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  • pIEX/Bac-1

    價格:1350元

    聯系方式:I47-825O-882O

    買家導航

    pIEXBac1載體基本信息

    出品公司: Novagen
    載體名稱: pIEXBac1, pIEXBac-1, pIEX/Bac1, pIEX/Bac-1
    質粒類型: 昆蟲細胞表達載體;蛋白純化
    啟動子: Polyhedrin
    表達水平:
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 6796 bp
    5' 測序引物: --
    3' 測序引物: --
    載體標簽: C-His, N-EK, N-Strep
    載體抗性: Ampicillin 氨芐
    備注:
    擁有hr5增強子和IE1啟動子;雙標簽系統可以采用兩種純化方案。
    產品目錄號: 71724-3
    穩定性: 瞬轉 Transient
    組成型: 組成型 Constitutive
    病毒/非病毒: 桿狀病毒

    pIEXBac1載體質粒圖譜和多克隆位點信息

    pIEX/Bac1載體圖譜


    pIEX-Bac1多克隆位點

    pIEXBac1載體簡介

    The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.

    The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.

    pIEXBac1載體序列

    LOCUS       pIEx/Bac-1   6796 bp    DNA   circular            17-OCT-2006
    DEFINITION  pIEx/BAC hr5/ie1/p10 Strep-Tag II/EkLIC/10xHis.
    SOURCE      
      ORGANISM 
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com| 
    FEATURES             Location/Qualifiers
         misc_recomb     complement(2916..4334)
                         /dnas_title="1629"
                         /vntifkey=86
                         /label=1629
         rep_origin      6271..6726
                         /dnas_title="f1 origin"
                         /vntifkey=33
                         /label=f1 origin
         CDS             complement(5282..6139)
                         /dnas_title="Ap"
                         /vntifkey=4
                         /label=Ap
         rep_origin      complement(4521..4521)
                         /dnas_title="origin"
                         /vntifkey=33
                         /label=origin
         misc_recomb     4..453
                         /dnas_title="lef-2"
                         /vntifkey=86
                         /label=lef-2
         misc_recomb     complement(491..1096)
                         /dnas_title="ORF 603"
                         /vntifkey=86
                         /label=ORF 603
         rep_origin      4354..5113
                         /dnas_title="pUC Ori"
                         /vntifkey=33
                         /label=pUC Ori
         enhancer        1196..1677
                         /dnas_title="hr5"
                         /vntifkey=9
                         /label=hr5
         polyA_signal    2742..2747
                         /dnas_title="PolyA signal"
                         /vntifkey=25
                         /label=PolyA signal
         5'UTR           2229..2278
                         /dnas_title="ie1 5'-UTR"
                         /vntifkey=52
                         /label=ie1 5'-UTR
         promoter        2279..2391
                         /dnas_title="p10 promoter + 5'UTR"
                         /vntifkey=29
                         /label=p10 promoter + 5'UTR
         promoter        1682..2228
                         /dnas_title="ie1 promoter"
                         /vntifkey=29
                         /label=ie1 promoter
         terminator      2565..2871
                         /dnas_title="ie1 Terminator"
                         /vntifkey=43
                         /label=ie1 Terminator
         protein_bind    2433..2447
                         /dnas_title="Ek site"
                         /vntifkey=31
                         /label=Ek site
         protein_bind    2403..2426
                         /dnas_title="Strep-Tag II"
                         /vntifkey=31
                         /label=Strep-Tag II
         misc_binding    2517..2546
                         /dnas_title="10x His•Tag"
                         /vntifkey=20
                         /label=10x His•Tag
    BASE COUNT     1824 a   1462 c   1506 g   2004 t
    ORIGIN      
              1 GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG
             61 AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC
            121 AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT
            181 GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG
            241 GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC
            301 CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT
            361 AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC
            421 TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT
            481 TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA
            541 TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC
            601 AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT
            661 CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT
            721 ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT
            781 TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT
            841 TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT
            901 TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA
            961 TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT
           1021 TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT
           1081 TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA
           1141 TCAATATATA GTTGCTGATC AGATCTGATC ATGGAGATAA TTAAAATGAT AAGGGCGCGT
           1201 AAAACACAAT CAAGTATGAG TCATAAGCTG ATGTCATGTT TTGCACACGG CTCATAACCG
           1261 AACTGGCTTT ACGAGTAGAA TTCTACTTGT AACGCACGAT CAGTGGATGA TGTCATTTGT
           1321 TTTTCAAATC GAGATGATGT CATGTTTTGC ACACGGCTCA TAAACTCGCT TTACGAGTAG
           1381 AATTCTACGT GTAACGCACG ATCGATTGAT GAGTCATTTG TTTTGCAATA TGATATCATA
           1441 CAATATGACT CATTTGTTTT TCAAAACCGA ACTTGATTTA CGGGTAGAAT TCTACTTGTA
           1501 AAGCACAATC AAAAAGATGA TGTCATTTGT TTTTCAAAAC TGAACTCGCT TTACGAGTAG
           1561 AATTCTACGT GTAAAACACA ATCAAGAAAT GATGTCATTT GTTATAAAAA TAAAAGCTGA
           1621 TGTCATGTTT TGCACATGGC TCATAACTAA ACTCGCTTTA CGGGTAGAAT TCTACGCGCG
           1681 TCGATGTCTT TGTGATGCGC GCGACATTTT TGTAGGTTAT TGATAAAATG AACGGATACG
           1741 TTGCCCGACA TTATCATTAA ATCCTTGGCG TAGAATTTGT CGGGTCCATT GTCCGTGTGC
           1801 GCTAGCATGC CCGTAACGGA CCTCGTACTT TTGGCTTCAA AGGTTTTGCG CACAGACAAA
