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  • pAbAi

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pAbAi
    質粒類型: 酵母單雜交系統
    高拷貝/低拷貝: 高拷貝
    克隆方法: --
    啟動子: --
    載體大小: 4894 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: URA3
    克隆菌株: --
    宿主細胞(系): --
    備注: --
    產品目錄號: --
    穩定性: --
    組成型/誘導型: --
    病毒/非病毒: --

    載體質粒圖譜和多克隆位點信息

    pAbAi載體圖譜

    載體簡介

    載體序列

    LOCUS       pAbAi                4894 bp ds-DNA     circular SYN 19-OCT-2017
    DEFINITION  Yeast integrating vector for cloning a DNA bait sequence to be used 
                in a one-hybrid screen.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pAbAi
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 4894)
      AUTHORS   Clontech (TaKaRa)
      TITLE     Direct Submission
      JOURNAL   Exported Saturday, January 11, 2020 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    COMMENT     Linearize with BstBI or BbsI to integrate at the URA3 locus.
    FEATURES             Location/Qualifiers
         source          1..4894
                         /organism="synthetic DNA construct"
                         /lab_host="Saccharomyces cerevisiae"
                         /mol_type="other DNA"
         misc_feature    1..45
                         /note="MCS"
                         /note="multiple cloning site"
         CDS             185..1390
                         /codon_start=1
                         /gene="S. cerevisiae AUR1 with a dominant F158Y mutation"
                         /product="aureobasidin A-resistant mutant of inositol 
                         phosphorylceramide synthase"
                         /note="AurR"
                         /note="confers fungal resistance to Aureobasidin A"
                         /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW
                         VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT
                         SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV
                         VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP
                         GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV
                         CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS
                         KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK
                         RA"
         rep_origin      complement(2047..2635)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(2806..3666)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(3667..3771)
                         /gene="bla"
                         /note="AmpR promoter"
         CDS             complement(3875..4678)
                         /codon_start=1
                         /gene="S. cerevisiae URA3"
                         /product="orotidine-5'-phosphate decarboxylase, required 
                         for uracil biosynthesis"
                         /note="URA3"
                         /note="yeast auxotrophic marker, counterselectable with 
                         5-fluoroorotic acid (5-FOA)"
                         /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                         ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                         YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                         YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                         VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
         promoter        complement(4679..4894)
                         /gene="S. cerevisiae URA3"
                         /note="URA3 promoter"
    ORIGIN
            1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat
           61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc
          121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt
          181 aataatggca aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt
          241 agccgattta gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa
          301 acccgcttta agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt
          361 cattactaat cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt
          421 attcatcatt ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt
          481 ggcgctgtat ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa
          541 agtgttacca gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac
          601 atcgacgaat tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg
          661 ggccccattt gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg
          721 ttatgctttt gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc
          781 agccgctccc ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca
          841 tggctcgcct ggtggattag ctagaattga taagctactc ggtattaata tgtatactac
          901 atgtttttca aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc
          961 tactatggaa gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc
         1021 ttatgtttgc tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct
         1081 tatggcaggt tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc
         1141 aattgtagat acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat
         1201 atcaaagagt gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa
         1261 cttggaactt gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt
         1321 tgatggatct acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa
         1381 gagggcttaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
         1441 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
         1501 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
         1561 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac
         1621 tctagcgaat taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
         1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
         1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
         1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
         1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
         1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
         1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
         2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
         2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
         2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
         2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
         2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
         2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
         2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
         2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
         2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
         2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
         2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
         2701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
         2761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
         2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
         2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
         2941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
         3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
         3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
         3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
         3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
         3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
         3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
         3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
         3421 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
         3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
         3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
         3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
         3661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
         3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
         3781 ccgaaaagtg ccacctgggt aataactgat ataattaaat tgaagctcta atttgtgagt
         3841 ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca
         3901 aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct
         3961 ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca
         4021 cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca
         4081 taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga
         4141 taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag
         4201 atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta
         4261 cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat
         4321 tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg
         4381 tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata
         4441 atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg
         4501 tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg
         4561 tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct
         4621 tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga
         4681 tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat
         4741 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc
         4801 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc
         4861 aaaaaaaaga ataaaaaaaa aatgatgaat tgaa
    //
    
    
    

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