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  • pYC52

    價格:1200元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Irene Castano Navarro(2015)
    載體名稱: pYC52
    質粒類型: 釀酒酵母表達載體;整合型載體;FLP/FRT系統載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: --
    載體大小: 6639 bp
    5' 測序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
    3' 測序引物及序列: GFP-R primer 5’ ACCATCTAATTCAACAAGAATTGGGACAAC 3’
    載體標簽: 青色熒光蛋白CFP (C ter)
    載體抗性: 氨芐青霉素
    篩選標記: URA3,Nourseothricin
    克隆菌株: DH10B感受態細胞 ,30°C
    宿主細胞(系): 出芽酵母(釀酒酵母)、白色念珠菌
    備注: --
    產品目錄號: #63908
    穩定性: 瞬表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pYC52載體圖譜



    pYC52多克隆位點

    載體簡介

    釀酒酵母整合型載體還有: 

    pYC44
    pYC56   紅色熒光標簽
    pYC54   黃色熒光標簽
    pYC52   青色熒光標簽
    pYC50   13XMyc標簽
    pYC48   HA標簽
    pYC46   3XFlag標簽



    載體序列

    LOCUS       pYC52	6639 bp 	DNA	SYN
    DEFINITION  pYC52
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..6639
                         /organism="pYC52"
                         /mol_type="other DNA"
         misc_feature    complement(29..51)
                         /label="pGEX_3_primer"
         promoter        196..409
                         /label="URA3_promoter"
         gene            411..1211
                         /label="URA3"
                         /gene="URA3"
         CDS             411..1214
                         /label="ORF frame 3"
                         /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                         LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                         LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                         TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                         TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
         CDS             complement(446..922)
                         /label="ORF frame 3"
                         /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                         GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                         EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
         misc_feature    complement(1814..1962)
                         /label="lacZ_a"
                         /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                         GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                         EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
         misc_feature    1928..1950
                         /label="M13_pUC_fwd_primer"
                         /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                         GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                         EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
         promoter        1943..1959
                         /label="M13_forward20_primer"
                         /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                         GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                         EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
         promoter        1966..1984
                         /label="T7_promoter"
                         /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                         GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                         EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
         misc_feature    2017..2033
                         /label="pBluescriptSK_primer"
                         /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                         GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                         EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
         CDS             2037..2753
                         /label="ORF frame 3"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                         IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITLGMDELYK*"
         gene            2043..2750
                         /label="GFP (variant)"
                         /gene="GFP (variant)"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                         IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITLGMDELYK*"
         misc_feature    complement(2067..2095)
                         /label="GFP-R primer"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                         IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITLGMDELYK*"
         misc_feature    complement(2111..2130)
                         /label="GFPfusionREV_primer"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                         IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITLGMDELYK*"
         misc_feature    2217..2246
                         /label="Y66W (ECFP)"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                         IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITLGMDELYK*"
         misc_feature    2690..2710
                         /label="GFP_F_primer"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                         IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITLGMDELYK*"
         CDS             complement(3445..3948)
                         /label="ORF frame 1"
                         /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
                         NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
                         INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
                         PVVYVRM*"
         CDS             3484..4056
                         /label="ORF frame 1"
                         /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                         FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                         NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                         GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
         promoter        complement(4378..4397)
                         /label="T3_promoter"
                         /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                         FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                         NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                         GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
         promoter        complement(4414..4432)
                         /label="M13_reverse_primer"
                         /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                         FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                         NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                         GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
         misc_feature    complement(4431..4453)
                         /label="M13_pUC_rev_primer"
                         /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                         FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                         NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                         GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
         rep_origin      complement(4805..5424)
                         /label="pBR322_origin"
