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  • pFB

    價格:3000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Agilent
    載體名稱: pFB
    質粒類型: 逆病毒載體
    高拷貝/低拷貝: 低拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: --
    載體大小: 5117 bp
    5' 測序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記:
    克隆菌株: DH5α 等通用菌株
    宿主細胞(系): 常規細胞系(293、CV-1、CHO等)
    備注: --
    產品目錄號: #217563
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 逆轉錄病毒

    載體質粒圖譜和多克隆位點信息

    pFB載體圖譜



    pFB 多克隆位點

    pFB 載體特征

    載體簡介

    pFB載體描述
    
    The Agilent pFB and pFB-Neo plasmid vectors for retroviral gene delivery and expression are derived from the Moloney murine leukemia virus (MMLV) and can be used to produce high titer viral stocks with MMLVbased packaging cell lines.1,2 Both vectors contain the bacterial origin of replication and ampicillin-resistance gene from pBR322, an extended MMLV packaging signal (ψ+), and a multiple cloning site (MCS) located between the MMLV 5′ and 3′ long terminal repeat sequences (LTRs).
    The pFB vector is a minimal MMLV-based vector that can accommodate larger inserts than pFB-Neo. The pFB vector does not contain any exogenous sequence other than the sequence of the MCS.
    The pFB-Neo vector differs from the pFB vector only by the presence of a cassette consisting of the internal ribosome entry site (IRES) from the encephalomyocarditis virus (EMCV) and the neomycin-resistance gene (neor). The open reading frame (ORF) for the neor gene is positioned downstream of the MCS and follows the IRES. A dicistronic message encoding the gene of interest and the neor gene is expressed from the viral promoter within the 5′ LTR.
    
    
    
    pFB載體限制性酶切位點
    
    pFB, 5117 bp			             version 013001
    
    
    Enzymes with 1-10 cleavage sites:
               
                #sites   --  Bp position of recognition site --
    
      AatII          2      978,   5043
      Acc65I         3      647,   1846,   2643
      AccI           3     1967,   2057,   3003
      AflII          3      202,   1265,   2198
      AflIII         2      164,   3232
      AgeI           1     1840
      AhdI           6      687,    733,   1274,   2683,   2729
                           4120
      Alw44I         4     1054,   3048,   3546,   4792
      AlwNI          5      323,    398,   2319,   2394,   3643
      ApaI           1     1238
      ApoI           3       87,   1128,   2063
      AvaI           9      577,    610,    643,   1241,   2018
                           2074,   2573,   2606,   2639
      BamHI          1     2069
      BanII          7      580,    593,    764,   1238,   2576
                           2589,   2760
      BbeI           3      615,   1656,   2611
      BbsI           1     5110
      BbvCI          2      453,   2449
      BciVI          5      656,   1986,   2652,   3441,   4968
      BglI           1     4239
      BglII          1     1678
      BpmI           3     1799,   2146,   4210
      Bpu10I         7      337,    412,    453,   1548,   2333
                           2408,   2449
      BsaAI          3     1816,   2000,   2984
      BsaHI          6      615,    978,   1656,   2611,   4661
                           5043
      BsaI           9      694,    715,    782,   1414,   1802
                           2690,   2711,   2778,   4192
      BsaWI          5      138,   1840,   3438,   3585,   4416
      BseRI          5      718,   1068,   1561,   1600,   2714
      BsiEI          8      961,   1034,   1838,   2080,   3145
                           3569,   4492,   4641
      BsiHKAI        7      580,   1054,   2576,   3048,   3546
                           4707,   4792
      BsmBI          6      976,   1093,   1337,   1396,   1582
                           2881
      Bsp120I        1     1238
      BspHI          3     3952,   4960,   5065
      BspLU11I       2      164,   3232
      BsrBI          5     1299,   2077,   2102,   3163,   4964
      BsrDI          2     4179,   4361
      BsrGI          1     1541
      BssHII         2      563,   2559
      BssSI          3     3405,   4789,   5096
      Bst1107I       1     3003
      BstAPI         5      322,    397,   2318,   2393,   3050
      BstEII         1     1346
      BstF5I         9      682,   1386,   1573,   2031,   2678
                           2907,   4105,   4286,   4573
      Bsu36I         1     1276
      Cfr10I         2     1840,   4205
      Csp6I          8      648,    895,   1542,   1819,   1847
                           2644,   3039,   4604
      DraI           3     3989,   4008,   4700
      DraIII         1     1873
      DrdI           2     2921,   3334
      DsaI           2      151,    931
      EaeI           7      827,    961,   1368,   1389,   1668
                           2080,   4513
      EarI           4     1334,   1564,   3110,   4914
      Ecl136II       2      580,   2576
      Eco52I         2      961,   2080
      Eco57I         2     3759,   4807
      Eco72I         1     2000
      EcoNI          1     1647
      EcoO109I       5      499,   1477,   1924,   2495,   5100
      EcoRI          1     2063
      EcoRV          4      308,    383,   2304,   2379
      EheI           3      615,   1656,   2611
      FokI           9      682,   1386,   1573,   2031,   2678
                           2907,   4105,   4286,   4573
      FspI           1     4345
      HaeII          5      615,   1656,   2611,   3106,   3476
      HgaI           6      889,   1166,   2938,   3334,   3912
                           4662
      HincII         2     2057,   4664
      HinfI         10      637,   1020,   1045,   1863,   2108
                           2633,   3132,   3207,   3603,   4120
      KasI           3      615,   1656,   2611
      KpnI           3      647,   1846,   2643
      MaeII         10      979,   1340,   1381,   1817,   2001
                           2985,   3935,   4351,   4724,   5044
      MboII         10     1334,   1565,   2096,   3111,   3882
                           3973,   4728,   4806,   4915,   5111
      MscI           3      827,   1368,   1668
      MslI           4      159,   4373,   4532,   4891
      MunI           2       11,     21
      NarI           3      615,   1656,   2611
      NdeI           3     1664,   1672,   3054
      NheI           5        6,     16,     26,    197,   2193
      NotI           1     2079
      NspI           3      164,   2867,   3232
      PleI           9      637,   1020,   1045,   1863,   2108
                           2633,   3132,   3603,   4120
      PpuMI          4      499,   1477,   1924,   2495
      PshAI          1     1015
      Psp1406I       2     4350,   4723
      PstI           3     1178,   1360,   4366
      PvuI           2     1034,   4492
      PvuII          4      286,    361,   2282,   2357
      RsaI           8      648,    895,   1542,   1819,   1847
                           2644,   3039,   4604
      SacI           2      580,   2576
      SacII          1      151
      SalI           1     2057
      SapI           1     3109
      ScaI           1     4603
      SchI           9      637,   1020,   1045,   1863,   2108
                           2633,   3132,   3603,   4120
      SexAI          1     1474
      SfcI           7      182,   1178,   1360,   2178,   3497
                           3688,   4366
      SmaI           4      643,   1241,   2018,   2639
      SmlI           8      202,   1265,   2074,   2198,   3338
                           3600,   3877,   4745
      SpeI           1      897
      SrfI           1     1240
      SspI           1     4927
      StyI           5      496,    706,   1504,   2492,   2702
      TaiI          10      979,   1340,   1381,   1817,   2001
                           2985,   3935,   4351,   4724,   5044
      TaqI           9     1297,   1608,   1619,   2050,   2058
                           2075,   2119,   3332,   4776
      TatI           3     1541,   3038,   4603
      TfiI           1     3207
      Tsp45I         6     1282,   1491,   2887,   2982,   4382
                           4593
      TspRI         10     1284,   1875,   3128,   3634,   3647
                           3918,   4067,   4172,   4519,   4546
      Tth111I        4      633,   1473,   2629,   2976
      VspI           1     4296
      XbaI           3      464,   2036,   2460
      XhoI           1     2074
      XhoII          8     1678,   2069,   3873,   3884,   3970
                           3982,   4750,   4767
      XmaI           4      643,   1241,   2018,   2639
      XmnI           1     4720
    
