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  • pET-3a(+)

    價格:800元

    聯系方式:I47-825O-882O

    買家導航

    pet3a載體基本信息

    出品公司: EMD Biosciences(Novagen)
    別名: pET3a, pet 3a
    質粒類型: 大腸桿菌蛋白表達
    表達水平:
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 4640bp
    5' 測序引物: T7
    5' 測序引物序列:   5'-TAATACGACTCACTATAGGG-3'
    載體標簽: N-T7
    載體抗性: Ampicillin
    備注:
    Same as pET9 but ampR; a,b,c,d vary by MCS.
    產品目錄號:  69418-3, 69419-3, 69420-3, 69421-3
    穩定性: 瞬時表達 Transient
    組成型: 組成型 Constitutive
    病毒/非病毒: 非病毒
    表達宿主菌: BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)
    pET表達手冊: pET表達手冊英文版
    pET表達手冊中文版
    pET載體匯總:
    pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
    pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
    pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
    pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
    pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
    pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
    pET-51b   pET-52b  
    pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

    pet3a載體質粒圖譜和多克隆位點信息

    pet3a載體圖譜
    pET-3a多克隆位點

    pet3a載體簡介

    pet3a載體序列

    LOCUS       pET-3a(+)    4640 bp     DNA    SYN
    DEFINITION  pET-3a
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     ORIGDB|GenBank
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTOAUTHORAD1|Novagen Inc.|
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..4640
                         /organism="pET-3a"
                         /mol_type="other DNA"
         gene            86..1553
                         /label="tet (w/ gaps)"
                         /gene="tet (w/ gaps)"
         terminator      complement(379..507)
                         /label="T7_terminator"
         misc_feature    447..465
                         /label="T7_Terminal_primer"
         misc_feature    complement(519..551)
                         /label="T7_leader"
         misc_feature    complement(534..552)
                         /label="Xpress_fwd_primer"
         misc_feature    complement(560..576)
                         /label="T7_transl_en_RBS"
         promoter        complement(613..631)
                         /label="T7_promoter"
         misc_feature    complement(715..734)
                         /label="pBRrevBam_primer"
         CDS             768..1553
                         /label="ORF frame 3"
         CDS             complement(900..1358)
                         /label="ORF frame 1"
         misc_feature    2192..2383
                         /label="ROP"
         misc_feature    complement(2377..2399)
                         /label="pGEX_3_primer"
         gene            complement(3572..4432)
                         /label="Ampicillin"
                         /gene="Ampicillin"
         CDS             complement(3572..4432)
                         /label="ORF frame 2"
         promoter        complement(4474..4502)
                         /label="AmpR_promoter"
    ORIGIN
        1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
       61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
      121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
      181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA
      241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG
      301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC
      361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA
      421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT
      481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCGCGAC CCATTTGCTG TCCACCAGTC
      541 ATGCTAGCCA TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGAAAC
      601 CGTTGTGGTC TCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC TCGATCCTCT
      661 ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG TGCGGTTGCT GGCGCCTATA
      721 TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG AGCGCTTGTT
      781 TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC ATCTCCTTGC
      841 ATGCACCATT CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC
      901 TAATGCAGGA GTCGCATAAG GGAGAGCGTC GACCGATGCC CTTGAGAGCC TTCAACCCAG
      961 TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT
     1021 TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTG GGTCATTTTC GGCGAGGACC
     1081 GCTTTCGCTG GAGCGCGACG ATGATCGGCC TGTCGCTTGC GGTATTCGGA ATCTTGCACG
     1141 CCCTCGCTCA AGCCTTCGTC ACTGGTCCCG CCACCAAACG TTTCGGCGAG AAGCAGGCCA
     1201 TTATCGCCGG CATGGCGGCC GACGCGCTGG GCTACGTCTT GCTGGCGTTC GCGACGCGAG
     1261 GCTGGATGGC CTTCCCCATT ATGATTCTTC TCGCTTCCGG CGGCATCGGG ATGCCCGCGT
     1321 TGCAGGCCAT GCTGTCCAGG CAGGTAGATG ACGACCATCA GGGACAGCTT CAAGGATCGC
     1381 TCGCGGCTCT TACCAGCCTA ACTTCGATCA CTGGACCGCT GATCGTCACG GCGATTTATG
     1441 CCGCCTCGGC GAGCACATGG AACGGGTTGG CATGGATTGT AGGCGCCGCC CTATACCTTG
     1501 TCTGCCTCCC CGCGTTGCGT CGCGGTGCAT GGAGCCGGGC CACCTCGACC TGAATGGAAG
     1561 CCGGCGGCAC CTCGCTAACG GATTCACCAC TCCAAGAATT GGAGCCAATC AATTCTTGCG
     1621 GAGAACTGTG AATGCGCAAA CCAACCCTTG GCAGAACATA TCCATCGCGT CCGCCATCTC
     1681 CAGCAGCCGC ACGCGGCGCA TCTCGGGCAG CGTTGGGTCC TGGCCACGGG TGCGCATGAT
     1741 CGTGCTCCTG TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT TGCCTTACTG GTTAGCAGAA
     1801 TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC TGCAAAACGT CTGCGACCTG
     1861 AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA AGTCTGGAAA CGCGGAAGTC
     1921 AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC TACCCTGTGG
     1981 AACACCTACA TCTGTATTAA CGAAGCGCTG GCATTGACCC TGAGTGATTT TTCTCTGGTC
     2041 CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT TCCAGTAACC GGGCATGTTC
     2101 ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC ATCGGTATCA TTACCCCCAT
     2161 GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA
     2221 CATGGCCCGC TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA
     2281 CGCGGATGAA CAGGCAGACA TCTGTGAATC GCTTCACGAC CACGCTGATG AGCTTTACCG
     2341 CAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA
     2401 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC
     2461 AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT
     2521 GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATATGC
     2581 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT
     2641 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     2701 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     2761 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     2821 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     2881 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     2941 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     3001 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     3061 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     3121 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     3181 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     3241 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     3301 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     3361 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     3421 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     3481 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     3541 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     3601 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     3661 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
     3721 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     3781 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     3841 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTGCA GGCATCGTGG
     3901 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     3961 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     4021 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     4081 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     4141 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA
     4201 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     4261 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     4321 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     4381 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     4441 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     4501 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
     4561 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
     4621 GGCCCTTTCG TCTTCAAGAA
    //
    

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