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  • pTRE2hyg

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pTRE2hyg
    質粒類型: 哺乳動物細胞載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: --
    載體大小: 5337 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: --
    宿主細胞(系): --
    備注: --

    載體質粒圖譜和多克隆位點信息

    pTRE2hyg載體圖譜

    載體簡介

    載體序列

    LOCUS       pTRE2hyg                5337 bp ds-DNA     circular SYN 19-OCT-2017
    DEFINITION  Expression vector with a Tet-responsive promoter and a hygromycin 
                resistance marker.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pTRE2hyg
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 5337)
      AUTHORS   Clontech (TaKaRa)
      TITLE     Direct Submission
      JOURNAL   Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..5337
                         /organism="synthetic DNA construct"
                         /lab_host="Mammalian Cells"
                         /mol_type="other DNA"
         protein_bind    15..285
                         /bound_moiety="tetracycline repressor TetR"
                         /note="tetracycline response element"
                         /note="contains seven copies of the tetracycline operator 
                         tetO"
         promoter        318..356
                         /note="minimal CMV promoter"
                         /note="human cytomegalovirus (CMV) immediate early 
                         promoter"
         misc_feature    471..532
                         /note="MCS"
                         /note="multiple cloning site"
         intron          554..1126
                         /note="beta-globin intron"
                         /note="intron from rabbit beta-globin gene"
         polyA_site      1323..1378
                         /note="beta-globin poly(A) signal"
                         /note="rabbit beta-globin polyadenylation signal"
         rep_origin      complement(1947..2535)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(2706..3566)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(3567..3671)
                         /gene="bla"
                         /note="AmpR promoter"
         polyA_signal    3777..3858
                         /note="SV40 poly(A) signal"
                         /note="SV40 polyadenylation signal"
         CDS             complement(3975..5000)
                         /codon_start=1
                         /gene="aph(4)-Ia"
                         /product="aminoglycoside phosphotransferase from E. coli"
                         /note="HygR"
                         /note="confers resistance to hygromycin"
                         /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                         YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                         ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                         TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                         GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                         NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                         E"
         promoter        complement(5009..1)
                         /note="SV40 promoter"
                         /note="SV40 enhancer and early promoter"
         rep_origin      5023..5158
                         /note="SV40 ori"
                         /note="SV40 origin of replication"
    ORIGIN
            1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
           61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
          121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
          181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
          241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
          301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
          361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
          421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
          481 gtcagctgac gcgtgctagc gcggccgcat cgataagctt gtcgacgata tctctagagc
          541 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
          601 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
          661 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
          721 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
          781 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
          841 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
          901 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
          961 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
         1021 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
         1081 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
         1141 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
         1201 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
         1261 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
         1321 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
         1381 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
         1441 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
         1501 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
         1561 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
         1621 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
         1681 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg
         1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
         1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
         1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
         1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
         1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
         2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
         2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
         2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
         2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
         2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
         2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
         2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
         2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
         2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
         2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
         2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
         2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
         2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
         2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
         2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
         2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
         3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
         3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
         3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
         3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
         3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
         3301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
         3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
         3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
         3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
         3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
         3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
         3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
         3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca ctcgaggcag
         3781 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
         3841 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
         3901 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
         3961 gatcctctag aactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
         4021 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
         4081 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
         4141 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
         4201 agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag
         4261 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
         4321 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
         4381 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
         4441 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
         4501 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
         4561 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
         4621 agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt
         4681 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc
         4741 aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
         4801 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
         4861 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
         4921 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
         4981 gtgagttcag gctttttcat ggaagctttt tgcaaaagcc taggcctcca aaaaagcctc
         5041 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
         5101 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
         5161 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
         5221 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
         5281 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacaca
    //
    
    
    

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