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  • pSI

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Promega
    載體名稱: pSI載體
    質粒類型: 哺乳動物細胞表達載體
    啟動子: CMV
    表達水平:
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 3632 bp
    5' 測序引物:   SV40 pro-F: 5'-TATTTATGCAGAGGCCGAGG-3'
    3' 測序引物: EBV-R: 5'-GTGGTTTGTCCAAACTCATC-3'
    載體標簽: /
    載體抗性: 氨芐
    篩選標記: /
    備注:
    /
    產品目錄號: E1721
    穩定性: 穩表達/ 瞬表達
    組成型: 組成型 Constitutive
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pSI載體圖譜和多克隆位點

    載體簡介

    PCI和PSI 哺乳動物表達載體,旨在提升哺乳動物細胞中插入的克隆DNA基因的組成型表達。這兩個載體之間的差異在于增強子/啟動子區域,該區域能夠控制插入基因的表達水平。 PSI表達載體包含猿猴病毒40(SV40)增強子和早期啟動子區域(early promoter region),而PCI表達載體含有人巨細胞病毒(CMV)的主要及時早期基因增強子/啟動子區域(immediate-early gene enhancer/promoter region)。

    PSI和PCI載體可用于哺乳細胞的瞬時和穩定基因表達。對于哺乳細胞基因的穩表達,PSI或PCI載體必須共轉染一個可篩選的表達載體。

    載體序列

    LOCUS       pSI	3632 bp 	DNA     circular	SYN
    DEFINITION  pSI
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    FEATURES             Location/Qualifiers
         source          1..3632
                         /organism="pSI"
                         /mol_type="other DNA"
         misc_feature    complement(79..99)
                         /label="pBABE_3_primer"
         misc_feature    complement(85..300)
                         /label="SV40_enhancer"
         promoter        97..365
                         /label="SV40_promoter"
         rep_origin      264..341
                         /label="SV40_origin"
         misc_feature    326..345
                         /label="SV40pro_F_primer"
         misc_feature    483..615
                         /label="chim_int"
         promoter        660..678
                         /label="T7_promoter"
         terminator      complement(743..862)
                         /label="SV40_PA_terminator"
         misc_feature    complement(757..776)
                         /label="EBV_rev_primer"
         rep_origin      1065..1371
                         /label="f1_origin"
         misc_feature    1689..1711
                         /label="pGEX_3_primer"
         promoter        1870..1898
                         /label="AmpR_promoter"
         gene            1940..2800
                         /label="Ampicillin"
                         /gene="Ampicillin"
         CDS             1940..2800
                         /label="ORF frame 2"
         rep_origin      2955..3574
                         /label="pBR322_origin"
    ORIGIN
        1 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG
       61 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
      121 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
      181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
      241 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
      301 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
      361 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG
      421 ATTCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC
      481 AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA
      541 GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG
      601 CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT
      661 AATACGACTC ACTATAGGCT AGCCTCGAGA ATTCACGCGT GGTACCTCTA GAGTCGACCC
      721 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT
      781 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
      841 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
      901 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA
      961 ATCGATAAGG ATCCGGGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
     1021 CAGTTGCGCA GCCTGAATGG CGAATGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
     1081 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
     1141 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
     1201 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
     1261 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
     1321 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
     1381 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
     1441 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA
     1501 TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
     1561 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
     1621 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
     1681 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
     1741 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC
     1801 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT
     1861 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA
     1921 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT
     1981 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC
     2041 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT
     2101 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT
     2161 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA
     2221 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG
     2281 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA
     2341 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG
     2401 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA
     2461 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG
     2521 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT
     2581 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG
     2641 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC
     2701 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA
     2761 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC
     2821 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT
     2881 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC
     2941 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
     3001 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT
     3061 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT
     3121 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT
     3181 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG
     3241 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
     3301 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA
     3361 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG
     3421 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA
     3481 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG
     3541 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
     3601 CTGGCCTTTT GCTCACATGG CTCGACAGAT CT
    //
    
    

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