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  • pXINSECT-DEST39

    價格:3000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen
    載體名稱: pXINSECT-DEST39
    質粒類型: 昆蟲表達載體;Gateway載體
    克隆方法: Gateway
    啟動子: Actin
    載體大小: 12447 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: 6XHis tag (N-末端)
    載體抗性: 氨芐青霉素、氯霉素(僅空載體)
    真核篩選標記:
    克隆菌株: DB3.1
    宿主細胞(系): High Five(推薦) 等細胞系
    備注: pXINSECT-DEST39載體與pBmA-neo載體共轉染,可以建立穩表達細胞系;
    應用Gateway技術,可快速高效將 目的基因片段(尤其適用于目的基因片段>1kb,用傳統的連接酶法效率低的)插入到載體中;
    產品目錄號: 12249-025
    瞬表達/穩表達: 瞬表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pXINSECT-DEST39 載體圖譜



    pXINSECT-DEST39 多克隆位點

    pXINSECT-DEST39 載體特征

    載體簡介

    pXINSCET-DEST39載體優勢:
    
    Using the Express Insect Kit or the Express Insect Vector Set to achieve protein expression in insect cells has the following advantages:
     Non-lytic, continuous protein expression
     Minimal degradation of your protein of interest as compared to viral expression systems
     Higher protein expression level achieved than other similar non-viral insect expression systems
     Choice of transient or stable protein expression
     Adapted for use with the Gateway Technology or traditional restriction enzyme-mediated cloning
    
    pXINSCET-DEST39載體特征:
    
     The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
     The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
     A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
     N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
     Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
     Chloramphenicol resistance gene located between the two attR sites for counterselection
     The ccdB gene located between the two attR sites for negative selection
     The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
     Ampicillin resistance gene for selection of transformants in E. coli
     The pUC origin for high copy replication and maintenance of the plasmid in E. coli
    

