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  • pTrcHis2 B

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    pTrcHis2B載體基本信息

    出品公司: Invitrogen
    別名: pTrcHis2B、 pTrcHis2 B
    質粒類型: 大腸桿菌表達載體
    表達水平: 高拷貝
    啟動子: Trc
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 4404 bp
    5' 測序引物及序列: pTrcHis-F: gaggtatatattaatgtatcg
    3' 測序引物及序列: pTrcHis-R:GATTTAATCTGTATCAGG
    載體標簽: C-His, C-Myc
    載體抗性: Ampicillin
    備注:
    Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420.
    產品目錄號: V365-20
    穩定性: 瞬時表達 Transient
    組成型: 組成型 Constitutive
    病毒/非病毒: 非病毒

    pTrcHis2B載體質粒圖譜和多克隆位點信息

    pTrcHis2B載體圖譜



    pTrcHis2B多克隆位點

    pTrcHis2B載體特征

    pTrcHis2B載體簡介

    The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.

    Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.

    pTrcHis2B載體序列

    LOCUS       pTrcHis2\B              4404 bp    DNA     circular SYN  7-JUL-2013
    DEFINITION  pTrcHis2 B.
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    COMMENT     http://www.native-nursery.com/
    COMMENT     This file is created by Vector NTI
                http://www.invitrogen.com/
    COMMENT     ORIGDB|GenBank
    COMMENT     VNTNAME|pTrcHis2 B|
    COMMENT     VNTOAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..4404
                         /organism="pTrcHis2 A"
                         /mol_type="other DNA"
                         /vntifkey="98"
         misc_feature    235..257
                         /vntifkey="21"
                         /label=M13_pUC_rev_primer
         misc_feature    264..333
                         /vntifkey="21"
                         /label=rrnB_ATS
         misc_feature    370..390
                         /vntifkey="21"
                         /label=pTrcHis_fwd_primer
         misc_feature    383..409
                         /vntifkey="21"
                         /label=minicis
         misc_feature    complement(587..604)
                         /vntifkey="21"
                         /label=pBAD_rev_primer
         misc_feature    complement(587..604)
                         /vntifkey="21"
                         /label=pTrcHis_rev_primer
         terminator      637..794
                         /vntifkey="43"
                         /label=rrnB_terminator
         terminator      760..803
                         /vntifkey="43"
                         /label=rrnB_T1_terminator
         terminator      935..962
                         /vntifkey="43"
                         /label=rrnB_T2_terminator
         promoter        1004..1032
                         /vntifkey="30"
                         /label=AmpR_promoter
         gene            1074..1934
                         /gene="Ampicillin"
                         /vntifkey="60"
         CDS             1074..1934
                         /vntifkey="4"
                         /label=ORF\frame\2
         rep_origin      2089..2708
                         /vntifkey="33"
                         /label=pBR322_origin
         misc_feature    3105..3127
                         /vntifkey="21"
                         /label=pGEX_3_primer
         misc_feature    3274..4365
                         /vntifkey="21"
                         /label=lacI
         CDS             3406..4365
                         /vntifkey="4"
                         /label=ORF\frame\3
    BASE COUNT     1091 a      1098 c      1160 g      1055 t
    ORIGIN
            1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
           61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
          121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
          181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
          241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
          301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
          361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatggatcc
          421 gagctcgaga tctgcagctg gtaccatatg ggaattcgaa gctttctaga acaaaaactc
          481 atctcagaag aggatctgaa tagcgccgtc gaccatcatc atcatcatca ttgagtttaa
          541 acggtctcca gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
          601 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
          661 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
          721 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
          781 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
          841 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
          901 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
          961 tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc
         1021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
         1081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
         1141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
         1201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
         1261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
         1321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
         1381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
         1441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
         1501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
         1561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
         1621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
         1681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
         1741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
         1801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
         1861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
         1921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
         1981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
         2041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
         2101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
         2161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
         2221 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
         2281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
         2341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
         2401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
         2461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
         2521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
         2581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
         2641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
         2701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
         2761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
         2821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
         2881 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
         2941 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
         3001 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
         3061 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
         3121 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa
         3181 ggcgaagcgg catgcattta cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg
         3241 gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta
         3301 tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag
         3361 gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat
         3421 tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt
         3481 gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
         3541 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
         3601 tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
         3661 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
         3721 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
         3781 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
         3841 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
         3901 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
         3961 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
         4021 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
         4081 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtca
         4141 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
         4201 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
         4261 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
         4321 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
         4381 tgtgagttag cgcgaattga tctg
    //

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