲除具有豐富的實驗經驗,并且成功敲除過很多金黃色葡萄球菌基因,請有需要對金黃色葡萄球菌進行基因操作技術服務的老師和我們聯系。
    產品目錄號: --
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    pKORI金黃色葡萄球菌基因敲除載體質粒圖譜和多克隆位點信息

    pKOR1載體圖譜
    pKOR1載體圖譜


    pKOR1金黃色葡萄球菌基因敲除實驗流程圖:

    pKOR1載體圖譜

    pKORI金黃色葡萄球菌基因敲除載體簡介

    pKOR1是大腸桿菌-金黃色葡萄球菌穿梭載體,主要用于金葡菌的基因敲除。

    pKOR1金黃色葡萄球菌基因敲除載體,在大腸桿菌中的抗性為Amp,在金葡菌中篩選抗性為氯霉素(10ug/ml).

    生物風對金黃色葡萄球菌的基因敲除具有豐富的實驗經驗,并且成功敲除過很多金黃色葡萄球菌基因,請有需要對金黃色葡萄球菌進行基因操作技術服務的老師和我們聯系。

    pKORI金黃色葡萄球菌基因敲除載體序列

    LOCUS       pKOR1                  10030 bp    DNA     circular      2-OCT-2015
    SOURCE      
      ORGANISM  
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTDATE|705424862|
    COMMENT     VNTDBDATE|746737717|
    COMMENT     LSOWNER|
    COMMENT     VNTNAME|pKOR1|
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         primer          9998..10015
                         /vntifkey="27"
                         /label=MM13\Forword
         misc_feature    4824..4840
                         /vntifkey="21"
                         /label=M13-\R\primer
         misc_feature    complement(7345..7361)
                         /vntifkey="21"
                         /label=M13-R\Primer
         rep_origin      complement(7783..8411)
                         /vntifkey="33"
                         /label=ColE1\origin
         CDS             complement(9859..9927)
                         /vntifkey="4"
                         /label=LacZ\alpha\Reporter
         misc_binding    complement(7367..7389)
                         /vntifkey="20"
                         /label=LacO
         CDS             8563..9222
                         /vntifkey="4"
                         /label=Amp\R
         CDS             1033..1368
                         /vntifkey="4"
                         /label=Rep(+)
         CDS             complement(1966..2592)
                         /vntifkey="4"
                         /label=Tet\Resistance
         misc_feature    complement(2722..3294)
                         /vntifkey="21"
                         /label=SecY-F
         misc_feature    5362..6045
                         /vntifkey="21"
                         /label=Cm\Resistance
         misc_feature    4883..5114
                         /vntifkey="21"
                         /label=attP2
         CDS             6365..6670
                         /vntifkey="4"
                         /label=ccdB
         misc_feature    complement(7066..7297)
                         /vntifkey="21"
                         /label=attP2
         misc_feature    4883..5114
                         /vntifkey="21"
                         /label=attP1
         misc_feature    complement(7066..7297)
                         /vntifkey="21"
                         /label=attP1
    BASE COUNT     3069 a      1935 c      2129 g      2897 t 
    ORIGIN
            1 ctgcagaacg gattgttgat gattacgaaa atattaagag cacagactat tacacagaaa 
           61 atcaagaatt aaaaaaacgt agagagagtt tgaaagaagt agtgaataca tggaaagagg 
          121 ggtatcacga aaaaagtaaa gaggttaata aattaaagcg agagaatgat agtttgaatg 
          181 agcagttgaa tgtatcagag aaatttcaag ctagtacagt gactttatat cgtgctgcga 
          241 gggcgaattt ccctgggttt gagaaagggt ttaataggct taaagagaaa ttctttaatg 
          301 attccaaatt tgagcgtgtg ggacagttta tggatgttgt acaggataat gtccagaagg 
          361 tcgatagaaa gcgtgagaaa cagcgtacag acgatttaga gatgtagagg tacttttatg 
          421 ccgagaaaac tttttgcgtg tgacagtcct taaaatatac ttagagcgta agcgaaagta 
          481 gtagcgacag ctattaactt tcggttgcaa agctctagga tttttaatgg acgcagcgca 
          541 tcacacgcaa aaaggaaatt ggaataaatg cgaaatttga gatgttaatt aaagaccttt 
          601 ttgaggtctt tttttcttag atttttgggg ttatttaggg gagaaaacat aggggggtac 
          661 tacgacctcc cccctaggtg tccattgtcc attgtccaaa caaataaata aatattgggt 
          721 ttttaatgtt aaaaggttgt tttttatgtt aaagtgaaaa aaacagatgt tgggaggtac 
          781 agtgatagtt gtagatagaa aagaagagaa aaaagttgct gttactttaa gacttacaac 
          841 agaagaaaat gagatattaa atagaatcaa agaaaaatat aatattagca aatcagatgc 
          901 aaccggtatt ctaataaaaa aatatgcaaa ggaggaatac ggtgcatttt aaacaaaaaa 
          961 agatagacag cactggcatg ctgcctatct atgactaaat tttgttaaat gtattagcac 
         1021 cgttattata tcatgagcga aaatgtaata aaagaaactg aaaacaagaa aaattcaaga 
         1081 ggacgtaatt ggacatttgt tttatatcca gaatcagcaa aagccgagtg gttagagtat 
         1141 ttaaaagagt tacacattca atttgtagtg tctccattac atgataggga tactgataca 
         1201 gaagatagga tgaaaaaaga gcattatcat attctagtga tgtatgaggg taataaatct 
         1261 tatgaacaga taaaaataat taacagaaga attgaatgcg actattccgc agattgcagg 
         1321 aagtgtgaaa ggtcttgtga gatatatgct tcacatggac gatcctaata aatttaaata 
         1381 tcaaaaagaa gatatgatag tttatggcgg tgtagatgtt gatgaattat taaagaaaac 
         1441 aacaacagat agatataaat taattaaaga aatgattgag tttattgatg aacaaggaat 
         1501 cgtagaattt aagagtttaa tggattatgc aatgaagttt aaatttgatg attggttccc 
         1561 gcttttatgt gataactcgg cgtatgttat tcaagaatat ataaaatcaa atcggtataa 
         1621 atctgaccga tagattttga atttaagagt gtcacaagac actctttttt cgcaccagcg 
