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  • pWPXL

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: --
    載體名稱: pWPXL
    質粒類型: 哺乳動物細胞慢病毒表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: EF-1 alpha
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 10510 bp
    5' 測序引物及序列: EF-1a Forward:   TCAAGCCTCAGACAGTGGTTC
    3' 測序引物及序列: EGFP-N: CGTCGCCGTCCAGCTCGACCAG
    載體標簽: C-EGFP
    載體抗性: 氨芐
    備注: pWPXL載體是用來進行穩定型轉基因表達。pWPXL載體含有EF-1alpha啟動子和內含子,這使得基因能夠高效表達。載體3‘ LTR區的LoxP被克隆至目的細胞的5’ LTR反轉錄區。這 使得之前在Cre酶作用下插入的基因得以移除出去。載體使用Stbl3感受態細胞擴增質粒,可以減少載體基因重組。
    產品目錄號: --
    穩定性: /
    組成型: --
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pWPXL載體圖譜


    載體簡介

    載體序列

    LOCUS       pWPXL	10510 bp 	DNA     circular	SYN
    DEFINITION  pWPXL
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..10510
                         /organism="pWPXL"
                         /mol_type="other DNA"
         misc_feature    455..635
                         /label="truncHIV-1_3_LTR"
         misc_feature    455..635
                         /label="HIV-1_5_LTR"
         misc_feature    746..790
                         /label="HIV-1_psi_pack"
         CDS             1178..2035
                         /label="ORF frame 2"
         misc_feature    1300..1533
                         /label="RRE"
         CDS             complement(1997..3037)
                         /label="ORF frame 1"
         promoter        2127..3297
                         /label="EF1a_promoter"
         misc_feature    3245..3265
                         /label="EF1a_fwd_primer"
         misc_feature    3349..3364
                         /label="cPPT"
         CDS             3544..4281
                         /label="ORF frame 1"
         gene            3547..4260
                         /label="EGFP"
                         /gene="EGFP"
         misc_feature    complement(3589..3610)
                         /label="EGFP_N_primer"
         misc_feature    3727..3756
                         /label="Y66 (EGFP)"
         misc_feature    4197..4218
                         /label="EGFP_C_primer"
         misc_feature    4342..4929
                         /label="WPRE"
         CDS             complement(4427..4930)
                         /label="ORF frame 1"
         CDS             4430..4990
                         /label="ORF frame 2"
         misc_feature    5000..5015
                         /label="cPPT"
         misc_feature    5000..5021
                         /label="U3PPT"
         misc_feature    5072..5105
                         /label="loxP"
         misc_feature    5125..5305
                         /label="truncHIV-1_3_LTR"
         misc_feature    5125..5305
                         /label="HIV-1_5_LTR"
         misc_feature    complement(5331..5348)
                         /label="Sp6_primer"
         gene            5586..5849
                         /label="tet (300 - 563)"
                         /gene="tet (300 - 563)"
         misc_feature    complement(5638..5657)
                         /label="pBRrevBam_primer"
         misc_feature    5990..6012
                         /label="pGEX_3_primer"
         promoter        6171..6199
                         /label="AmpR_promoter"
         gene            6241..7101
                         /label="Ampicillin"
                         /gene="Ampicillin"
         CDS             6241..7101
                         /label="ORF frame 1"
         rep_origin      7256..7875
                         /label="pBR322_origin"
         misc_feature    complement(8101..8121)
                         /label="pBABE_3_primer"
         misc_feature    complement(8107..8560)
                         /label="SV40_enhancer"
         promoter        8119..8387
                         /label="SV40_promoter"
         rep_origin      8286..8363
                         /label="SV40_origin"
         misc_feature    8348..8367
                         /label="SV40pro_F_primer"
         misc_feature    9568..9583
                         /label="SV40_int"
         misc_feature    9589..9636
                         /label="SV40_3_splice"
         CDS             9713..10186
                         /label="ORF frame 2"
         misc_feature    9764..9784
                         /label="SV40 NLS"
         misc_feature    9770..9784
                         /label="NLS"
         terminator      10212..10343
                         /label="SV40_PA_terminator"
         misc_feature    10300..10319
                         /label="EBV_rev_primer"
    ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc
     3481 tcgaggttta aactacggga tccaggccta agcttacgcg tcctagcgct accggtcgcc
     3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4261 tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa cttcgaattc
     4321 ccgggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4921 ctccccgcat cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg
     4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5041 gaagacaaga tgggatcaat tcaccatggg aataacttcg tatagcatac attatacgaa
     5101 gttatgctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5281 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
     5341 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     5401 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     5461 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     5521 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
     5581 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     5641 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     5701 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     5761 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     5821 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     5881 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     5941 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     6001 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     6061 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6121 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6181 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6241 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6301 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6361 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6421 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6481 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6541 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6601 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6661 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6721 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6781 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6841 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6901 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6961 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7021 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7081 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7141 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7201 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7261 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7321 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7381 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7441 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7501 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7561 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7621 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     7681 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7741 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7801 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7861 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7921 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7981 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8041 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     8101 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8161 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     8221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     8281 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8341 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8401 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     8461 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     8521 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     8581 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     8641 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     8701 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     8761 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     8821 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     8881 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     8941 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     9001 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     9061 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     9121 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     9181 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     9241 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     9301 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     9361 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     9421 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     9481 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     9541 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     9601 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     9661 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     9721 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     9781 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     9841 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     9901 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     9961 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
    10021 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
    10081 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
    10141 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    10201 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10321 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    10381 ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt actctaaacc
    10441 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10501 aacttagtag
    //
    

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