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  • pLVX-AmCyan1-C1

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pLVX-AmCyan1-C1, pLVX AmCyan1 C1
    質粒類型: 慢病毒表達載體;熒光報告載體
    高拷貝/低拷貝: 高拷貝
    啟動子: CMV
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 8757 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: AmCyan1(N-端)
    載體抗性: 氨芐青霉素
    篩選標記: 嘌呤霉素(Puromycin)
    克隆菌株: Stbl3
    宿主細胞(系): 常規細胞系,293、CV-1、CHO等
    備注: pLVX-AmCyan1-C1載體表達N端AmCyan1融合蛋白;
    青色熒光蛋白AmCyan1具有高度可溶性,高亮度,同時與其它熒光蛋白顏色區別大。
    產品目錄號: 632557
    穩定性: 穩表達
    組成型: 組成型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pLVX-AmCyan1-C1載體圖譜



    pLVX-AmCyan1-C1多克隆位點

    pLVX-AmCyan1-C1載體特征

    載體簡介

    pLVX-AmCyan1-C1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to AmCyan1, a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (1). Genes cloned into the multiple cloning site (MCS), located at the C-terminal end of the AmCyan1 coding sequence, are expressed as C-terminal AmCyan1 fusion proteins. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the AmCyan1 coding sequence. Lentiviral particles derived from the vector allow the expression of AmCyan1 fusion proteins in virtually any cell type, including primary cells. The unmodified vector expresses AmCyan1, and may be used to produce marker virus to optimize infection protocols.
    pLVX-AmCyan1-C1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (2), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). Finally, pLVX-AmCyan1-C1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (4).In addition to lentiviral elements, pLVX-AmCyan1-C1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.
    To construct a fusion protein, the gene of interest must be cloned into pLVX-AmCyan1-C1 so that it is in-frame with the AmCyan1 coding sequence. The inserted sequence does not require an initiation codon (ATG) or a stop codon (TAA, TAG, TGA); however, if you don't want to use the stop codons downstream of the MCS (see map), you can add a stop codon to the end of your gene of interest.
    The fusion protein is constitutively expressed when pLVX-AmCyan1-C1 is transduced into target cells. Before the vector can be transduced, however, it must be transfected into 293T packaging cells with our Lenti-X HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (5).
    
    Propagation in E. coli
     Suitable host strains: DH5α, DH10B and other general purpose strains.
     Selectable marker: plasmid confers resistance to ampicillin (100 μg/ml) in E. coli hosts.
     E. coli replication origin: ColE1
     Copy number: high
    
    Excitation and emission maxima of AmCyan1
     Excitation maximum = 458 nm
     Emission maximum = 489 nm
    
    

    載體序列

    LOCUS       pLVX-AmCyan1-C1   8757 bp    DNA   circular             7-OCT-2009
    COMMENT     Created by Clontech Laboratories Inc. 
    COMMENT     Cat. No. 632557
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         LTR             1..635
                         /label=5'\LTR
         misc_feature    685..822
                         /label=packaging\signal
         misc_feature    4725..5316
                         /label=WPRE
         CDS             complement(7443..8439)
                         /label=Amp(R)
         misc_binding    1303..1536
                         /label=RRE
         misc_binding    636..653
                         /label=PBS
         LTR             5519..6155
                         /label=3'\LTR
         promoter        3583..4091
                         /label=PGK\Promoter
         CDS             4112..4711
                         /label=Puro(R)
         misc_feature    3568..3570
                         /label=Stop
         misc_feature    3572..3574
                         /label=Stop
         CDS             2807..3493
                         /label=AmCyan1
         misc_feature    2028..2151
                         /label=cPPT/CTS\(DNA\flap)
         promoter        2185..2787
                         /label=CMV\IE\Promoter
         rep_origin      complement(6625..7298)
                         /label=pUC\ori
         misc_feature    3576..3578
                         /label=Stop
         misc_feature    3507..3559
                         /label=MCS
    BASE COUNT     2166 a      2296 c      2295 g      2000 t 
    ORIGIN
            1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
           61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
          121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
          181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
          241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
          301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
          361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
          421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
          481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
          541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
          601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
          661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
          721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
          781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
          841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
          901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
          961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
         1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
         1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
         1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
         1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
         1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
         1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
         1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
         1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
         1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
         1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
         1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
         1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
         1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
         1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
         1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
         1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
         1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
         2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
         2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
         2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
         2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
         2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
         2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
         2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
         2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
         2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
         2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
         2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
         2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
         2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg ccctgtccaa 
         2821 caagttcatc ggcgacgaca tgaagatgac ctaccacatg gacggctgcg tgaacggcca 
         2881 ctacttcacc gtgaagggcg agggcagcgg caagccctac gagggcaccc agacctccac 
         2941 cttcaaggtg accatggcca acggcggccc cctggccttc tccttcgaca tcctgtccac 
         3001 cgtgttcatg tacggcaacc gctgcttcac cgcctacccc accagcatgc ccgactactt 
         3061 caagcaggcc ttccccgacg gcatgtccta cgagagaacc ttcacctacg aggacggcgg 
         3121 cgtggccacc gccagctggg agatcagcct gaagggcaac tgcttcgagc acaagtccac 
         3181 cttccacggc gtgaacttcc ccgccgacgg ccccgtgatg gccaagaaga ccaccggctg 
         3241 ggacccctcc ttcgagaaga tgaccgtgtg cgacggcatc ttgaagggcg acgtgaccgc 
         3301 cttcctgatg ctgcagggcg gcggcaacta cagatgccag ttccacacct cctacaagac 
         3361 caagaagccc gtgaccatgc cccccaacca cgtggtggag caccgcatcg ccagaaccga 
         3421 cctggacaag ggcggcaaca gcgtgcagct gaccgagcac gccgtggccc acatcacctc 
         3481 cgtggtgccc ttctccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg 
         3541 taccgcgggc ccgggatcca ccggatctag ataactgatc ataattctac cgggtagggg 
         3601 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc 
         3661 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg 
         3721 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 
         3781 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 
         3841 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 
         3901 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 
         3961 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 
         4021 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 
         4081 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct 
         4141 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 
         4201 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 
         4261 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 
         4321 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 
         4381 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 
         4441 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 
         4501 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 
         4561 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 
         4621 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 
         4681 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt 
         4741 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
         4801 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
         4861 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 
         4921 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 
         4981 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 
         5041 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
         5101 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 
         5161 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 
         5221 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 
         5281 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct 
         5341 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg 
         5401 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag 
         5461 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact 
         5521 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac 
         5581 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact 
         5641 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa 
         5701 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga 
         5761 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga 
         5821 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc 
         5881 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc 
         5941 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag 
         6001 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 
         6061 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 
         6121 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 
         6181 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc 
         6241 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 
         6301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
         6361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
         6421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
         6481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
         6541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
         6601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
         6661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
         6721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
         6781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
         6841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
         6901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
         6961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
         7021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
         7081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 
         7141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
         7201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
         7261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
         7321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
         7381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
         7441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
         7501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
         7561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
         7621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
         7681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
         7741 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
         7801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
         7861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
         7921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
         7981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
         8041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
         8101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
         8161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
         8221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
         8281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
         8341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
         8401 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt 
         8461 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
         8521 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
         8581 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata 
         8641 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga 
         8701 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt 
    //
    

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