         1681 aaaactggtt taagccgact gcgcaaaaga cataatcgat tcacaaaaaa taggcacacg 
         1741 aaaaacaagt taagggatgc agtttatgca tcccttaact tacttattaa ataatttata 
         1801 gctattgaaa agagataaga attgttcaaa gctaatattg tttaaatcgt caattcctgc 
         1861 atgttttaag gaattgttaa attgattttt tgtaaatatt ttcttgtatt ctttgttatc 
         1921 ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc 
         1981 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg 
         2041 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc 
         2101 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca 
         2161 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc 
         2221 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg 
         2281 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt 
         2341 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg 
         2401 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg 
         2461 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc 
         2521 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa 
         2581 tctagacatc attaattcct cctttttgtt gacactctat cattgataga gttatttgtc 
         2641 aaactagttt tttatttgga tcccctcgag ttcatgaaaa actaaaaaaa atattgacac 
         2701 tctatcattg atagagtata attaaaataa gcttgatggg aatatgataa tagaaatacc 
         2761 attaccaaca ccgaactgag tgatttgatc accaagccat attaagaaag cagttcctgc 
         2821 tgtcaaaact agtgctatta ataaataact cataattgac tgattgataa tcagcgcacc 
         2881 tttgagataa ttattaaatt ggaatgccat acctatagat tggataaatg ctaaagaaat 
         2941 tgctaaataa cgagtaacgt tatttaactt tcttctacct acttcacctt gttttgccca 
         3001 ttctgagaat ttagggacaa tatccatttg taataattgc attacgattg atgcagtgat 
         3061 gtagggtaca atacccattg caaaaataga aaatcgtttc aaggctccgc caccaaaagt 
         3121 atttaataac tcagtggcac cttgagaacc ttggggatta tcaaaagctg caggatttac 
         3181 tcctggagct ggtatataag tccctatttt aaaaattact aacattgcta gtgtgaagaa 
         3241 aatcttgtta cgaacctctt ttgttctaaa gaagttcaca agggtttgaa tcattagatc 
         3301 accccggggt gatctaatga ttcatatcta catcaaatgc tggggcatat gttttaccct 
         3361 caggtacagg taagtcaccc gcttcaaacg tcttcttaat atgcgtaatt gcatctactc 
         3421 taaatccatc aatgccttta tcaaaccacc agttcatcat ttcaaataca gcatctctaa 
         3481 cttccggatt accccaattc aaatcaggtt gttttttact gaataaatgg aaataatatt 
         3541 gctcagtatt agcatcatat tcccgcgcgc caattgagct cccctttcta tgtatgtttt 
         3601 ttactagtca tttaaaacga tacattaata ggtacgaaaa agcaactttt tttgcgctta 
         3661 aaaccagtca taccaataac ttaagggtaa ctagcctcgc cggcaatagt tacccttatt 
         3721 atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa aaacacaaaa 
         3781 gaccacattt tttaatgtgg tcttttattc ttcaactaaa gcacccatta gttcaacaaa 
         3841 cgaaaattgg ataaagtggg atatttttaa aatatatatt tatgttacag taatattgac 
         3901 ttttaaaaaa ggattgattc taatgaagaa agcagacaag taagcctcct aaattcactt 
         3961 tagataaaaa tttaggaggc atatcaaatg aaatttaata aaattgattt agacaattgg 
         4021 aagagaaaag agatatttaa tcattatttg aaccaacaaa cgacttttag tataaccaca 
         4081 gaaattgata ttagtgtttt ataccgaaac ataaaacaag aaggatataa attttaccct 
         4141 gcatttattt tcttagtgac aagggtgata aactcaaata cagcttttag aactggttac 
         4201 aatagcgacg gagagttagg ttattgggat aagttagagc cactttatac aatttttgat 
         4261 ggtgtatcta aaacattctc tggtatttgg actcctgtaa agaatgactt caaagagttt 
         4321 tatgatttat acctttctga tgtagagaaa tataatggtt cggggaaatt gtttcccaaa 
         4381 acacctatac ctgaaaatgc tttttctctt tctattattc catggacttc atttactggg 
         4441 tttaacttaa atatcaataa taatagtaat taccttctac ccattattac agcaggaaaa 
         4501 ttcattaata aaggtaattc aatatattta ccgctatctt tacaggtaca tcattctgtt 
         4561 tgtgatggtt atcatgcagg attgtttatg aactctattc aggaattgtc agataggcct 
         4621 aatgactggc ttttataata tgagataatg ccgactgtac tttttacagt cggttttcta 
         4681 atgtcactaa cctgccccgt tagttgaaga aggtttttat attacagctc cagatccata 
         4741 tccttctttt tctgaaccga cttctccttt ttcgcttctt tattccaatt gctttattga 
         4801 cgttgagcct cggaaccggt acccaggaaa cagctatgac catgtaatac gactcactat 
         4861 acggggatat cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg 
         4921 ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctgaa 
         4981 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag 
         5041 actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt 
         5101 attagtgacc tgtagtcgac taagttggca gcatcacccg acgcactttg cgccgaataa 
         5161 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc 
         5221 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc 
         5281 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt 
         5341 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 
         5401 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 
         5461 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 
         5521 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 
         5581 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 
         5641 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 
