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  • pBABE-neo-SV40LT

    價格:3800元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Bob Weinberg
    載體名稱: pBABE-neo-SV40LT
    質粒類型: 逆病毒載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: MSCV
    載體大小: 7464 bp
    5' 測序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
    3' 測序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
    載體標簽:
    載體抗性: 氨芐青霉素
    篩選標記: Neomycin
    克隆菌株: DH5α、HB101等
    宿主細胞(系): 常規哺乳動物細胞系
    備注:
    逆病毒載體pBABE-neo-SV40LT表達Large T Antigen,用來建永生細胞系。
    推薦使用逆病毒包裝載體:pUMVC、pCMV-VSV-G 。
     
    產品目錄號: #1780
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 逆轉錄病毒

    載體質粒圖譜和多克隆位點信息

    pBABE-neo-SV40LT載體圖譜

    載體簡介

    載體序列

    LOCUS       Exported                7464 bp ds-DNA     circular SYN 07-AUG-2016
    DEFINITION  synthetic circular DNA.
    KEYWORDS    pBABE-neo SV40LT
    SOURCE      synthetic DNA construct
    REFERENCE   1  (bases 1 to 7464)
      AUTHORS   Biofeng Lab.
    FEATURES             Location/Qualifiers
         source          1..7464
                         /organism="synthetic DNA construct"
                         /mol_type="other DNA"
         LTR             10..477
                         /note="5'LTR2"
                         /note="long terminal repeat from Moloney murine leukemia 
                         virus"
         misc_feature    16..477
                         /note="3MoMuLV LTR"
         misc_feature    540..739
                         /note="MMLV Psi"
                         /note="packaging signal of Moloney murine leukemia virus 
                         (MMLV)"
         misc_feature    547..1356
                         /note="psi plus pack"
         CDS             940..1356
                         /note="gag"
                         /note="truncated Moloney murine leukemia virus (MMLV) gag 
                         gene lacking the start codon"
         CDS             1394..3520
                         /codon_start=1
                         /note="ORF frame 1"
                         /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                         GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                         CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                         TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                         CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                         CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                         VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                         KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                         FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                         TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                         LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                         DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                         CFKKPPTPPPEPET"
         misc_feature    1652..3520
                         /note="SV40LT"
                         /note="Used to create immortalized cell lines"
         misc_feature    1769..1789
                         /product="nuclear localization signal of SV40 large T 
                         antigen"
                         /note="SV40 NLS"
         misc_feature    1775..1789
                         /note="NLS"
         promoter        3606..3935
                         /note="SV40 promoter"
                         /note="SV40 enhancer and early promoter"
         enhancer        3607..3822
                         /note="SV40 enhancer"
         rep_origin      3786..3921
                         /note="SV40 ori"
                         /note="SV40 origin of replication"
         promoter        4155..4203
                         /note="Neo/Kan promoter"
         CDS             4292..5086
                         /codon_start=1
                         /gene="aph(3')-II (or nptII)"
                         /product="aminoglycoside phosphotransferase from Tn5"
                         /note="NeoR/KanR"
                         /note="confers resistance to neomycin, kanamycin, and G418 
                         (Geneticin(R))"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                         VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                         SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                         GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                         LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
         LTR             5890..6359
                         /note="long terminal repeat from Moloney murine leukemia 
                         virus"
         CDS             complement(6474..7334)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(7335..7439)
                         /gene="bla"
                         /note="AmpR promoter"
    ORIGIN
            1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
           61 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
          121 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
          181 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
          241 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
          301 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
          361 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
          421 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
          481 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
          541 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
          601 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
          661 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
          721 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
          781 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
          841 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
          901 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
          961 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
         1021 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
         1081 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
         1141 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
         1201 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
         1261 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
         1321 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccgcg accggtcgac
         1381 cagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
         1441 tctaggtctt gaaaggagtg cctgggggaa tattcctctg atgagaaagg catatttaaa
         1501 aaaatgcaag gagtttcatc ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa
         1561 tactctgtac aagaaaatgg aagatggagt aaaatatgct catcaacctg actttggagg
