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  • pGL2-Basic

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pGL2Basic載體基本信息

    出品公司: Promega
    載體名稱: pGL2-Basic
    質粒類型: 熒光素酶載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: --
    啟動子: --
    載體大小: 5598 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: --
    宿主細胞(系): --
    備注:
    pGL2-Basic沒有增強子或啟動子。宿主:大腸桿菌JM109,哺乳動物。相關載體:f1,pBR322,pGL2-Enhancer, pGL2-Promoter,pGL2-Control。(信息來源:VectorDB(http://seq.yeastgome.org/VectorDB.))無啟動子載體,用于用熒光素酶測定測定啟動子和增強子序列的活性。
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    pGL2Basic載體質粒圖譜和多克隆位點信息

    pGL2-Basic載體圖譜

    pGL2Basic載體簡介

    pGL2Basic載體序列

    LOCUS       pGL2-Basic                5598 bp ds-DNA     circular SYN 28-JUN-2013
    DEFINITION  Promoterless vector for measuring the activity of promoter and 
                enhancer sequences with a luciferase assay.
    ACCESSION   X65323
    VERSION     .
    KEYWORDS    pGL2-Basic
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 5598)
      AUTHORS   Promega
      TITLE     Direct Submission
      JOURNAL   Exported Monday, February 3, 2020 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..5598
                         /organism="synthetic DNA construct"
                         /lab_host="Mammalian Cells"
                         /mol_type="other DNA"
         misc_feature    1..52
                         /note="MCS"
                         /note="multiple cloning site"
         CDS             76..1728
                         /codon_start=1
                         /gene="luc"
                         /product="firefly luciferase"
                         /note="luciferase"
                         /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                         HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                         ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                         MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                         RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                         IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                         GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                         GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                         LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                         FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
         intron          1969..2034
                         /note="small t intron"
                         /note="simian virus 40 (SV40) small t antigen intron"
         polyA_signal    2609..2743
                         /note="SV40 poly(A) signal"
                         /note="SV40 polyadenylation signal"
         rep_origin      complement(3056..3644)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(3815..4675)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(4676..4780)
                         /gene="bla"
                         /note="AmpR promoter"
         rep_origin      4807..5262
                         /direction=RIGHT
                         /note="f1 ori"
                         /note="f1 bacteriophage origin of replication; arrow 
                         indicates direction of (+) strand synthesis"
         polyA_signal    5452..5573
                         /note="SV40 poly(A) signal"
                         /note="SV40 polyadenylation signal"
    ORIGIN
            1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttggcattcc
           61 ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
          121 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
          181 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
          241 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
          301 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
          361 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
          421 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
          481 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
          541 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
          601 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
          661 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
          721 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
          781 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
          841 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
          901 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
          961 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
         1021 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
         1081 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
         1141 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
         1201 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
         1261 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
         1321 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
         1381 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
         1441 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
         1501 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
         1561 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
         1621 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
         1681 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
         1741 ttcagcgatg acgaaattct tagctattgt aatactgcga tgagtggcag ggcggggcgt
         1801 aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg
         1861 ataataagcg gatgaatggc agaaattcgc cggatctttg tgaaggaacc ttacttctgt
         1921 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
         1981 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
         2041 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
         2101 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
         2161 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
         2221 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
         2281 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
         2341 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
         2401 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
         2461 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
         2521 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
         2581 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
         2641 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
         2701 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtcgac cgatgccctt
         2761 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
         2821 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
         2881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
         2941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
         3001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
         3061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
         3121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
         3181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
         3241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
         3301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
         3361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
         3421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
         3481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
         3541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
         3601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
         3661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
         3721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
         3781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
         3841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
         3901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
         3961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
         4021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
         4081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
         4141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
         4201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
         4261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
         4321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
         4381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
         4441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
         4501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
         4561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
         4621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
         4681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
         4741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
         4801 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
         4861 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
         4921 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
         4981 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
         5041 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
         5101 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
         5161 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
         5221 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
         5281 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
         5341 taccatgata agtaagtaat attaaggtac gtggaggttt tacttgcttt aaaaaacctc
         5401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
         5461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
         5521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatggtact
         5581 gtaactgagc taacataa
    //
    
    
    

    pGL2Basic載體質粒圖譜pdf版和相關資料下載

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