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  • pSilencer5.1-U6 Retro

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen/ Ambion
    載體名稱: pSilencer5.1-U6 Retro, pSilencer 5.1-U6 Retro, pSilencer5.1-U6-Retro
    質粒類型: 哺乳動物細胞逆病毒基因沉默載體
    高拷貝/低拷貝: 低拷貝
    啟動子: U6
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 6486bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐
    篩選標記: 嘌呤霉素(Puromycin)
    備注: 逆病毒基因沉默載體,能夠將發卡的siRNA轉染進入大多數哺乳細胞中。
    產品目錄號: AM5784
    穩定性: 穩表達
    組成型: 組成型
    病毒/非病毒: 逆病毒

    載體質粒圖譜和多克隆位點信息

    pSilencer5.1-U6 Retro載體圖譜



    pSilencer5.1-U6 Retro多克隆位點

    載體簡介

    載體序列

    LOCUS       pSilencer\5.1-U6 Retro       6486 bp    DNA     circular      6-OCT-2013
    SOURCE      
      ORGANISM  
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTNAME|pSilencer 5.1-U6 Retro|
    BASE COUNT     1460 a      1840 c      1693 g      1493 t 
    ORIGIN
            1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
           61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
          121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
          181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
          241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
          301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
          361 tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc 
          421 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 
          481 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 
          541 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt 
          601 ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca 
          661 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca 
          721 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc 
          781 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga 
          841 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 
          901 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga 
          961 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac 
         1021 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa 
         1081 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac 
         1141 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg 
         1201 ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcattt ggaggttcca 
         1261 ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag gtaagctggc 
         1321 cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt 
         1381 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac 
         1441 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg 
         1501 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt 
         1561 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc 
         1621 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg 
         1681 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc 
         1741 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt 
         1801 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt 
         1861 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt 
         1921 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc 
         1981 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc 
         2041 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc 
         2101 ctccctttat ccagccctca ctccttctct aggcgccgga attagatcga tctctcgagg 
         2161 tcgacggtat cgataagctt ttccaaaaaa gtactgctta cgatacggtc tcttgaaccg 
         2221 tatcgtaagc agtactggat cccgcgtcct ttccacaaga tatataaacc caagaaatcg 
         2281 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact 
         2341 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg 
         2401 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat 
         2461 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg 
         2521 gtcacgctcc gtcacgtggt gcgttttgcc tgcgcgtctt tccactgggg aattctaccg 
         2581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 
         2641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 
         2701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 
         2761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 
         2821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 
         2881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 
         2941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 
         3001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 
         3061 ctcatctccg ggcctttcga cctgcagccc aagctagctt accatgaccg agtacaagcc 
         3121 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 
         3181 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 
         3241 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 
         3301 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 
         3361 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 
         3421 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 
         3481 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 
         3541 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 
         3601 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 
         3661 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc 
         3721 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt 
         3781 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagagaa 
         3841 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact 
         3901 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa 
         3961 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc 
         4021 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg 
         4081 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc 
         4141 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa 
         4201 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc 
         4261 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac 
         4321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
         4381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
         4441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
         4501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
         4561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
         4621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
         4681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
         4741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
         4801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
         4861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
         4921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
         4981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
         5041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
         5101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
         5161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
         5221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
         5281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
         5341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
         5401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
         5461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
         5521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
         5581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
         5641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
         5701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
         5761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
         5821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
         5881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
         5941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
         6001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
         6061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
         6121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
         6181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
         6241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
         6301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
         6361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
         6421 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 
         6481 ttcgtc 
    //
    

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