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  • pMT-Bip-V5-HisA

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen
    載體名稱: pMT-Bip-V5-HisA
    質粒類型: 昆蟲細胞表達載體
    高拷貝/低拷貝: --
    啟動子: P-MT (Metallothionein)
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 3642bp
    5' 測序引物及序列: Metallothionein: 5'd[CATCTCAGTGCAACTAAA]3'
    3' 測序引物及序列: --
    載體標簽: BiP (N), V5 (C), His (C)
    載體抗性: Ampicillin
    篩選標記: --
    備注: 使用硫酸銅或氯化鎘作為誘導劑。
    產品目錄號: V413020
    穩定性: --
    組成型: 誘導型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pMT-Bip-V5-HisA載體圖譜
    pMT-Bip-V5-HisA多克隆位點
    pMT-Bip-V5-HisA載體特征

    載體簡介

    
    

    載體序列

    LOCUS       pMT/BIP/V5-HisA           3642 bp ds-DNA    circular SYN 09-四月-2014
    DEFINITION  Drosophila vector for inducible high-level expression of secreted 
                and tagged proteins. For other reading frames, use pMT/BiP/V5-His B 
                or pMT/BiP/V5-His C.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pMT BiP V5-His A
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     To create stable cell lines, co-transfect with the drug selection 
                vector pCoHygro or pCoBlast.
    FEATURES             Location/Qualifiers
         source          1..3642
                         /organism="synthetic DNA construct"
                         /lab_host="Insect Cells"
                         /mol_type="other DNA"
         promoter        411..833
                         /label="MT promoter"
                         /label="Drosophila metallothionein promoter"
         CDS             851..904
                         /codon_start=1
                         /product="Drosophila BiP signal sequence"
                         /label="BiP signal sequence"
                         /translation="MKLCILLAVVAFVGLSLG"
         misc_feature    905..1002
                         /label="MCS"
                         /label="multiple cloning site"
         CDS             1003..1044
                         /codon_start=1
                         /product="epitope tag from simian virus 5"
                         /label="V5 tag"
                         /translation="GKPIPNPLLGLDST"
         CDS             1054..1071
                         /codon_start=1
                         /product="6xHis affinity tag"
                         /label="6xHis"
                         /translation="HHHHHH"
         polyA_signal    1144..1278
                         /label="SV40 poly(A) signal"
                         /label="SV40 polyadenylation signal"
         rep_origin      complement(1824..2412)
                         /direction=LEFT
                         /label="ori"
                         /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(2583..3443)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /label="AmpR"
                         /label="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
         promoter        complement(3444..3548)
                         /gene="bla"
                         /label="AmpR promoter"
    ORIGIN
            1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
           61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
          121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
          181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
          241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
          301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
          361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattttaa cgttgcagga
          421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
          481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
          541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata
          601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
          661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
          721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
          781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
          841 cgatctcaat atgaagttat gcatattact ggccgtcgtg gcctttgttg gcctctcgct
          901 cgggagatct ccatggcccg gggtacctac tagtccagtg tggtggaatt ctgcagatat
          961 ccagcacagt ggcggccgct cgagtctaga gggcccttcg aaggtaagcc tatccctaac
         1021 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
         1081 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
         1141 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
         1201 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
         1261 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
         1321 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
         1381 gatcagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
         1441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
         1501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
         1561 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
         1621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
         1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
         1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
         1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
         1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
         1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
         1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
         2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
         2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
         2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
         2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
         2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
         2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
         2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
         2461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
         2521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
         2581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
         2641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
         2701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
         2761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
         2821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
         2881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
         2941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
         3001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
         3061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
         3121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
         3181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
         3241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
         3301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
         3361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
         3421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
         3481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
         3541 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
         3601 acattaacct ataaaaatag gcgtatcacg aggccctttc gt
    //
    

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