           1861 ATGTGCCACA CTTGCAGCTC TGCATGTGTG CGCGTTACCA CAAATCCCAA CGGCGCAGTG
           1921 TACTTGTTGT ATGCAAATAA ATCTCGATAA AGGCGCGGCG CGCGAATGCA GCTGATCACG
           1981 TACGTCCCTC GTGTTCCGTT CAAGGACGGT GTTATCGACC TCAGATTAAT GTTTATCGGC
           2041 CGACTGTTTT CGTATCCGCT CACCAAACGC GTTTTTGCAT TAACATTGTA TGTCGGCGGA
           2101 TGTTCTATAT CTAATTTGAA TAAATAAACG ATAACCGCGT TGGTTTTAGA GGGCATAATA
           2161 AAAGAAATAT TGTTATCGTG TTCGCCATTA GGGCAGTATA AATTGACGTT CATGTTGGAT
           2221 ATTGTTTCAG TTGCAAGTTG ACACTGGCGG CGACAAGATC GTGAACAACC AAGTGACTCC
           2281 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
           2341 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCCATGGCAA
           2401 GCTGGAGCCA CCCGCAGTTC GAAAAGGGTG CAGATGACGA CGACAAGGTA CCGGATCCGA
           2461 CGTCGGTCGA CAAGCTTGCG GCCGCAGGAG CTCCGGGCTT CTCCTCAATT TCCGCTCATC
           2521 ACCACCATCA TCACCATCAC CACCACTAAG TGATTAACCT CAGGTTATAC ATATATTTTG
           2581 AATTTAATTA ATTATACATA TATTTTATAT TATTTTTGTC TTTTATTATC GAGGGGCCGT
           2641 TGTTGGTGTG GGGTTTTGCA TAGAAATAAC AATGGGAGTT GGCGACGTTG CTGCGCCAAC
           2701 ACCACCTCCC TTCCCTCCTT TCATCATGTA TCTGTAGATA AAATAAAATA TTAAACCTAA
           2761 AAACAAGACC GCGCCTATCA ACAAAATGAT AGGCATTAAC TTGCCGCTGA CGCTGTCACT
           2821 AACGTTGGAC GATTTGCCGA CTAAACCTTC ATCGCCCAGT AACCAATCTA GGTAGCTGAG
           2881 CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT
           2941 TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG
           3001 TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT
           3061 AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC
           3121 GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT
           3181 TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG
           3241 ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT
           3301 TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT
           3361 TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC
           3421 GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA
           3481 TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT
           3541 CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT
           3601 AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC
           3661 ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT
           3721 GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT
           3781 GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG
           3841 TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC
           3901 GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC
           3961 GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT
           4021 GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG
           4081 GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA
           4141 TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA
           4201 TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG
           4261 ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC
           4321 TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
           4381 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
           4441 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
           4501 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
           4561 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
           4621 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
           4681 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
           4741 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
           4801 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
           4861 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
           4921 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
           4981 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
           5041 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
           5101 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
           5161 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
           5221 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
           5281 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
           5341 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
           5401 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
           5461 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
           5521 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
           5581 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
           5641 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
           5701 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
           5761 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
           5821 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
           5881 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
           5941 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
           6001 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
           6061 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
           6121 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
           6181 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
           6241 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
           6301 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
           6361 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
           6421 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
           6481 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
           6541 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
           6601 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
           6661 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA
           6721 CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
           6781 CTTCGCTATT ACGCCA
    //
    
    

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