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  • pLVX-TRE3G-ZsGreen1

    價格:1800元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pLVX-TRE3G-ZsGreen1, pLVX TRE3G ZsGreen1
    質粒類型: 哺乳動物細胞慢病毒表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: TRE3GV
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 9100 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐
    篩選標記: 嘌呤霉素
    備注: 包含有Tet-On 3G反式激活蛋白,多西環素(doxycycline)誘導基因表達的慢病毒載體,含有ZsGreen1綠色熒光蛋白標簽。
    產品目錄號: 631350, 631353, 631356
    穩定性: /
    組成型: 誘導型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pLVX-TRE3G-ZsGreen1載體圖譜



    pLVX-TRE3G-ZsGreen1多克隆位點

    pLVX-TRE3G-ZsGreen1載體特征

    載體簡介

    載體序列

    LOCUS       pLVX-TRE3G-ZsGreen1   9100 bp    DNA   circular             9-MAY-2012
    COMMENT     Created by Clontech Laboratories Inc.
    COMMENT     631361, 631350, 631353, 631356
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         misc_feature    1..635
                         /label=5'\LTR
         misc_feature    685..822
                         /label=packaging\signal
         misc_feature    1303..1536
                         /label=RRE
         misc_feature    2028..2151
                         /label=cPPT/CTS
         misc_feature    3881..4384
                         /label=Ppgk
         misc_feature    4405..5004
                         /label=Puro(R)
         misc_feature    5022..5613
                         /label=WPRE
         3'clip          5817..6453
                         /label=3'\LTR
         misc_feature    complement(6923..7593)
                         /label=pUC\ori
         misc_feature    3268..3856
                         /label=IRES
         misc_feature    3857..3880
                         /label=MCS
         misc_feature    2209..2560
                         /label=pTRE3GV
         misc_feature    2564..3259
                         /label=ZsGreen1
         misc_feature    complement(8599..8703)
                         /label=pAmp(R)
         misc_feature    complement(7738..8598)
                         /label=Amp(R)
    BASE COUNT     2257 a      2347 c      2391 g      2105 t 
    ORIGIN
            1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
           61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
          121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
          181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
          241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
          301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
          361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
          421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
          481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
          541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
          601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
          661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
          721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
          781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
          841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
          901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
          961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
         1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
         1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
         1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
         1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
         1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
         1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
         1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
         1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
         1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
         1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
         1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
         1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
         1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
         1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
         1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
         1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
         1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
         2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
         2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
         2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
         2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 
         2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 
         2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 
         2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 
         2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 
         2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggctc agtcaaagca 
         2581 cggcctgacc aaggagatga ccatgaagta ccgcatggag ggctgcgtgg acggccacaa 
         2641 gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcagg ccatcaacct 
         2701 gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac atcttgtccg ccgccttcat 
         2761 gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc gtcgactact tcaagaactc 
         2821 ctgccccgcc ggctacacct gggaccgctc cttcctgttc gaggacggcg ccgtgtgcat 
         2881 ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc atgtaccacg agtccaagtt 
         2941 ctacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgaccg acaactggga 
         3001 gccctcctgc gagaagatca tccccgtgcc caagcagggc atcttgaagg gcgacgtgag 
         3061 catgtacctg ctgctgaagg acggtggccg cttgcgctgc cagttcgaca ccgtgtacaa 
         3121 ggccaagtcc gtgccccgca agatgcccga ctggcacttc atccagcaca agctgacccg 
         3181 cgaggaccgc agcgacgcca agaaccagaa gtggcacctg accgagcacg ccatcgcctc 
         3241 cggatctgca ttgccctgag gccgccccgg ccggcccctc tccctccccc cccccctaac 
         3301 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc 
         3361 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg 
         3421 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg 
         3481 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc 
         3541 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa 
         3601 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa 
         3661 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta 
         3721 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg 
         3781 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 
         3841 cgatgataat atggcccccg ggacgcgtca tatggaattc taccgggtag gggaggcgct 
         3901 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 
         3961 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 
         4021 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 
         4081 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 
         4141 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 
         4201 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 
         4261 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 
         4321 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 
         4381 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc 
         4441 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 
         4501 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 
         4561 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 
         4621 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 
         4681 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 
         4741 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 
         4801 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 
         4861 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 
         4921 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 
         4981 atgacccgca agcccggtgc ctgacggggc gcgtctggaa caatcaacct ctggattaca 
         5041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
         5101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
         5161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
         5221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
         5281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
         5341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
         5401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
         5461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
         5521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
         5581 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 
         5641 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 
         5701 agaagcacaa gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac 
         5761 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg 
         5821 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac 
         5881 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga 
         5941 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata 
         6001 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga 
         6061 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc 
         6121 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg 
         6181 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct 
         6241 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc 
         6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
         6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
         6421 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 
         6481 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt 
         6541 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
         6601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
         6661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
         6721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
         6781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
         6841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
         6901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
         6961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
         7021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
         7081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
         7141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
         7201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
         7261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
         7321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
         7381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 
         7441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
         7501 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
         7561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
         7621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
         7681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
         7741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
         7801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
         7861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
         7921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
         7981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
         8041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
         8101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
         8161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
         8221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
         8281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
         8341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
         8401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
         8461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
         8521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
         8581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
         8641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
         8701 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta 
         8761 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
         8821 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
         8881 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 
         8941 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta 
         9001 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 
         9061 gaaattgtat ttgttaaata tgtactacaa acttagtagt 
    //
    

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