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  • pGL4.24[luc2P/minP]

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Promega
    載體名稱: pGL4.24[luc2P/minP]
    質粒類型: 熒光素酶載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: --
    啟動子: --
    載體大小: 4411 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: --
    宿主細胞(系): --
    備注: 用不穩定熒光素酶測定轉錄反應元件活性的最小啟動子載體。
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    載體質粒圖譜和多克隆位點信息

    pGL4.24[luc2PminP]載體圖譜

    載體簡介

    載體序列

    LOCUS       pGL4.24[luc2P minP]                4411 bp ds-DNA     circular SYN 29-JUN-2013
    DEFINITION  Vector with a minimal promoter for measuring the activity of 
                transcriptional response elements using destabilized luciferase.
    ACCESSION   DQ904456
    VERSION     .
    KEYWORDS    pGL4.24[luc2P minP] 
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 4411)
      AUTHORS   Promega
      TITLE     Direct Submission
      JOURNAL   Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..4411
                         /organism="synthetic DNA construct"
                         /lab_host="Mammalian Cells"
                         /mol_type="other DNA"
         misc_feature    1..70
                         /note="MCS"
                         /note="multiple cloning site"
         promoter        77..108
                         /note="minP"
                         /note="minimal TATA-box promoter with low basal activity"
         CDS             141..1790
                         /codon_start=1
                         /product="firefly luciferase"
                         /note="luciferase"
                         /note="synthetic luc2 version of the luciferase gene"
                         /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                         HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                         ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                         MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                         RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                         IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                         GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                         GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                         LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                         FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
         CDS             1794..1913
                         /codon_start=1
                         /product="PEST degradation sequence from mouse ornithine 
                         decarboxylase"
                         /note="hPEST"
                         /note="human codon-optimized"
                         /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
         polyA_signal    1965..2086
                         /note="SV40 poly(A) signal"
                         /note="SV40 polyadenylation signal"
         rep_origin      complement(2505..3093)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(3293..4153)
                         /codon_start=1
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         polyA_signal    4258..4306
                         /note="synthetic polyadenylation signal"
         misc_feature    4320..4411
                         /note="pause site"
                         /note="RNA polymerase II transcriptional pause signal from 
                         the human alpha-2 globin gene"
    ORIGIN
            1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
           61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
          121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
          181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
          241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
          301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
          361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
          421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
          481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
          541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
          601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
          661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
          721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
          781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
          841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
          901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
          961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
         1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
         1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
         1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
         1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
         1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
         1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
         1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
         1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
         1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
         1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
         1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
         1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
         1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
         1801 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
         1861 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
         1921 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
         1981 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
         2041 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
         2101 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
         2161 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
         2221 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
         2281 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
         2341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
         2401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
         2461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
         2521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
         2581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
         2641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
         2701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
         2761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
         2821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
         2881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
         2941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
         3001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
         3061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
         3121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
         3181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
         3241 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
         3301 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
         3361 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
         3421 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
         3481 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
         3541 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
         3601 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
         3661 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
         3721 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
         3781 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
         3841 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
         3901 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
         3961 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
         4021 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
         4081 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
         4141 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
         4201 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
         4261 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
         4321 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
         4381 agtgcaagtg caggtgccag aacatttctc t
    //
    
    
    

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