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  • pGL4.11[luc2P]

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pGL4.11載體基本信息

    出品公司: Promega
    載體名稱: pGL4.11 ; pGL4.11[luc2P]
    質粒類型: 信號通路報告載體;哺乳動物載體;螢火蟲熒光素酶報告載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子:
    載體大小: 4370 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: luc2P
    載體抗性: 氨芐青霉素
    篩選標記: 潮霉素(Hygromycin)
    克隆菌株: TOP10等常規菌株
    宿主細胞(系): HEK293等
    備注: pGL4.11[luc2P]載體是信號通路報告載體,無啟動子,含螢火蟲熒光 素酶報告基因luc2P
    產品目錄號: E666A
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    pGL4.11載體質粒圖譜和多克隆位點信息

    pGL4.11載體圖譜



    pGL4.11 多克隆位點

    pGL4.11 載體特征

    pGL4.11載體簡介

    The pGL4.11[luc2P] Vector encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.11[luc2P] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
    

    pGL4.11載體序列

    LOCUS       AY738223                4370 bp    DNA     circular SYN 08-NOV-2004
    DEFINITION  Firefly luciferase reporter vector pGL4.11[luc2P], complete
                sequence.
    ACCESSION   AY738223
    VERSION     AY738223.1  GI:55535621
    KEYWORDS    .
    SOURCE      Firefly luciferase reporter vector pGL4.11[luc2P]
      ORGANISM  Firefly luciferase reporter vector pGL4.11[luc2P]
                other sequences; artificial sequences; vectors.
    REFERENCE   1  (bases 1 to 4370)
      AUTHORS   Almond,B., Chauvin,F. and Browning,J.
      TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
                Corporation
      JOURNAL   Unpublished
    REFERENCE   2  (bases 1 to 4370)
      AUTHORS   Almond,B., Chauvin,F. and Browning,J.
      TITLE     Direct Submission
      JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
                Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
    FEATURES             Location/Qualifiers
         source          1..4370
                         /organism="Firefly luciferase reporter vector
                         pGL4.11[luc2P]"
                         /mol_type="other DNA"
                         /db_xref="taxon:296660"
         misc_feature    1..70
                         /note="multiple cloning site region"
         CDS             100..1875
                         /codon_start=1
                         /transl_table=11
                         /product="luciferase luc2P"
                         /protein_id="AAV52871.1"
                         /db_xref="GI:55535622"
                         /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                         AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                         APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                         FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                         FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                         LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                         GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                         RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                         PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                         AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
                         LPMSCAQESGMDRHPAACASARINV"
         misc_feature    1915..2136
                         /note="SV40 late poly(A) region"
         primer_bind     2204..2223
                         /note="reporter vector primer 4 (RVPrimer4)"
         rep_origin      2461
                         /note="ColE1-derived"
         gene            complement(3252..4112)
                         /gene="ampR"
         CDS             complement(3252..4112)
                         /gene="ampR"
                         /note="AmpR"
                         /codon_start=1
                         /transl_table=11
                         /product="synthetic beta-lactamase"
                         /protein_id="AAV52872.1"
                         /db_xref="GI:55535623"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW"
         regulatory      4217..4370
                         /regulatory_class="polyA_signal_sequence"
                         /note="synthetic; transcriptional pause site"
         primer_bind     complement(4217..4370)
                         /note="reporter vector primer 3 (RVPrimer3)"
    ORIGIN      
            1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
           61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
          121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
          181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
          241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
          301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
          361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
          421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
          481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
          541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
          601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
          661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
          721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
          781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
          841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
          901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
          961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
         1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
         1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
         1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
         1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
         1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
         1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
         1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
         1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
         1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
         1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
         1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
         1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
         1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
         1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
         1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
         1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
         1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
         2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
         2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
         2161 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
         2221 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
         2281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
         2341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
         2401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
         2461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
         2521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
         2581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
         2641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
         2701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
         2761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
         2821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
         2881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
         2941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
         3001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
         3061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
         3121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
         3181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
         3241 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
         3301 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
         3361 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
         3421 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
         3481 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
         3541 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
         3601 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
         3661 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
         3721 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
         3781 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
         3841 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
         3901 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
         3961 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
         4021 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
         4081 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
         4141 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
         4201 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
         4261 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
         4321 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
    //
    

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