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  • PinPoint Xa-3

    價格:1350元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Promega
    載體名稱: PinPoint Xa-3
    質粒類型: 大腸桿菌表達載體;蛋白純化
    表達水平:
    啟動子: --
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 3333 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: Ampicillin
    篩選標記: --
    備注: --
    產品目錄號: V2061
    穩定性: 瞬表達
    組成型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    Pin point xa1載體圖譜
    Pin Point Xa3多克隆位點

    Pin Point Xa3載體特征

    載體簡介

    載體序列

    LOCUS       PinPoint Xa-3	3333 bp 	DNA	SYN
    DEFINITION  PinPoint Xa-3
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..3333
                         /organism="PinPoint Xa-3"
                         /mol_type="other DNA"
         misc_feature    1..386
                         /label="biotin_Pinpoint_Xa-1"
         CDS             1..468
                         /label="ORF frame 1"
                         /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                         GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                         KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
         misc_feature    complement(453..470)
                         /label="Sp6_primer"
                         /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                         GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                         KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
         misc_feature    670..692
                         /label="pGEX_3_primer"
                         /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                         GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                         KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
         promoter        851..879
                         /label="AmpR_promoter"
                         /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                         GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                         KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
         gene            921..1781
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                         GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                         KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
         CDS             921..1781
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         rep_origin      1936..2555
                         /label="pBR322_origin"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        2798..2816
                         /label="T7_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         misc_feature    2940..2959
                         /label="pBRrevBam_primer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        3211..3239
                         /label="tac_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         misc_feature    3251..3273
                         /label="M13_pUC_rev_primer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
       61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
      121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
      181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
      241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
      301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
      361 atcggggatc tcgagctcat cgaaggtcgc gaaaagcttc agctgggatc cggtaccgat
      421 atcagatctc ccggggcggc cgcgatctgg ttctatagtg tcacctaaat cgtatgtgta
      481 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt
      541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2341 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2761 cgattcatta atgcaggtta acctggctta tcgaaattaa tacgactcac tatagggaga
     2821 ccggcctcga gcagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     2881 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     2941 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     3001 atgcgtccgg cgtagaggat cgatccgggc ttatcgactg cacggtgcac caatgcttct
     3061 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa
     3121 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
     3181 ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg
     3241 tggaattgtg agcggataac aatttcacac aggaaacaga attcccagct tggctgcaga
     3301 accattccat tcgttgatcc gggagtaact cac
    //
    

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