    載體序列

    GAGGTCGACGGTATCGATTGCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACG
    TTGAAGTACCTTACACAACAAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCC
    AAAATCTAAAGCAAGGAGAAGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGC
    AATTTTGCAAAACTTTACTGTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAAT
    CTTCGCAGAGTCAATAAATTGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAA
    GGCACTCCCGCCGGTTTCACAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATG
    GGCCGGGCTATCAATTATCTAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAA
    CGATGTCCCCAACATACGCGGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTG
    CGGCGCATGGACCAACGTGCCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAA
    AACTTCCCGGAGACCAACGTGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTT
    ACATAATCACATGAGAACCTTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTT
    GTTTACCGCCGCCACATTAGTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTG
    CAAGGTAGAAACGCCGGAGAAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCA
    ATCTAGCTCAGTCGGATGTTAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAAC
    CAACATGTGCCAAGGATTTAACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAAC
    CCAACTTCGCCTCAATACGTAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTA
    CACGTTTAGTGATTTAATTGGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAA
    TCGTCCAACGGTAGTGACAGCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTG
    TTTTTAGGTTTAATACTTTATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTG
    GGGCACCAACTCCCATTGTTGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAA
    GACAAAAATAATATAAAATATATGTATAATTAATTAAATTCAAAAGATATGTATAATTAATTAAATTCAA
    ATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGTCGGACAACGGAACCAGACCCTG
    TAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCTCTCTCTTCGGTTCACTTTACTG
    ATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGACTAGCGTCTACATCTTTAATCT
    CGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACCGCTCTTCGTTTTTGAAAGACAA
    TACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATACTGTTCGACAATGTGCATGCTG
    CCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTCGCAATATTGTCATGATATTTAT
    AATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCGTACTTTTTTTGTTAACAATTAA
    ATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTTTACACTTTCCGAATACTTTAAT
    ATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCAATTTCATTACTCTCTTTACGAC
    TAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGTACAACTTGCTCAAAAGAAACCC
    ACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTGCGCGTAATACATATCTAAATTA
    AAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAGTGGCATTTTTTACAATTTTGTG
    CAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGCCGGTTTCTTTAACCAAATTGTA
    CGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATTATCTACCATGTAATAGTTGTTT
    GTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCAAACGGCTTTACTTCACTTTTTT
    TAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACCTATTGGAATTGTAGTCGTTGTC
    GTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGTAATTTCTTGAGTTGAAGCAACC
    GTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAGCTTTGAATTGTGCTTTTCTTGT
    ATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTGAATCGTCCAAGTATTCGTCCAG
    CTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATGCTCAAAAGATTCCGCATTATAA
    GCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCCGCATTATAAGCAGAACTAACTG
    CTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGTTATCATCAGTTAACGAATTGAC
    GCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTACCGTGTCGGCTCCATCCGGCGTG
    GGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAAAACTCTGAATAGCCGTTGTCGA
    ACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAATTAATTTGCGTCATAGTCGTTTG
    GTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAATATTCAACATGAACGTCAATTTA
    TACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCCTCTAAAACCAATGCGGTTATCG
    TTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTAATGCAAAAACTCGTTTGGTGAG
    CGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAACACCGTCCTTGAACGGAACACGA
    GGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGATTTATTTACATACAACAAGTACA
    CTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAGTGTGGCACATTTTGTCTGTGCG
    CAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGCGCACACGGACAACGGACCCGAC
    AAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTGCATTTTATTAATAACTTACAAA
    ATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCCCGAACTGCAAATCGATCGATAA
    GCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAATTAATTTCTCCGCGTCGTATTAT
    ACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATGATTTTGATGATGGAGGTGCGCT
    CAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCAATTTGCTACGACGCAATTATTG
    TGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAAAGTATATTGATATTAAATCATA
    TAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATTAAAATTGTTCAACGATTGTATG
    AGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAG
    AGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTTAAAACTAAACTCGCTTTTACGA
    GTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAACCGGCTTTAC
    GAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATTAAACTGATGTCATTTTTATACA
    CGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAACTCGCTTTACAAGTAGAATTCTA
    CTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTGTTTTCGTCATAAAATGTTTATA
    CAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATTCTACGTTACATTTAAATTCGTT
    GTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAACGTGCAACCAACGATTGTATTTG
    ACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGCACGGCTTCTTTTTTTACCACTA
    TCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACATAAGCGTACGCGTTTAAAACGTG
    CGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTTGCGTAACTCGTTTAAAGCATTA
    ATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGAACCGAGTAGTGGCATGAATAAA
    ATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCGCGGGTAGGTACCACCACCCTGC
    CTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGCGGCCGTGGTACTGAGACCTTAG
    AACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATGGGCTCCAGTAACCACTTAACAT
    CAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAAAATAAATATGTTTGAAGTCCGT
    AACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCTTCACCGACAATGGAAAATAATC
    ACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAGTTTCGATAAAGCGAGGCTACCA
    AGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACAACTTTTACCTCTCAAATTGCCC
    ACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAGCCGCTACGATATCATTATCATA
    CAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATCTCTTCATAATTATAATTAAAAA
    GAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGAGATTAATTAACCGGATTTTCTG
    GGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGAAATTCTATGAAACTTCGGCGGG
    AGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCAGTTATCAAGGCATCGCCTTATC
    AAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAAACTGAGTCAGCCCGCGATTGGT
    GGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCTTTTATATAGTTTATCCTCACGA
    GTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATCTACGTACTTGTCACTTATTTAA
    TAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACATATTTATTTATTTGTAAAATTT
    GAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTTTGAGTGTTTTTTTTTTTTAATA
    ATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGTGAAGTGAAATTTTTTATAAAAA
    AAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTACTAAATAAGAAATAAGTTTATTG
    GACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGAAATTGGGAACAGTGAATTATGA
    ATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAAATTTGTCGTTTACTTAGTATAA
    TGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCAGACTAATTCAAGAGGTGCGACG
    AAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCAGCATATG
    CGCGGTTCGCATCACCATCACCATCACAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAAC
    GAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATA
    CTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTT
    CCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
    GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCA
    TTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCG
    TAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCC
    GGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTT
    TTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTAC
    ACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAA
    TATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGAC
    AACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGG
    CGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTA
    CTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCG
    TATTTGCGCGCACCGGTGCTAGCGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTAT
    TACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGG
    TAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGG
    GATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAAT
    GCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATT
    ATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCC
    GTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGT
    GCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATT
    AACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATAGT
    GACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGA
    TATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATAATTAATTAAGATCGAAT
    CTAGAGCGGCCGCCACCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAAC
    GAAAGATTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACG
    AAACCACATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATT
    GTCCGGTGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCA
    TCGACAATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCC
    TCGCCTCCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCAT
    TGTACACAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGG
    TGGCTCAAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATA
    TGCATATTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTA
    AAGTTACTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACT
    TGCTGTCCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGA
    CAGTTACGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGA
    ATGCGAGTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTA
    CTTTCTACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTA
    GAGGTACCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACA
    CACAAGATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCA
    TTGCGTAGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTT
    AGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT
    CACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA
    CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAT
    GAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTC
    GCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA
    GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
    CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
    GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT
    CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
    ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACC
    CCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
    TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
    TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC
    AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT
    TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGG
    GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTT
    CACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT
    GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG
    CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT
    ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGC
    AGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTA
    GTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT
    TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA
    AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGG
    TTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTA
    CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGAT
    AATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT
    CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATC
    TTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG
    GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATT
    GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCC
    CCGAAAAGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAA
    TCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGAT
    AGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGG
    CGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGA
    GGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGC
    GAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTC
    ACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCAT
    TCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
    GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACG
    GCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTC
    

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