    
    
    
    
    Enzymes that do NOT cut molecule:
    
    AccIII      AscI        BclI        BlnI        Bpu1102I    
    BsaBI       BsgI        BsiWI       BsmI        BspMI       
    BstBI       BstXI       ClaI        Eco47III    FseI        
    HindIII     HpaI        MluI        NaeI        NcoI        
    NgoMIV      NruI        NsiI        PacI        PmeI        
    Ppu10I      RsrII       SanDI       SbfI        SfiI        
    SgfI        SgrAI       SnaBI       SphI        StuI        
    SwaI        Van91I      XcmI        
    
    
    

    載體序列

    pFB, 5117 bp                                  version 013001
    
    NOTE: The following sequence has been verified for accuracy
    at the junctions. The remainder of the sequence has been 
    obtained from existing data. 
    
       1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG  
      51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT  
     101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG  
     151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA  
     201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT  
     251  AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC  
     301  CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA  
     351  ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG  
     401  TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA  
     451  GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG  
     501  GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC  
     551  TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC  
     601  AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA  
     651  CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG  
     701  CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG  
     751  GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC  
     801  CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG  
     851  TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA  
     901  GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT  
     951  TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC  
    1001  CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT  
    1051  TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG  
    1101  AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA  
    1151  CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC  
    1201  TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG  
    1251  ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA  
    1301  GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA  
    1351  CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC  
    1401  GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC  
    1451  ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG  
    1501  AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT  
    1551  AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC  
    1601  TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT  
    1651  CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG  
    1701  CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC  
    1751  CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT  
    1801  GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC  
    1851  CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT  
    1901  AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC  
    1951  CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC  
    2001  ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT  
    2051  CGAATTGTCG ACGAATTCGG ATCCTCGAGC GGCCGCGATC CGAGTTCTTC  
    2101  TGAGCGGGAC TCTGGGGTTC GATAAAATAA AAGATTTTAT TTAGTCTCCA  
    2151  GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT  
    2201  AAGTAACGCC ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAG  
    2251  AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA  
    2301  CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG  
    2351  ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC  
    2401  TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC  
    2451  TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC  
    2501  TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC  
    2551  TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC  
    2601  CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG  
    2651  TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT  
    2701  TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT  
    2751  TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC  
    2801  GACCCACCAC CGGGAGGTAA GCTGGCTGCC TCGCGCGTTT CGGTGATGAC  
    2851  GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT  
    2901  GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG  
    2951  TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA  
    3001  GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG  
    3051  CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG  
    3101  CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG  
    3151  TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA  
    3201  TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA  
    3251  GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA  
    3301  GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG  
    3351  TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG  
    3401  CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT  
    3451  CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT  
    3501  AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA  
    3551  CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC  
    3601  TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT  
    3651  GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT  
    3701  GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT  
    3751  GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA  
    3801  TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA  
    3851  GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT  
    3901  CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG  
    3951  GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA  
    4001  ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA  
    4051  GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT  
    4101  CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG  
    4151  GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC  
    4201  GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC  
    4251  GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA  
    4301  TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA  
    4351  ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT  
    4401  ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC  
    4451  CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG  
    4501  TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG  
    4551  CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG  
    4601  TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT  
    4651  GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT  
    4701  TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG  
    4751  GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA  
    4801  ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA  
    4851  ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG  
    4901  TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG  
    4951  GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA  
    5001  CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA  
    5051  AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA  
    5101  GGCCCTTTCG TCTTCAA
    

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