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  • FUW-M2rtTA

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: 愛迪基因
    載體名稱: FUW-M2rtTA
    質粒類型: 慢病毒載體;四環素調控載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: Ubc
    載體大小: 7979 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: Stbl3
    宿主細胞(系): 大部分哺乳動物細胞
    備注: FUW-M2rtTA vector expressing the reverse tetracycline transactivator
    產品目錄號: #20342
    穩定性: 穩表達
    組成型/誘導型: 誘導型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    FUW-M2rtTA載體圖譜



    載體簡介

    Lentiviral plasmid expressing the reverse tetracycline transactivator.

    載體序列

    LOCUS       FUW-M2rtTA	7979 bp 	DNA	SYN
    DEFINITION  FUW-M2rtTA
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..7979
                         /organism="FUW-M2rtTA"
                         /mol_type="other DNA"
         misc_feature    7..594
                         /label="WPRE"
         CDS             95..1144
                         /label="ORF frame 2"
    
         CDS             108..620
                         /label="ORF frame 3"
    
         misc_feature    complement(597..613)
                         /label="pBluescriptKS_primer"
    
         misc_feature    781..796
                         /label="cPPT"
    
         misc_feature    781..802
                         /label="U3PPT"
    
         misc_feature    1118..1298
                         /label="HIV-1_5_LTR"
    
         misc_feature    1118..1298
                         /label="truncHIV-1_3_LTR"
    
         misc_feature    complement(1324..1341)
                         /label="BGH_rev_primer"
    
         gene            complement(2346..3206)
                         /label="Ampicillin"
                         /gene="Ampicillin"
    
         CDS             complement(2346..3206)
                         /label="ORF frame 1"
    
         promoter        complement(3248..3276)
                         /label="AmpR_promoter"
    
         promoter        3578..4154
                         /label="CMV_immearly_promoter"
    
         misc_feature    3657..3944
                         /label="CAG_enhancer"
    
         misc_feature    4111..4131
                         /label="CMV_fwd_primer"
    
         misc_feature    4174..4354
                         /label="truncHIV-1_3_LTR"
    
         misc_feature    4174..4354
                         /label="HIV-1_5_LTR"
    
         misc_feature    4465..4509
                         /label="HIV-1_psi_pack"
    
         CDS             4897..5784
                         /label="ORF frame 1"
    
         misc_feature    5019..5252
                         /label="RRE"
    
         misc_feature    5783..5798
                         /label="cPPT"
    
         promoter        5956..7172
                         /label="hUbC_promoter"
    
         CDS             7201..7947
                         /label="ORF frame 1"
    
    ORIGIN
        1 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
       61 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
      121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
      181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
      241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
      301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
      361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
      421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
      481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
      541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
      601 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
      661 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
      721 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
      781 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
      841 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
      901 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
      961 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     1021 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     1081 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     1141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     1321 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1501 gggaggattg ggaagacaat agcaggcatg tgagcaaaag gccagcaaaa ggccaggaac
     1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3181 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     3361 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     3421 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     3481 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     3541 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     3601 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     3661 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     3721 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     3781 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     3841 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     3901 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     3961 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     4021 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     4081 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     4141 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     4201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4321 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     4381 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     4441 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     4501 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     4561 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     4621 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     4681 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     4741 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     4801 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     4861 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     4921 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     4981 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     5041 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     5101 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     5161 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     5221 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     5281 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     5341 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     5401 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     5461 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     5521 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     5581 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     5641 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     5701 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     5761 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     5821 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     5881 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
     5941 cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc
     6001 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc
     6061 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
     6121 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
     6181 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
     6241 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
     6301 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
     6361 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
     6421 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
     6481 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
     6541 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     6601 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     6661 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
     6721 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
     6781 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
     6841 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
     6901 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga
     6961 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
     7021 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
     7081 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
     7141 aattctggcc gtttttggct tttttgttag acgaagcttg ggcccgggaa ttaattcacc
     7201 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc
     7261 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     7321 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     7381 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     7441 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     7501 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     7561 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     7621 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     7681 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     7741 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     7801 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     7861 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     7921 tttgaccttg acatgctccc cgggtaacta agtaaggatc aattcgatat caagcttat
    
    //
    

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