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  • pFastBacHT A

    價格:850元

    聯系方式:I47-825O-882O

    買家導航

    pFastBacHTA載體基本信息

    出品公司: Invitrogen
    載體名稱: pFastBacHTA, pFastBac HTA, pFastBacHT A, pFastBac HT A
    質粒類型: 昆蟲細胞表達載體;桿狀病毒表達載體
    啟動子: Polyhedrin
    表達水平:
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 4856 bp
    5' 測序引物: pFastbac-F: TATTCCGGATTATTCATACC
    3' 測序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
    載體標簽: N-His tag
    載體抗性: Ampicillin 氨芐
    篩選標記: Gentamicin 慶大霉素
    備注:
    含TEV切割位點
    產品目錄號: 10584-027
    穩定性: 穩表達 Stable
    組成型: 組成型 Constitutive
    病毒/非病毒: 桿狀病毒

    pFastBacHTA載體質粒圖譜和多克隆位點信息

    pFastBacHT A 載體圖譜



    pFastBacHT A 多克隆位點

    pFastBacHT A 載體特征

    pFastBacHTA載體簡介

    Bac-to-BacHT Vector Kit 設計用于在大腸桿菌 (E. coli) 中生成桿狀病毒載體后,在 Sf9、Sf21 或 High Five 細胞中表達和純化帶組氨酸標簽的重組蛋白。 pFastBacHT 載體具
    
    有以下特點:
    
     用于表達蛋白質的多角體強啟動子
    
     用于簡化克隆的三個讀碼框
    
     用于輕松純化重組融合蛋白的 N-端 6xHis 標簽
    
     用于在蛋白質純化后去除組氨酸標簽的 TEV 蛋白酶切割位點
    
    
    
    引用及參考文獻
    
    DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
    Hey F, Czyzewicz N, Jones P, Sablitzky F, 
    J Biol Chem (2012) 287:31073-31084
    Product usage: na
    
    Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
    Dan C; Kelly A; Bernard O; Minden A; 
    J Biol Chem (2001) 276:32115-32136
    Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.
    
    Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
    Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M; 
    J Biol Chem (2002) 277:9780-9789
    Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu, 
    
    and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.
    

    pFastBacHTA載體序列

    LOCUS       pFastBacTHT\A           4856 bp    DNA     circular SYN  2-SEP-2003
    SOURCE      
      ORGANISM  
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..4856
                         /invitrogen="605"
                         /vntifkey="98"
         CDS             589..1449
                         /invitrogen="1020000"
                         /vntifkey="4"
                         /label=Amp(R)
         promoter        490..588
                         /invitrogen="2330000"
                         /vntifkey="30"
                         /label=bla\promoter
         rep_origin      2..457
                         /invitrogen="2520000"
                         /vntifkey="33"
                         /label=f1\origin
         CDS             complement(2802..3335)
                         /invitrogen="1150000"
                         /vntifkey="4"
                         /label=Gm(R)
         misc_feature    4119..4222
                         /invitrogen="1520000"
                         /vntifkey="21"
                         /label=MCS
         promoter        complement(3524..3551)
                         /invitrogen="2250000"
                         /vntifkey="29"
                         /label=Pc\promoter
         promoter        3904..4032
                         /invitrogen="2260000"
                         /vntifkey="29"
                         /label=PH\promoter
         primer          3920..3937
                         /invitrogen="2060000"
                         /vntifkey="27"
                         /label=Polyhedrin\forward\primer
         rep_origin      1594..2267
                         /invitrogen="2540000"
                         /vntifkey="33"
                         /label=pUC\origin
         polyA_signal    4240..4480
                         /invitrogen="1850000"
                         /vntifkey="25"
                         /label=SV40\pA
         CDS             4101..4121
                         /invitrogen="1300000"
                         /vntifkey="4"
                         /label=TEV\recognition\site
         misc_recomb     4499..4693
                         /invitrogen="2750000"
                         /vntifkey="86"
                         /label=Tn7L
         misc_recomb     2512..2735
                         /invitrogen="2760000"
                         /vntifkey="86"
                         /label=Tn7R
         CDS             4062..4079
                         /invitrogen="1015000"
                         /vntifkey="4"
                         /label=6xHis
         CDS             4050..4052
                         /invitrogen="123000000"
                         /vntifkey="4"
                         /label=initiation\ATG
    BASE COUNT     1219 a      1197 c      1152 g      1288 t
    ORIGIN
            1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
           61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
          121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
          181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
          241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
          301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
          361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
          421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
          481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
          541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
          601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
          661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
          721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
          781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
          841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
          901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
          961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
         1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
         1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
         1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
         1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
         1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
         1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
         1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
         1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
         1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
         1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
         1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
         1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
         1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
         1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
         1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
         1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
         1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
         2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
         2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
         2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
         2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
         2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
         2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
         2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
         2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
         2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
         2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
         2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
         2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
         2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
         2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
         2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
         2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
         3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
         3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
         3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
         3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
         3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
         3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
         3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
         3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
         3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
         3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
         3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
         3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
         3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
         3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
         3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
         3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
         3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
         4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
         4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggat ccggaattca
         4141 aaggcctacg tcgacgagct caactagtgc ggccgctttc gaatctagag cctgcagtct
         4201 cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca gccataccac
         4261 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
         4321 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
         4381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
         4441 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
         4501 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
         4561 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
         4621 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
         4681 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
         4741 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
         4801 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgg
    //

    pFastBacHTA載體質粒圖譜pdf版和相關資料下載

    pFastBacHTA載體應用舉例

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