• <sub id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track>
  • <option id="w5dj7"><li id="w5dj7"><option id="w5dj7"></option></li></option>
  • <xmp id="w5dj7"><center id="w5dj7"></center></xmp>
  • <sub id="w5dj7"></sub>
  • <sub id="w5dj7"></sub>
  • <progress id="w5dj7"><center id="w5dj7"><tbody id="w5dj7"></tbody></center></progress> <sub id="w5dj7"></sub>
  • <track id="w5dj7"><li id="w5dj7"></li></track>
  • <track id="w5dj7"><noframes id="w5dj7"><track id="w5dj7"></track>
  • <nav id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track><track id="w5dj7"></track>
  • pET-21c(+)

    價格:800元

    聯系方式:I47-825O-882O

    買家導航

    pET21c載體基本信息

    出品公司: EMD Biosciences(Novagen)
    別名: pET21c, pet 21c
    質粒類型: 大腸桿菌蛋白表達
    表達水平:
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 5441bp
    5' 測序引物: T7
    5' 測序引物序列:   5'-TAATACGACTCACTATAGGG-3'
    載體標簽: N-T7, C-His
    載體抗性: Ampicillin
    備注:
    Same as pET24a, b, c, d(+) but ampR; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
    產品目錄號:  69742-3
    穩定性: 瞬時表達 Transient
    組成型: 組成型 Constitutive
    病毒/非病毒: 非病毒
    表達宿主菌: BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)
    pET表達手冊: pET表達手冊英文版
    pET表達手冊中文版
    pET載體匯總:
    pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
    pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
    pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
    pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
    pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
    pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
    pET-51b   pET-52b  
    pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

    pET21c載體質粒圖譜和多克隆位點信息

    pET-21c載體圖譜



    pET-21c多克隆位點

    pET-21c載體特征

    Feature Name Start End  
    T7_terminator 129 1
    T7_Terminal_primer 69 87
    T7_leader 237 205
    Xpress_fwd_primer 238 220
    T7_transl_en_RBS 262 246
    lacO 307 280
    T7_promoter 325 307
    tet (300 - 563) 357 620
    pBRrevBam_primer 428 409
    lacI 703 1794
    tet (576 - 851) 1853 2128
    ROP 2603 2794
    pGEX_3_primer 2810 2788
    pBR322_origin 3828 3209
    Ampicillin 4843 3983
    AmpR_promoter 4913 4885
    ORF Start End  
    ORF frame 3 462 962
    ORF frame 1 835 1794
    ORF frame 2 4843 3983
    Enzyme Name Cut
    XhoI 158
    NotI 166
    EagI 166
    HindIII 173
    SalI 179
    SacI 190
    EcoRI 192
    BamHI 198
    NheI 229
    NdeI 236
    XbaI 274
    BglII 340
    BclI 1076
    ApaI 1273
    EcoRV 1512
    HpaI 1568
    PstI 4301

    pET21c載體簡介

    pET-21a-d載體具有N端T7標簽和C端His標簽。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是,這些載體序列是依據載體pBR322的方向來進行編號的,所以T7標簽表達區域在載體圖譜上是反向的。

    pET Vectors 選擇指南

    pET21c載體序列

    LOCUS       pET-21c (+)    5441 bp     DNA  Circular  SYN
    DEFINITION  pET-21 c (+)
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     ORIGDB|GenBank|
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTOAUTHORAD1|Novagen Inc.|
    FEATURES             Location/Qualifiers
         source          1..5441
                         /organism="pET-21 c (+)"
                         /mol_type="other DNA"
         terminator      complement(1..129)
                         /label="T7_terminator"
         misc_feature    69..87
                         /label="T7_Terminal_primer"
         misc_feature    complement(205..237)
                         /label="T7_leader"
         misc_feature    complement(220..238)
                         /label="Xpress_fwd_primer"
         misc_feature    complement(246..262)
                         /label="T7_transl_en_RBS"
         misc_feature    complement(280..307)
                         /label="lacO"
         promoter        complement(307..325)
                         /label="T7_promoter"
         gene            357..620
                         /label="tet (300 - 563)"
                         /gene="tet (300 - 563)"
         misc_feature    complement(409..428)
                         /label="pBRrevBam_primer"
         CDS             462..962
                         /label="ORF frame 3"
                         /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                         LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                         RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                         ANCRGD*"
         misc_feature    703..1794
                         /label="lacI"
                         /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                         LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                         RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                         ANCRGD*"
         CDS             835..1794
                         /label="ORF frame 1"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         gene            1853..2128
                         /label="tet (576 - 851)"
                         /gene="tet (576 - 851)"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         misc_feature    2603..2794
                         /label="ROP"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         misc_feature    complement(2788..2810)
                         /label="pGEX_3_primer"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         rep_origin      complement(3209..3828)
                         /label="pBR322_origin"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         gene            complement(3983..4843)
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         CDS             complement(3983..4843)
                         /label="ORF frame 2"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        complement(4885..4913)
                         /label="AmpR_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
      241 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      301 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      601 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
      661 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
      721 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
      781 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
      841 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
      901 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
      961 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     1021 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     1081 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     1141 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     1201 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     1261 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     1321 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     1381 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     1441 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     1501 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     1561 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     1621 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     1681 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1801 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
     1861 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     1921 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     1981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     2041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     2101 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct
     2161 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     2221 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     2281 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
     2341 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
     2401 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     2461 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
     2521 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
     2581 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     2641 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     2701 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     2761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
     3001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     3061 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3121 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3901 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3961 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4021 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4081 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4141 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4201 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4261 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     4321 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4381 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4441 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4501 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4561 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4621 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4681 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4741 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4801 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4861 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4921 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg
     4981 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     5041 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     5101 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     5161 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     5221 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     5281 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     5341 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     5401 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a
    //
    

    pET21c載體質粒圖譜pdf版和相關資料下載

    pET21c載體應用舉例

    分享到:

    全部載體分類

    相關載體

    相關文章

    相關問答

    Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng 滬ICP備2021037978號-1
    歡迎您的訪問和咨詢....
    富二代app最新安卓下载
  • <sub id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track>
  • <option id="w5dj7"><li id="w5dj7"><option id="w5dj7"></option></li></option>
  • <xmp id="w5dj7"><center id="w5dj7"></center></xmp>
  • <sub id="w5dj7"></sub>
  • <sub id="w5dj7"></sub>
  • <progress id="w5dj7"><center id="w5dj7"><tbody id="w5dj7"></tbody></center></progress> <sub id="w5dj7"></sub>
  • <track id="w5dj7"><li id="w5dj7"></li></track>
  • <track id="w5dj7"><noframes id="w5dj7"><track id="w5dj7"></track>
  • <nav id="w5dj7"><xmp id="w5dj7"><track id="w5dj7"></track><track id="w5dj7"></track>