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  • pLVX-EF1α-AcGFP1-N1

    價格:3500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pLVX-EF1α-AcGFP1-C1, pLVX-EF1a-AcGFP1-C1, pLVX-EF1alpha-AcGFP1-C1
    質粒類型: 慢病毒表達載體
    高拷貝/低拷貝: 高拷貝
    啟動子: EF1α/ EF1a
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 9519 bp
    5' 測序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
    3' 測序引物及序列: --
    載體標簽: C-AcGFP1
    載體抗性: 氨芐青霉素
    篩選標記: 嘌呤霉素(Puromycin)
    克隆菌株: Stbl3 E.coli 
    產品目錄號: 631983
    穩定性: 穩表達
    組成型: 組成型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pLVX-EF1α-AcGFP1-N1載體圖譜



    pLVX-EF1α-AcGFP1-N1多克隆位點

    pLVX-EF1α-AcGFP1-N1載體特征

    載體簡介

    載體序列

    LOCUS       pLVX-EF1a-AcGFP1-N1   9519 bp    DNA   circular            22-MAR-2010
    COMMENT     Created by Clontech Laboratories Inc. 
    COMMENT     Cat. No. 631983
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         LTR             1..635
                         /label=5'\LTR
         misc_feature    685..822
                         /label=Packaging\signal
         misc_feature    5487..6078
                         /label=WPRE
         rep_origin      complement(7387..8060)
                         /label=pUC\origin
         CDS             complement(8205..9201)
                         /label=Amp(R)
         misc_binding    1303..1536
                         /label=RRE
         misc_binding    636..653
                         /label=PBS
         LTR             6281..6917
                         /label=3'\LTR
         misc_feature    3528..3600
                         /label=MCS
         misc_feature    3607..4326
                         /label=AcGFP1
         misc_feature    4345..4853
                         /label=PGK\Promoter
         misc_feature    4874..5473
                         /label=Puro(R)
         promoter        2186..3519
                         /label=EF1a\Promoter
         misc_feature    2028..2151
                         /label=cPPT/CTS\(DNA\flap)
    BASE COUNT     2264 a      2479 c      2598 g      2178 t 
    ORIGIN
            1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
           61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
          121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
          181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
          241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
          301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
          361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
          421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
          481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
          541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
          601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
          661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
          721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
          781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
          841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
          901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
          961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
         1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
         1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
         1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
         1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
         1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
         1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
         1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
         1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
         1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
         1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
         1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
         1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
         1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
         1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
         1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
         1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
         1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
         2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
         2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
         2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
         2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
         2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
         2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
         2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
         2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
         2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
         2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
         2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
         2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
         2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
         2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
         2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
         2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
         3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
         3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
         3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
         3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
         3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
         3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
         3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
         3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
         3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac 
         3541 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca 
         3601 ccggtcatgg tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag 
         3661 ctgaatggcg atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc 
         3721 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg 
         3781 cccaccctgg tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac 
         3841 atgaagcagc acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc 
         3901 atcttcttcg aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat 
         3961 accctggtga atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg 
         4021 ggcaataaga tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc 
         4081 aagaatggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag 
         4141 ctggccgacc actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat 
         4201 aaccactacc tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac 
         4261 atgatctact tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac 
         4321 aagtgagcgg ccgcgactct agataattct accgggtagg ggaggcgctt ttcccaaggc 
         4381 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 
         4441 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 
         4501 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 
         4561 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca 
         4621 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 
         4681 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 
         4741 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 
         4801 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca 
         4861 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 
         4921 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 
         4981 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 
         5041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 
         5101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 
         5161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 
         5221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 
         5281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 
         5341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 
         5401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 
         5461 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat 
         5521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
         5581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
         5641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
         5701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
         5761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
         5821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
         5881 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 
         5941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
         6001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
         6061 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat 
         6121 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga 
         6181 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc 
         6241 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc 
         6301 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga 
         6361 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta 
         6421 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag 
         6481 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg 
         6541 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt 
         6601 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag 
         6661 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc 
         6721 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 
         6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 
         6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 
         6901 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 
         6961 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct 
         7021 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
         7081 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
         7141 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
         7201 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
         7261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
         7321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
         7381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
         7441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
         7501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
         7561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
         7621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
         7681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
         7741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
         7801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
         7861 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
         7921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
         7981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
         8041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
         8101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
         8161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
         8221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
         8281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
         8341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
         8401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
         8461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
         8521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
         8581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
         8641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
         8701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
         8761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
         8821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
         8881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
         8941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
         9001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
         9061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
         9121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
         9181 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 
         9241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
         9301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 
         9361 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 
         9421 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 
         9481 aaattgtatt tgttaaatat gtactacaaa cttagtagt 
    //
    

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