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  • pSP72

    價格:750元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Promega
    載體名稱: pSP72
    質粒類型: 克隆載體;雙向啟動子載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: T7 和 SP6
    載體大小: 2462 bp
    5' 測序引物及序列: SP6 fwd:ATTTAGGTGACACTATAG
    3' 測序引物及序列: T7 fwd:TAATACGACTCACTATAGGG
    載體標簽:
    載體抗性: 氨芐青霉素
    宿主菌株: DH5alpha
    備注: 含有SP6和T7兩個RNA聚合酶識別位點,可進行體外轉錄。兩個啟動子方向相反。
    產品目錄號: P2191
    穩定性: Unknown
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pSP72載體圖譜



    pSP72 多克隆位點

    pSP72 載體特征

    載體簡介

    載體序列

    LOCUS       X65332                  2462 bp    DNA     circular SYN 25-JAN-2000
    DEFINITION  Cloning vector pSP72.
    ACCESSION   X65332
    VERSION     X65332.2  GI:6759494
    KEYWORDS    beta-lactamase; bla gene; cloning vector; multiple cloning site;
                promoter.
    SOURCE      Cloning vector pSP72
      ORGANISM  Cloning vector pSP72
                other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..2462
                         /organism="Cloning vector pSP72"
                         /mol_type="other DNA"
                         /db_xref="taxon:90137"
         regulatory      join(2446..2462,1..3)
                         /regulatory_class="promoter"
                         /note="SP6 promoter"
         misc_feature    1
                         /note="SP6 transcription initiation site"
         misc_feature    4..90
                         /note="multiple cloning sites"
         regulatory      99..118
                         /regulatory_class="promoter"
                         /note="T7 promoter"
         misc_feature    101
                         /note="T7 transcription initiation site"
         gene            complement(1135..1995)
                         /gene="bla"
         CDS             complement(1135..1995)
                         /gene="bla"
                         /codon_start=1
                         /transl_table=11
                         /product="Beta-lactamase"
                         /protein_id="CAA46432.1"
                         /db_xref="GI:58240"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW"
    ORIGIN      
            1 gaactcgagc agctgaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
           61 gagctcgaat tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattaat
          121 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
          181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
          241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
          301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
          361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
          421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
          481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
          541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
          601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
          661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
          721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
          781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
          841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
          901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
          961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
         1021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
         1081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
         1141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
         1201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
         1261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
         1321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
         1381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
         1441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
         1501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
         1561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
         1621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
         1681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
         1741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
         1801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
         1861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
         1921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
         1981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
         2041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
         2101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
         2161 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
         2221 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
         2281 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
         2341 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
         2401 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
         2461 ta
    //
    
    

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