                         /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                         FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                         NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                         GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
         gene            complement(5579..6439)
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                         FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                         NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                         GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
         CDS             complement(5579..6439)
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        complement(6481..6509)
                         /label="AmpR_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
      421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
      481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
      541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
      601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
      661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
      721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
      781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
      841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
      901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
      961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
     1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
     1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
     1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
     1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
     1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
     1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
     1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
     1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
     1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
     1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
     1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
     1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
     1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctaatgA
     2041 GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG
     2101 TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC
     2161 TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGCCA ACACTTGTCA
     2221 CTACTTTGAC TTGGGGTGTT CAATGCTTTG CTAGATACCC AGATCATATG AAACAGCATG
     2281 ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA TTTTTCAAAG
     2341 ATGACGGGAA CTACAAGACA CGTGCTGAAG TCAAGTTTGA AGGTGATACC CTTGTTAATA
     2401 GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA CACAAATTGG
     2461 AATACAACGC TATTTCAGAT AATGTATACA TCACTGCAGA CAAACAAAAG AATGGAATCA
     2521 AAGCTAATTT CAAAATTAGA CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT
     2581 ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT
     2641 CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG
     2701 AGTTTGTAAC AGCTGCTGGG ATTACACTAG GCATGGATGA ACTATACAAA tagagatccg
     2761 aagttcctat actttctaga gaataggaac ttcgtgcgct tttgaaccac gtaaagtgct
     2821 gtaaaacagc tctattcaat gaggtaaatg ataaagttta taagagacca aaaaattcaa
     2881 aaaaaaaatt gaaaaattat attggtattt atatgaaaca atagttcagt tcaaagcgct
     2941 agcttttctc atctcaacta accggtatta ggtgggtatt tatttaatat gggaacaaga
     3001 aatgtgacac taatgttcat gtttttattt tcattattta ttctatatgg tttgggactt
     3061 gatgttatgt cttaattcat agaGATCCgc ttgcctcgtc cccgccgggt cacccggcca
     3121 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
     3181 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
     3241 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg
     3301 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
     3361 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
     3421 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccgGAT Cgatcctaca
     3481 attATGTCTA CTACTTTGGA TGATACTGCT TATAGATACA GAACTTCTGT TCCAGGTGAT
     3541 GCTGAAGCTA TTGAAGCTTT GGATGGTTCT TTCACTACCG ATACTGTTTT TAGAGTTACT
     3601 GCTACTGGTG ATGGTTTCAC TTTGAGAGAA GTTCCAGTTG ATCCACCATT GACTAAGGTT
     3661 TTCCCAGATG ATGAATCCGA TGATGAATCC GATGCTGGTG AAGATGGTGA TCCAGATTCT
     3721 AGAACTTTCG TTGCTTATGG TGATGATGGT GATTTGGCTG GTTTCGTTGT TGTTTCTTAT
     3781 TCTGGTTGGA ACAGAAGATT GACTGTTGAA GATATTGAAG TTGCTCCAGA ACATAGAGGT
     3841 CATGGTGTTG GTAGAGCTTT GATGGGTTTG GCTACTGAAT TCGCCAGAGA AAGAGGTGCT
     3901 GGTCATTTGT GGTTGGAAGT TACCAATGTT AATGCTCCAG CTATTCATGC TTATAGAAGA
     3961 ATGGGTTTCA CTTTGTGTGG TTTGGATACT GCTTTATACG ATGGTACTGC TTCCGATGGT
     4021 GAACAAGCTT TGTATATGTC TATGCCATGT CCATAAtaac TCGACcagta ctgacaataa
     4081 aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct
     4141 attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga
     4201 tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc
     4261 tatactgctg tcgattcgat actaacgccg ccatccagtg TCGAtcgacG AAGTTCCTAT
     4321 ACTTTCTAGA GAATAGGAAC TTCGTCctcg agggggggcc cggtaccagc ttttgttccc
     4381 tttagtgagg gttaattccg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4441 attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct
     4501 ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc
     4561 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4621 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4681 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4741 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4801 aggcCGCGTT GCTGGCGTTT TTCCATAGGC TCGGCCCCCC TGACGAGCAT CACAAAAATC
     4861 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTCCCCC
     4921 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     4981 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT
     5041 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     5101 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     5161 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     5221 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
     5281 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     5341 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     5401 GATCTCAAGA AGATCCTTTG ATCTtttcta cggggtctga cgctcagtgg aacgaaaact
     5461 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5521 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagTT
     5581 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     5641 TTGCCTGACT GCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     5701 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     5761 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     5821 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     5881 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     5941 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGA AAAAAAGCGG
     6001 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     6061 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     6121 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     6181 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     6241 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     6301 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     6361 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     6421 GGAAATGTTG AATACTCATa ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6541 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6601 taacctataa aaataggcgt atcacgaggc cctttcgtc
    //
    
    

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