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  • pAcG2T

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: BD Biosciences
    載體名稱: pAcG2T
    質粒類型: 昆蟲細胞載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: --
    啟動子: --
    載體大小: 8530 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: --
    宿主細胞(系): --
    備注: --
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    載體質粒圖譜和多克隆位點信息

    pAcG2T載體圖譜

    載體簡介

    載體序列

    LOCUS       pAcG2T                8530 bp ds-DNA     circular SYN 19-APR-2014
    DEFINITION  Baculovirus transfer vector for expressing N-terminally GST-tagged 
                proteins with a thrombin cleavage site, for use with the BD 
                BaculoGold(TM) system.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pAcG2T
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 8530)
      AUTHORS   BD Biosciences
      TITLE     Direct Submission
      JOURNAL   Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..8530
                         /organism="synthetic DNA construct"
                         /lab_host="Insect Cells"
                         /mol_type="other DNA"
         misc_recomb     1269..2096
                         /note="baculovirus recombination region (lef2/ORF603)"
                         /note="contains ORF603 and part of lef2"
         CDS             1269..1393
                         /codon_start=3
                         /product="baculovirus late expression factor 2"
                         /note="lef2"
                         /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
         CDS             complement(1431..2036)
                         /codon_start=1
                         /product="baculovirus ORF603 protein"
                         /note="ORF603"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                         IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                         KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                         INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
         promoter        2100..2191
                         /gene="polh from Autographa californica"
                         /note="polyhedrin promoter"
                         /note="promoter for the baculovirus polyhedrin gene"
         CDS             2206..2859
                         /codon_start=1
                         /product="glutathione S-transferase from Schistosoma 
                         japonicum"
                         /note="GST"
                         /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                         FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                         GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                         YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
         CDS             2866..2883
                         /codon_start=1
                         /product="thrombin recognition and cleavage site"
                         /note="thrombin site"
                         /translation="LVPRGS"
         misc_feature    2878..2893
                         /note="MCS"
                         /note="multiple cloning site"
         misc_recomb     2923..4280
                         /note="baculovirus recombination region (ORF1629)"
                         /note="contains part of ORF1629"
         CDS             complement(2939..4280)
                         /codon_start=2
                         /product="baculovirus capsid-associated protein"
                         /note="ORF1629"
                         /note="required for viral replication"
                         /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                         LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                         PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                         PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                         APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                         EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                         PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                         IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
         rep_origin      5653..6108
                         /direction=RIGHT
                         /note="f1 ori"
                         /note="f1 bacteriophage origin of replication; arrow 
                         indicates direction of (+) strand synthesis"
         promoter        6390..6494
                         /gene="bla"
                         /note="AmpR promoter"
         CDS             6495..7355
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         rep_origin      7526..8114
                         /direction=RIGHT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
    ORIGIN
            1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
           61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
          121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
          181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
          241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
          301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
          361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
          421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
          481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
          541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
          601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
          661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
          721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
          781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
          841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
          901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
          961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
         1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
         1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
         1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
         1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
         1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
         1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
         1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
         1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
         1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
         1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
         1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
         1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
         1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
         1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
         1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
         1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
         1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
         2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
         2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
         2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
         2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
         2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
         2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
         2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
         2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
         2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
         2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
         2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
         2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
         2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
         2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctggt tccgcgtgga
         2881 tccccgggaa ttcatcgtga ctgactgacg atctgagatc cggttattag tacatttatt
         2941 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
         3001 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
         3061 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
         3121 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
         3181 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
         3241 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
         3301 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
         3361 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
         3421 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
         3481 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
         3541 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
         3601 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
         3661 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
         3721 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
         3781 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
         3841 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
         3901 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
         3961 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
         4021 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
         4081 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
         4141 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
         4201 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
         4261 gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact
         4321 acagcattgt agtggcgaga cacttcgctg tcgtcgacgt acatgtatgc tttgttgtca
         4381 aaaacgtcgt tggcaagctt taaaatattt aaaagaacat ctctgttcag caccactgtg
         4441 ttgtcgtaaa tgttgttttt gataatttgc gcttccgcag tatcgacacg ttcaaaaaat
         4501 tgatgcgcat caattttgtt gttcctatta ttgaataaat aagattgtac agattcatat
         4561 ctacgattcg tcatggccac cacaaatgct acgctgcaaa cgctggtaca attttacgaa
         4621 aactgcaaaa acgtcaaaac tcggtataaa ataatcaacg ggcgctttgg caaaatatct
         4681 attttatcgc acaagcccac tagcaaattg tatttgcaga aaacaatttc ggcgcacaat
         4741 tttaacgctg acgaaataaa agttcaccag ttaatgagcg accacccaaa ttttataaaa
         4801 atctatttta atcacggttc catcaacaac caagtgatcg tgatggacta cattgactgt
         4861 cccgatttat ttgaaacact acaaattaaa ggcgagcttt cgtaccaact tgttagcaat
         4921 attattagac agctgtgtga agcgctcaac gatttgcaca agcacaattt catacacaac
         4981 gacataaaac tcgaaaatgt cttatatttc gaagcacttg atcgcgtgta tgtttgcgat
         5041 tacggattgt gcaaacacga aaactcactt agcgtgcacg acggcacgtt ggagtatttt
         5101 agtccggaaa aaattcgaca cacaactatg cacgtttcgt ttgactggta cgcggcgtgt
         5161 taacatacaa gttgctaacc ggcggccgac acccatttga aaaaagcgaa gacgaaatgt
         5221 tggacttgaa tagcatgaag cgtcgtcagc aatacaatga cattggcgtt ttaaaacacg
         5281 ttcgtaacgt taacgctcgt gactttgtgt actgcctaac aagatacaac atagattgta
         5341 gactcacaaa ttacaaacaa attataaaac atgagttttt gtcgtaaaaa tgccacttgt
         5401 tttacgagta gaattagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
         5461 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
         5521 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
         5581 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
         5641 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
         5701 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
         5761 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
         5821 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
         5881 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
         5941 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
         6001 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
         6061 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
         6121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
         6181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
         6241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
         6301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
         6361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
         6421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
         6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
         6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
         6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
         6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
         6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
         6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
         6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
         6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
         6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
         7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
         7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
         7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
         7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
         7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
         7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
         7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
         7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
         7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
         7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
         7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
         7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
         7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
         7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
         7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
         7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
         7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
         8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
         8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
         8161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
         8221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
         8281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
         8341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
         8401 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
         8461 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
         8521 attacgaatt
    //
    
    
    

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