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  • pPRIME-TET-GFP-FF3

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Addgene
    載體名稱: pPRIME-TET-GFP-FF3
    質粒類型: 慢病毒載體;RNAi載體;四環素誘導載體
    高拷貝/低拷貝: 低拷貝
    啟動子: TET
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 8226bp
    5' 測序引物及序列: EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG
    3' 測序引物及序列: --
    載體標簽: GFP
    載體抗性: 氨芐青霉素
    篩選標記: 氯霉素
    備注: 克隆宿主菌為Stbl3,在30度條件下進行質粒擴增。
    產品目錄號: 11662
    穩定性: 穩表達
    組成型: 誘導型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pPRIME-TET-GFP-FF3載體圖譜

    載體簡介

    載體序列

    LOCUS       pPRIME-TET-GFP-FF3	8226 bp 	DNA	SYN
    DEFINITION  pPRIME-TET-GFP-FF3
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..8226
                         /organism="pPRIME-TET-GFP-FF3"
                         /mol_type="other DNA"
         promoter        239..803
                         /label="CMV_immearly_promoter"
         misc_feature    318..605
                         /label="CAG_enhancer"
         misc_feature    772..792
                         /label="CMV_fwd_primer"
         misc_feature    820..976
                         /label="HIV-1_5_LTR"
         misc_feature    820..976
                         /label="truncHIV-1_3_LTR"
         misc_feature    1087..1131
                         /label="HIV-1_psi_pack"
         CDS             1523..2410
                         /label="ORF frame 2"
    
         misc_feature    1645..1878
                         /label="RRE"
    
         misc_feature    2409..2424
                         /label="cPPT"
    
         CDS             2663..3700
                         /label="ORF frame 2"
    
         misc_feature    2882..2901
                         /label="pCEP_fwd_primer"
    
         misc_feature    2884..2908
                         /label="LNCX_primer"
    
         CDS             2928..3647
                         /label="ORF frame 3"
    
         gene            2931..3644
                         /label="EGFP"
                         /gene="EGFP"
    
         misc_feature    complement(2973..2994)
                         /label="EGFP_N_primer"
    
         misc_feature    3111..3140
                         /label="Y66 (EGFP)"
    
         misc_feature    3581..3602
                         /label="EGFP_C_primer"
    
         gene            4172..4831
                         /label="CAT/CamR"
                         /gene="CAT/CamR"
    
         CDS             4172..4831
                         /label="ORF frame 2"
    
         misc_feature    5117..5704
                         /label="WPRE"
    
         CDS             5205..5759
                         /label="ORF frame 3"
    
         CDS             5218..6258
                         /label="ORF frame 1"
    
         misc_feature    complement(5707..5723)
                         /label="pBluescriptKS_primer"
    
         misc_feature    5895..5910
                         /label="cPPT"
    
         misc_feature    5895..5916
                         /label="U3PPT"
    
         misc_feature    6232..6412
                         /label="HIV-1_5_LTR"
    
         misc_feature    6232..6412
                         /label="truncHIV-1_3_LTR"
    
         gene            complement(7233..8093)
                         /label="Ampicillin"
                         /gene="Ampicillin"
    
         CDS             complement(7233..8093)
                         /label="ORF frame 2"
    
         promoter        complement(8135..8163)
                         /label="AmpR_promoter"
    
    ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata gaccagatct gagcctggga gctctctggc
      841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      961 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     1021 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     1081 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     1141 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     1201 cagggggaaa gaaaaaatat aaataaacat atagtatggg caagcaggga gctagaacga
     1261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
     1501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     2401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2521 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
     2581 cctttcgtct tcattcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2641 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
     2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2761 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
     2821 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
     2881 cagagctcgt ttagtgaacc gtcagatcgc ctggaccggt cgccaccatg gtgagcaagg
     2941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3121 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagcgg ccgcaagcct
     3661 tgttaagtgc tcgcttcggc agcacatata ctatgtttga atgaggcttc agtactttac
     3721 agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca
     3781 acagaaggct cgagcaacca gaattcaagg ggctacttta ggagcaatta tcttgtttac
     3841 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
     3901 taaattaaat cacttttttc aattggaaga ctaatgcgtt taaacacgcg gcgacgcgtt
     3961 cgaccgaata aaacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc
     4021 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt
     4081 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     4141 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     4201 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     4261 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     4321 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     4381 tccggaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     4441 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     4501 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     4561 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     4621 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     4681 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     4741 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     4801 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     4861 aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgga
     4921 tctcgaccgc gtttgggcgg tggctccctg ccacgcggct ccgaacagaa gctgatctcc
     4981 gaagaggatc tgacatgtgt ttaaacctcg acttaattaa gtcgagggtc gacggtatcg
     5041 ataagctcgc ttcacgagat catgtttaag ggttccggtt ccactaggta caattcgata
     5101 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5521 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5701 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     5761 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5821 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5941 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     6001 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     6061 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     6121 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     6181 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
     6421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8221 cctgac
    //
    

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