         1621 cttctgggat gcaactgaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
         1681 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
         1741 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
         1801 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
         1861 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
         1921 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
         1981 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
         2041 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
         2101 gactagagat ccattttctg ttattgagga aagtttgcca ggtgggttaa aggagcatga
         2161 ttttaatcca gaagaagcag aggaaactaa acaagtgtcc tggaagcttg taacagagta
         2221 tgcaatggaa acaaaatgtg atgatgtgtt gttattgctt gggatgtact tggaatttca
         2281 gtacagtttt gaaatgtgtt taaaatgtat taaaaaagaa cagcccagcc actataagta
         2341 ccatgaaaag cattatgcaa atgctgctat atttgctgac agcaaaaacc aaaaaaccat
         2401 atgccaacag gctgttgata ctgttttagc taaaaagcgg gttgatagcc tacaattaac
         2461 tagagaacaa atgttaacaa acagatttaa tgatcttttg gataggatgg atataatgtt
         2521 tggttctaca ggctctgctg acatagaaga atggatggct ggagttgctt ggctacactg
         2581 tttgttgccc aaaatggatt cagtggtgta tgacttttta aaatgcatgg tgtacaacat
         2641 tcctaaaaaa agatactggc tgtttaaagg accaattgat agtggtaaaa ctacattagc
         2701 agctgctttg cttgaattat gtggggggaa agctttaaat gttaatttgc ccttggacag
         2761 gctgaacttt gagctaggag tagctattga ccagttttta gtagtttttg aggatgtaaa
         2821 gggcactgga ggggagtcca gagatttgcc ttcaggtcag ggaattaata acctggacaa
         2881 tttaagggat tatttggatg gcagtgttaa ggtaaactta gaaaagaaac acctaaataa
         2941 aagaactcaa atatttcccc ctggaatagt caccatgaat gagtacagtg tgcctaaaac
         3001 actgcaggcc agatttgtaa aacaaataga ttttaggccc aaagattatt taaagcattg
         3061 cctggaacgc agtgagtttt tgttagaaaa gagaataatt caaagtggca ttgctttgct
         3121 tcttatgtta atttggtaca gacctgtggc tgagtttgct caaagtattc agagcagaat
         3181 tgtggagtgg aaagagagat tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa
         3241 gtttaatgtg gctatgggaa ttggagtttt agattggcta agaaacagtg atgatgatga
         3301 tgaagacagc caggaaaatg ctgataaaaa tgaagatggt ggggagaaga acatggaaga
         3361 ctcagggcat gaaacaggca ttgattcaca gtcccaaggc tcatttcagg cccctcagtc
         3421 ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
         3481 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
         3541 ttaacgggga tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg tcgaccctgt
         3601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
         3661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
         3721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
         3781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
         3841 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
         3901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcacgctgcc gcaagcactc
         3961 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
         4021 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
         4081 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
         4141 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
         4201 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
         4261 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
         4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
         4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
         4441 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
         4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
         4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
         4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
         4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
         4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
         4801 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
         4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
         4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
         4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
         5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
         5101 ttcgcatcga tgcatcgatg cggcgggtgt ggtggttacg cgtaatctgc tgcttgcaaa
         5161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
         5221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
         5281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
         5341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
         5401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
         5461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
         5521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
         5581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
         5641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
         5701 tatggaaaaa cgccagcaac gcatcgataa aataaaagat tttatttagt ctccagaaaa
         5761 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
         5821 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
         5881 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
         5941 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
         6001 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
         6061 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
         6121 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
         6181 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
         6241 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
         6301 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
         6361 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
         6421 tagtgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
         6481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
         6541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
         6601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
         6661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
         6721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
         6781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
         6841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
         6901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
         6961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
         7021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
         7081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
         7141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
         7201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
         7261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
         7321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
         7381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
         7441 acatttcccc gaaaagtgcc acct
    //
    
    

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