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  • pYM39-EYFP

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pYM39EYFP載體基本信息

    出品公司: --
    載體名稱: pYM39-EYFP
    質粒類型: 酵母系列,畢赤酵母表達載體
    終止子: ADH1
    復制子: ori
    啟動子: TEF
    載體大小: 4946bp
    5' 測序引物及序列: T7:TAATACGACTCACTATAGGG
    3' 測序引物及序列: --
    載體標簽: EYFP
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: DH5α 等
    宿主細胞(系): 酵母細胞
    備注: --
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    pYM39EYFP載體質粒圖譜和多克隆位點信息

    pYM39-EYFP載體圖譜

    pYM39EYFP載體簡介

    pYM39EYFP載體序列

    LOCUS       pYM39-EYFP               4946 bp ds-DNA     circular SYN 19-SEP-2016
    DEFINITION  synthetic circular DNA
    ACCESSION   .
    VERSION     .
    KEYWORDS    Untitled 10
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 4946)
      AUTHORS   .
      TITLE     Direct Submission
      JOURNAL   Exported Monday, September 19, 2016 from SnapGene Viewer 3.2.1
                http://www.native-nursery.com/
    FEATURES             Location/Qualifiers
         source          1..4946
                         /organism="synthetic DNA construct"
                         /mol_type="other DNA"
         CDS             79..795
                         /codon_start=1
                         /product="enhanced YFP"
                         /note="EYFP"
                         /note="mammalian codon-optimized"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                         FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                         NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                         NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                         FVTAAGITLGMDELYK"
         CDS             802..828
                         /codon_start=1
                         /product="HA (human influenza hemagglutinin) epitope tag"
                         /note="HA"
                         /translation="YPYDVPDYA"
         terminator      861..1048
                         /gene="S. cerevisiae ADH1"
                         /note="ADH1 terminator"
                         /note="transcription terminator for the S. cerevisiae 
                         alcohol dehydrogenase 1 (ADH1) gene"
         gene            1123..2479
                         /note="kanMX"
                         /note="yeast selectable marker conferring kanamycin 
                         resistance (Wach et al., 1994)"
         promoter        1123..1466
                         /note="TEF promoter"
                         /note="Ashbya gossypii TEF promoter"
         CDS             1467..2276
                         /codon_start=1
                         /gene="aph(3')-Ia"
                         /product="aminoglycoside phosphotransferase"
                         /note="KanR"
                         /note="confers resistance to kanamycin"
                         /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                         DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                         FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                         FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                         RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
         terminator      2282..2479
                         /note="TEF terminator"
                         /note="Ashbya gossypii TEF terminator"
         promoter        complement(2584..2602)
                         /note="T7 promoter"
                         /note="promoter for bacteriophage T7 RNA polymerase"
         rep_origin      complement(2860..3448)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(3619..4479)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(4480..4584)
                         /gene="bla"
                         /note="AmpR promoter"
         promoter        4930..2
                         /note="SP6 promoter"
                         /note="promoter for bacteriophage SP6 RNA polymerase"
    ORIGIN
            1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
           61 ggtgctggtg ctggagcaat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
          121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
          181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
          241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tgcagtgctt cgcccgctac
          301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
          361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
          421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
          481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
          541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
          601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
          661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
          721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
          781 gagctgtaca agtaaggatc ctatccatat gacgttccag attacgctgc tcagtgctag
          841 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
          901 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
          961 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
         1021 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga
         1081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
         1141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
         1201 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
         1261 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc
         1321 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
         1381 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
         1441 cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc
         1501 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
         1561 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
         1621 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
         1681 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
         1741 catggttact caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc
         1801 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
         1861 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
         1921 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
         1981 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
         2041 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
         2101 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
         2161 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
         2221 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
         2281 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
         2341 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
         2401 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
         2461 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tttaaacgag
         2521 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt
         2581 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg
         2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
         2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
         2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
         2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
         2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
         2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
         3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
         3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
         3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
         3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
         3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
         3301 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
         3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
         3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
         3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
         3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
         3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
         3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
         3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
         3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
         3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
         3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
         3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
         4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
         4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
         4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
         4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
         4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
         4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
         4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
         4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
         4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
         4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
         4621 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
         4681 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
         4741 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
         4801 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
         4861 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
         4921 acacatacga tttaggtgac actata
    //
    
    
    

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