         5701 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 
         5761 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 
         5821 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 
         5881 ggcaaatatt atacgcaagg cgacaaggtg ctgatgacgc tggcgattca ggttcatcat 
         5941 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 
         6001 gagtggcagg gggggcgtaa acgccgcgtg gatccggctt actaaaagcc agataacagt 
         6061 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa 
         6121 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag 
         6181 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg 
         6241 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg 
         6301 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg 
         6361 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga 
         6421 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagcgc 
         6481 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga 
         6541 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga 
         6601 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg 
         6661 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgat acagtagaaa 
         6721 ttacagaaac tttatcacgt ttagtaagta tagaggctga aaatccagat gaagccgcac 
         6781 gacttgtaag agaaaagtat aagagttgtg aaattgttct tgatgcagat gattttcagg 
         6841 actatgacac tagcgtatat gaataggtag atgtttttat tttgtcacac aaaaaagagg 
         6901 ctcgcacctc tttttcttat ttctttttat gatttaatac ggcattgagg acaatagcga 
         6961 gtaggctgga tacgacgatt ccgtttgaga agaacatttg gaaggctgtc ggtcgactaa 
         7021 gttggcagca tcacccgaag aacatttgga aggctgtcgg tcgactacag gtcactaata 
         7081 ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta ttatgtagtc 
         7141 tgttttttat gcaaaatcta atttaatata ttgatattta tatcacttta cgtttctcgt 
         7201 tcagcttttt tgtacaaagt tggcattata aaaaagcatt gctcatcaat ttgttgcaac 
         7261 gaacaggtca ctatcagtca aaataaaatc attatttggg gcccgagctt aagactggcc 
         7321 gtcgttttac gaattcgtga ctatgtcata gctgtttcct gtgtgaaatt gttatccgct 
         7381 cacaattcca cacaacatac gagccggaag cataaagtga aagcctgggg tgcctaaaat 
         7441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
         7501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
         7561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
         7621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
         7681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
         7741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
         7801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
         7861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
         7921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
         7981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
         8041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
         8101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
         8161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
         8221 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
         8281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
         8341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
         8401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
         8461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
         8521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
         8581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
         8641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
         8701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
         8761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
         8821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
         8881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
         8941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
         9001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
         9061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
         9121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
         9181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
         9241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
         9301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
         9361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
         9421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
         9481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
         9541 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 
         9601 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 
         9661 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 
         9721 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 
         9781 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
         9841 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 
         9901 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 
         9961 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 
        10021 gcttgcatgc 
    //
    

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