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  • pLVX-EF1α-IRES-Puro

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pLVX-EF1α-IRES-Puro,  pLVX-EF1a-IRES-Puro,  pLVX-EF1alpha-IRES-Puro
    質粒類型: 哺乳動物細胞慢病毒表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: EF1α/ EF1a
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 8825 bp
    5' 測序引物及序列: EF-1a Forward: TCAAGCCTCAGACAGTGGTTC
    3' 測序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: 嘌呤霉素(Puromycin)
    克隆宿主菌: Stbl3
    備注: --
    產品目錄號: 631988
    穩定性: 穩表達
    組成型: 組成型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pLVX-EF1α-IRES-Puro載體圖譜



    pLVX-EF1α-IRES-Puro多克隆位點

    pLVX-EF1α-IRES-Puro載體特征

    載體簡介

    載體序列

    LOCUS       pLVX-EF1a-IRES-Puro   8825 bp    DNA   circular            12-FEB-2010
    COMMENT     Created by Clontech Laboratories Inc. 
    COMMENT     Cat. No. 631988,631253
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         LTR             1..635
                         /label=5'\LTR
         misc_feature    685..822
                         /label=Packaging\signal
         misc_feature    2028..2151
                         /label=cPPT/CTS\(DNA\flap)
         misc_feature    4794..5385
                         /label=WPRE
         rep_origin      complement(6693..7366)
                         /label=pUC\origin
         CDS             complement(7511..8507)
                         /label=Amp(R)
         misc_binding    1303..1536
                         /label=RRE
         misc_binding    636..653
                         /label=PBS
         LTR             5588..6224
                         /label=LTR
         misc_feature    3573..4148
                         /label=IRES
         CDS             4181..4780
                         /label=Puro(R)
         promoter        2185..3519
                         /label=EF1a\Promoter
         misc_feature    3535..3572
                         /label=MCS
    BASE COUNT     2140 a      2225 c      2374 g      2086 t 
    ORIGIN
            1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
           61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
          121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
          181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
          241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
          301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
          361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
          421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
          481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
          541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
          601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
          661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
          721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
          781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
          841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
          901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
          961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
         1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
         1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
         1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
         1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
         1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
         1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
         1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
         1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
         1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
         1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
         1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
         1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
         1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
         1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
         1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
         1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
         1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
         2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
         2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
         2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
         2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
         2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
         2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
         2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
         2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
         2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
         2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
         2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
         2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
         2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
         2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
         2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
         2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
         3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
         3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
         3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
         3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
         3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
         3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
         3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
         3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
         3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 
         3541 ctcgagacta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg 
         3601 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 
         3661 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 
         3721 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 
         3781 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 
         3841 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 
         3901 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 
         3961 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 
         4021 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 
         4081 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 
         4141 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac 
         4201 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt 
         4261 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac 
         4321 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 
         4381 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 
         4441 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 
         4501 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 
         4561 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 
         4621 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 
         4681 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 
         4741 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac 
         4801 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
         4861 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
         4921 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
         4981 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
         5041 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
         5101 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
         5161 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 
         5221 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
         5281 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
         5341 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag 
         5401 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga 
         5461 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt 
         5521 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
         5581 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat 
         5641 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 
         5701 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 
         5761 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 
         5821 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 
         5881 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 
         5941 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 
         6001 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 
         6061 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
         6121 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
         6181 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 
         6241 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac 
         6301 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
         6361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
         6421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
         6481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
         6541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
         6601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
         6661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
         6721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
         6781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
         6841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
         6901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
         6961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
         7021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
         7081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
         7141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
         7201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
         7261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
         7321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
         7381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
         7441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
         7501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
         7561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
         7621 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
         7681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
         7741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
         7801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
         7861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
         7921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
         7981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
         8041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
         8101 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
         8161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
         8221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
         8281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
         8341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
         8401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
         8461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 
         8521 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
         8581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
         8641 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 
         8701 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 
         8761 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 
         8821 gtagt 
    //
    

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