• <sub id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track>
  • <option id="w5dj7"><li id="w5dj7"><option id="w5dj7"></option></li></option>
  • <xmp id="w5dj7"><center id="w5dj7"></center></xmp>
  • <sub id="w5dj7"></sub>
  • <sub id="w5dj7"></sub>
  • <progress id="w5dj7"><center id="w5dj7"><tbody id="w5dj7"></tbody></center></progress> <sub id="w5dj7"></sub>
  • <track id="w5dj7"><li id="w5dj7"></li></track>
  • <track id="w5dj7"><noframes id="w5dj7"><track id="w5dj7"></track>
  • <nav id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track><track id="w5dj7"></track>
  • pCoHygro

    價格:1250元

    聯系方式:I47-825O-882O

    買家導航

    pCoHygro載體基本信息

    出品公司: Invitrogen
    載體名稱: pCoHygro
    質粒類型: 昆蟲表達載體;篩選載體
    克隆方法: --
    啟動子: --
    載體大小: 4526 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽:
    載體抗性: 氨芐青霉素
    真核篩選標記: hygromycin
    克隆菌株: TOP10;DH5-T1R
    宿主細胞(系): 果蠅細胞系S2
    備注: pCoHygro載體昆蟲載體共轉染,建立穩表達細胞系。
    產品目錄號: K4130-01
    瞬表達/穩表達: --
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    pCoHygro載體質粒圖譜和多克隆位點信息

    pCoHygro載體圖譜
    pCoHygro 載體特征

    pCoHygro載體簡介

    pCoHygro載體序列

    LOCUS       pCoHygro           4526 bp ds-DNA    circular SYN 07-April-2014
    DEFINITION  Selection plasmid for insect cells, with a hygromycin resistance 
                gene under control of the copia promoter.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pCoHygro
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..4526
                         /organism="synthetic DNA construct"
                         /lab_host="Insect Cells"
                         /mol_type="other DNA"
         promoter        501..780
                         /label="copia promoter"
                         /label="strong promoter from the Drosophila transposable 
                         element copia?(Sinclair et al., 1986)"
         CDS             805..1830
                         /codon_start=1
                         /product="hygromycin B phosphotransferase"
                         /label="HygR"
                         /label="confers resistance to hygromycin"
         polyA_signal    2143..2277
                         /label="SV40 poly(A) signal"
                         /label="SV40 polyadenylation signal"
         rep_origin      complement(2707..3295)
                         /direction=LEFT
                         /label="ori"
                         /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(3466..4326)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /label="AmpR"
                         /label="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
         promoter        complement(4327..4431)
                         /gene="bla"
                         /label="AmpR promoter"
    ORIGIN
            1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
           61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
          121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
          181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
          241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
          301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
          361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
          421 actctagagg atccggtgcg tggtggtttc atgcttctgg gaacggcaaa tgggtttagg
          481 attgggaacc cctcatcatc tgttggaata tactattcaa cctacaaaaa taacgttaaa
          541 caacactact ttatatttga tatgaatggc cacacctttt atgccataaa acatattgta
          601 agagaatacc actcttttta ttccttcttt ccttcttgta cgttttttgc tgtgagtagg
          661 tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata aatcataaaa ctcaaacata
          721 aacttgacta tttatttatt tattaagaaa ggaaatataa attataaatt acaacaggtt
          781 atggggatcc ccagcttggc caccatggga aagcctgagc gtaccgcgac gtctgtcgag
          841 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
          901 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
          961 tgcggcgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
         1021 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
         1081 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
         1141 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
         1201 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
         1261 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
         1321 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
         1381 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
         1441 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
         1501 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
         1561 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
         1621 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
         1681 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
         1741 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
         1801 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt
         1861 cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc
         1921 ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg
         1981 gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg
         2041 tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc
         2101 ctgccgattg ggtgcagatc agcctcgagg ccagctagct tgaacttgtt tattgcagct
         2161 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
         2221 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg
         2281 taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
         2341 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
         2401 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
         2461 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
         2521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
         2581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
         2641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
         2701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
         2761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
         2821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
         2881 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
         2941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
         3001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
         3061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
         3121 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
         3181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
         3241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
         3301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
         3361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
         3421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
         3481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
         3541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
         3601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
         3661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
         3721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
         3781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
         3841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
         3901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
         3961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
         4021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
         4081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
         4141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
         4201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
         4261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
         4321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
         4381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
         4441 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
         4501 taggcgtatc acgaggccct ttcgtc
    //
    

    pCoHygro載體質粒圖譜pdf版和相關資料下載

    pCoHygro載體應用舉例

    分享到:

    全部載體分類

    相關載體

    相關文章

    相關問答

    Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng 滬ICP備2021037978號-1
    歡迎您的訪問和咨詢....
    富二代app最新安卓下载
  • <sub id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track>
  • <option id="w5dj7"><li id="w5dj7"><option id="w5dj7"></option></li></option>
  • <xmp id="w5dj7"><center id="w5dj7"></center></xmp>
  • <sub id="w5dj7"></sub>
  • <sub id="w5dj7"></sub>
  • <progress id="w5dj7"><center id="w5dj7"><tbody id="w5dj7"></tbody></center></progress> <sub id="w5dj7"></sub>
  • <track id="w5dj7"><li id="w5dj7"></li></track>
  • <track id="w5dj7"><noframes id="w5dj7"><track id="w5dj7"></track>
  • <nav id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track><track id="w5dj7"></track>