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  • pLVX-Puro

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pLVXpuro載體基本信息

    出品公司: Clontech
    載體名稱: pLVX-Puro , pLVXpuro
    質粒類型: 哺乳動物細胞慢病毒表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: CMV
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 8102 bp
    5' 測序引物及序列: CMV-F:CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐
    篩選標記: 嘌呤霉素
    備注: 含有組成型CMV啟動子的慢病毒載體
    產品目錄號: 632164
    穩定性: /
    組成型: --
    病毒/非病毒: 慢病毒

    pLVXpuro載體質粒圖譜和多克隆位點信息

    pLVX-Puro載體圖譜


    pLVX-Puro多克隆位點

    pLVXpuro載體簡介

    Description

    pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.

    pLVX-Puro contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-Puro also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more effi cient transduction (3). In addition to lentiviral elements, pLVX-Puro contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

    Use
    pLVX-Puro constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

    pLVXpuro載體序列

    LOCUS       pLVX-Puro	8102 bp 	DNA     circular	SYN
    DEFINITION  pLVX-Puro
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..8102
                         /organism="pLVX-Puro"
                         /mol_type="other DNA"
         misc_feature    454..634
                         /label="HIV-1_5_LTR"
         misc_feature    454..634
                         /label="truncHIV-1_3_LTR"
         misc_feature    745..789
                         /label="HIV-1_psi_pack"
         CDS             complement(1015..1476)
                         /label="ORF frame 3"
         CDS             1181..2032
                         /label="ORF frame 2"
         misc_feature    1303..1536
                         /label="RRE"
         misc_feature    2031..2046
                         /label="cPPT"
         promoter        2198..2702
                         /label="CMV_immearly_promoter"
         misc_feature    2205..2492
                         /label="CAG_enhancer"
         misc_feature    2659..2679
                         /label="CMV_fwd_primer"
         promoter        2660..2729
                         /label="CMV_promoter"
         misc_feature    2703..2722
                         /label="pCEP_fwd_primer"
         misc_feature    2705..2729
                         /label="LNCX_primer"
         misc_feature    complement(2925..2948)
                         /label="MSCV_rev_primer"
         CDS             3170..4009
                         /label="ORF frame 2"
         misc_feature    3322..3341
                         /label="mPGK_F_primer"
         gene            3410..4009
                         /label="puro (variant)"
                         /gene="puro (variant)"
         misc_feature    4025..4600
                         /label="WPRE"
         CDS             4113..4640
                         /label="ORF frame 3"
         misc_feature    5271..5451
                         /label="HIV-1_5_LTR"
         misc_feature    5271..5451
                         /label="truncHIV-1_3_LTR"
         promoter        complement(5576..5594)
                         /label="M13_reverse_primer"
         misc_feature    complement(5593..5615)
                         /label="M13_pUC_rev_primer"
         promoter        complement(5629..5658)
                         /label="lac_promoter"
         gene            complement(6741..7601)
                         /label="Ampicillin"
                         /gene="Ampicillin"
         CDS             complement(6741..7601)
                         /label="ORF frame 1"
         promoter        complement(7643..7671)
                         /label="AmpR_promoter"
         terminator      7757..7888
                         /label="SV40_PA_terminator"
         misc_feature    7845..7864
                         /label="EBV_rev_primer"
    ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagat
     2881 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     2941 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     3001 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     3061 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     3121 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     3181 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     3241 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     3301 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     3361 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
     3421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     3481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     3541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     3601 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     3661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     3721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     3781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     3841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     3901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     3961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga
     4021 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4381 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     4441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     4621 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     4681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     4741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4801 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     4861 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     4921 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     4981 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     5041 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     5101 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     5161 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     5221 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5461 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5521 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg
     5581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     7741 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7861 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     7921 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     7981 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     8041 tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     8101 gt
    //
    
    
    

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