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  • pAcGHLT C

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pAcGHLTC載體基本信息

    出品公司: BD Biosciences
    載體名稱: pAcGHLT C
    質粒類型: 昆蟲細胞載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: --
    啟動子: --
    載體大小: 8753 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: --
    宿主細胞(系): --
    備注: --
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    pAcGHLTC載體質粒圖譜和多克隆位點信息

    pAcGHLT C載體圖譜

    pAcGHLTC載體簡介

    pAcGHLTC載體序列

    LOCUS       pAcGHLT C                8753 bp ds-DNA     circular SYN 19-APR-2014
    DEFINITION  Baculovirus transfer vector for expressing proteins with an 
                N-terminal GST-6xHis-PKA-thrombin cassette. For other reading 
                frames, use pAcGHLT A or pAcGHLT B.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pAcGHLT C
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 8753)
      AUTHORS   BD Biosciences
      TITLE     Direct Submission
      JOURNAL   Exported Thursday, February 6, 2020 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..8753
                         /organism="synthetic DNA construct"
                         /lab_host="Insect Cells"
                         /mol_type="other DNA"
         misc_recomb     1269..2096
                         /note="baculovirus recombination region (lef2/ORF603)"
                         /note="contains ORF603 and part of lef2"
         CDS             1269..1393
                         /codon_start=3
                         /product="baculovirus late expression factor 2"
                         /note="lef2"
                         /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
         CDS             complement(1431..2036)
                         /codon_start=1
                         /product="baculovirus ORF603 protein"
                         /note="ORF603"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                         IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                         KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                         INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
         promoter        2100..2191
                         /gene="polh from Autographa californica"
                         /note="polyhedrin promoter"
                         /note="promoter for the baculovirus polyhedrin gene"
         CDS             2206..2859
                         /codon_start=1
                         /product="glutathione S-transferase from Schistosoma 
                         japonicum"
                         /note="GST"
                         /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                         FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                         GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                         YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
         CDS             2875..2892
                         /codon_start=1
                         /product="6xHis affinity tag"
                         /note="6xHis"
                         /translation="HHHHHH"
         CDS             2899..2916
                         /codon_start=1
                         /product="recognition site for phosphorylation by protein 
                         kinase A"
                         /note="PKA site"
                         /translation="RRASVA"
         CDS             2926..2943
                         /codon_start=1
                         /product="thrombin recognition and cleavage site"
                         /note="thrombin site"
                         /translation="LVPRGS"
         misc_feature    2958..3025
                         /note="MCS"
                         /note="multiple cloning site"
         misc_recomb     3045..4503
                         /note="baculovirus recombination region (ORF1629)"
                         /note="contains part of ORF1629"
         CDS             complement(3162..4503)
                         /codon_start=2
                         /product="baculovirus capsid-associated protein"
                         /note="ORF1629"
                         /note="required for viral replication"
                         /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                         LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                         PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                         PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                         APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                         EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                         PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                         IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
         rep_origin      5876..6331
                         /direction=RIGHT
                         /note="f1 ori"
                         /note="f1 bacteriophage origin of replication; arrow 
                         indicates direction of (+) strand synthesis"
         promoter        6613..6717
                         /gene="bla"
                         /note="AmpR promoter"
         CDS             6718..7578
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         rep_origin      7749..8337
                         /direction=RIGHT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
    ORIGIN
            1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
           61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
          121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
          181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
          241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
          301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
          361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
          421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
          481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
          541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
          601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
          661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
          721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
          781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
          841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
          901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
          961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
         1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
         1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
         1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
         1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
         1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
         1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
         1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
         1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
         1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
         1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
         1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
         1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
         1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
         1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
         1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
         1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
         1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
         2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
         2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
         2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
         2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
         2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
         2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
         2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
         2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
         2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
         2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
         2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
         2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
         2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
         2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatccgat gggacatcat
         2881 catcatcatc acggaaggag aagggccagt gttgcggcgg gaattttggt ccctcgtgga
         2941 agcccaggac tcgatggcat atgctcgagg aattcaggcc tccatgggag ctcgcggccg
         3001 cctgcagggt acccccggga gatctgtacc gactctgctg aagaggagga aattctcctt
         3061 gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct gttcactggt
         3121 ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct agattctgtg
         3181 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
         3241 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
         3301 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
         3361 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
         3421 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
         3481 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
         3541 tacaattgac tcgacgtaaa cacgttaaat aaagcttgga catatttaac atcgggcgtg
         3601 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
         3661 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
         3721 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
         3781 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
         3841 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
         3901 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
         3961 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
         4021 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
         4081 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
         4141 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
         4201 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
         4261 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
         4321 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
         4381 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
         4441 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
         4501 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
         4561 agacacttcg ctgtcgtcga cgtacatgta tgctttgttg tcaaaaacgt cgttggcaag
         4621 ctttaaaata tttaaaagaa catctctgtt cagcaccact gtgttgtcgt aaatgttgtt
         4681 tttgataatt tgcgcttccg cagtatcgac acgttcaaaa aattgatgcg catcaatttt
         4741 gttgttccta ttattgaata aataagattg tacagattca tatctacgat tcgtcatggc
         4801 caccacaaat gctacgctgc aaacgctggt acaattttac gaaaactgca aaaacgtcaa
         4861 aactcggtat aaaataatca acgggcgctt tggcaaaata tctattttat cgcacaagcc
         4921 cactagcaaa ttgtatttgc agaaaacaat ttcggcgcac aattttaacg ctgacgaaat
         4981 aaaagttcac cagttaatga gcgaccaccc aaattttata aaaatctatt ttaatcacgg
         5041 ttccatcaac aaccaagtga tcgtgatgga ctacattgac tgtcccgatt tatttgaaac
         5101 actacaaatt aaaggcgagc tttcgtacca acttgttagc aatattatta gacagctgtg
         5161 tgaagcgctc aacgatttgc acaagcacaa tttcatacac aacgacataa aactcgaaaa
         5221 tgtcttatat ttcgaagcac ttgatcgcgt gtatgtttgc gattacggat tgtgcaaaca
         5281 cgaaaactca cttagcgtgc acgacggcac gttggagtat tttagtccgg aaaaaattcg
         5341 acacacaact atgcacgttt cgtttgactg gtacgcggcg tgttaacata caagttgcta
         5401 accggcggcc gacacccatt tgaaaaaagc gaagacgaaa tgttggactt gaatagcatg
         5461 aagcgtcgtc agcaatacaa tgacattggc gttttaaaac acgttcgtaa cgttaacgct
         5521 cgtgactttg tgtactgcct aacaagatac aacatagatt gtagactcac aaattacaaa
         5581 caaattataa aacatgagtt tttgtcgtaa aaatgccact tgttttacga gtagaattag
         5641 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
         5701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
         5761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
         5821 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
         5881 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
         5941 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
         6001 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
         6061 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
         6121 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
         6181 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
         6241 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
         6301 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
         6361 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
         6421 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
         6481 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
         6541 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
         6601 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
         6661 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
         6721 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
         6781 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
         6841 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
         6901 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
         6961 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
         7021 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
         7081 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
         7141 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
         7201 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
         7261 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
         7321 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
         7381 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
         7441 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
         7501 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
         7561 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
         7621 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
         7681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
         7741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
         7801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
         7861 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
         7921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
         7981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
         8041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
         8101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
         8161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
         8221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
         8281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
         8341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
         8401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
         8461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
         8521 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
         8581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
         8641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
         8701 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga att
    //
    
    
    

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