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  • pMSCVneo

    價格:1200元

    聯系方式:I47-825O-882O

    買家導航

    pMSCV-Neo載體基本信息

    出品公司: Clontech
    載體名稱: pMSCVneo, pMSCV-neo, pMSCV neo
    質粒類型: 哺乳動物細胞逆病毒基因表達載體
    高拷貝/低拷貝: 低拷貝
    啟動子: LTR
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 6436bp
    5' 測序引物及序列: MSCV-F: CCCTTGAACCTCCTCGTTCGACC
    3' 測序引物及序列: pMSCV-R: GAGACGTGCTACTTCCATTTGTC
    載體標簽: --
    載體抗性: 氨芐
    篩選標記: 新霉素(Neomycin)
    備注: 逆病毒表達載體,優化后,有利于在造血干細胞中和癌細胞中進行基因表達。
    產品目錄號: 634401
    穩定性: 穩表達
    組成型: 組成型
    病毒/非病毒: 逆病毒

    pMSCV-Neo載體質粒圖譜和多克隆位點信息

    pMSCVneo載體圖譜



    pMSCVneo多克隆位點

    RNAi-Ready pSIREN-RetroQ載體特征

    pMSCV-Neo載體簡介

    pMSCV-Neo載體序列

    LOCUS       pMSCVneo   6436 bp    DNA   circular             4-DEC-2009
    COMMENT     Created by Clontech Laboratories Inc. 
    COMMENT     Cat. No. 634401,631461
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         misc_signal     516..1405
                         /label=Extended\packaging\signal
         promoter        1429..1937
                         /label=Phosphoglycerate\Kinase\Promoter
         CDS             1951..2751
                         /label=Neo(R)
         LTR             2859..3342
                         /label=3'\PCMV\LTR
         LTR             1..515
                         /label=5'\MMSV\LTR
         CDS             complement(4645..5502)
                         /label=Amp(R)
         rep_origin      3882..3882
                         /label=pUC\origin
         splicing_signal 519..522
                         /label=Splice\Donor
         splicing_signal 1397..1399
                         /label=Splice\Acceptor
    BASE COUNT     1458 a      1758 c      1708 g      1512 t 
    ORIGIN
            1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
           61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
          121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
          181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
          241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
          301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
          361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
          421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
          481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
          541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
          601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
          661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
          721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
          781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
          841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
          901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
          961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
         1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
         1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
         1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
         1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
         1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
         1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
         1381 ccctcactcc ttctctaggc gccggaattc gttaacctcg agagatctaa ttctaccggg 
         1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca 
         1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc 
         1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga 
         1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc 
         1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg 
         1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg 
         1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg 
         1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct 
         1921 catctccggg cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg 
         1981 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 
         2041 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 
         2101 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 
         2161 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 
         2221 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 
         2281 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 
         2341 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 
         2401 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 
         2461 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 
         2521 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 
         2581 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 
         2641 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 
         2701 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat 
         2761 ccgtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa 
         2821 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt 
         2881 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga 
         2941 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 
         3001 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat 
         3061 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc 
         3121 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc 
         3181 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg 
         3241 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg 
         3301 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga 
         3361 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag 
         3421 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag 
         3481 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc 
         3541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
         3601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
         3661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
         3721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
         3781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
         3841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
         3901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
         3961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
         4021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
         4081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
         4141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
         4201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
         4261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
         4321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
         4381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
         4441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
         4501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
         4561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
         4621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
         4681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
         4741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
         4801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
         4861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
         4921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
         4981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
         5041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
         5101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
         5161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
         5221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
         5281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
         5341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
         5401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
         5461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
         5521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
         5581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 
         5641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
         5701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 
         5761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 
         5821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 
         5881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 
         5941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
         6001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
         6061 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg 
         6121 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 
         6181 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 
         6241 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta 
         6301 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat 
         6361 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc 
         6421 cagaaaaagg ggggaa 
    //
    

    pMSCV-Neo載體質粒圖譜pdf版和相關資料下載

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