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  • pCMV-Tag 4A

    價格:1250元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Stratagene
    載體名稱: pCMV-Tag 4A
    質粒類型: 哺乳動物表達載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 多克隆位點;限制性內切酶
    啟動子: CMV
    載體大小: 4319 bp
    5' 測序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: T7
    載體標簽: FLAG tag (C-末端)
    載體抗性: 卡那霉素
    篩選標記: Neomycin
    克隆菌株: XL1-Blue
    宿主細胞(系): --
    備注: FLAG標簽含8個氨基酸殘基(DYKDDDDK);
    pCMV-Tag 4 A,B,C的差別僅在于讀碼框(閱讀框)處。
    產品目錄號: 211174
    穩定性: 瞬表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pCMV-Tag 4A載體圖譜



    pCMV-Tag 4A 多克隆位點

    pCMV-Tag 4A 載體特征

    載體簡介

    ADDITIONAL MATERIALS REQUIRED
    T4 DNA ligase
    Taq DNA polymerase
    Taq DNA polymerase buffer
    TE buffer
    CIAP
    Falcon 2059 polypropylene tubes (15-ml)
    Water baths (37°C and 42°C)
    
    XL1-Blue Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZΔM15 Tn10 (Tetr)].
    The XL1-Blue cells supplied with the pCMV-Tag vectors are not competent cells. Refer to Hanahan (1983) for a protocol for producing competent cells.
    

    載體序列

    LOCUS       pCMV-Tag 4A	4319 bp 	DNA	SYN
    DEFINITION  pCMV-Tag 4A
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..4319
                         /organism="pCMV-Tag 4A"
                         /mol_type="other DNA"
         promoter        16..568
                         /label="CMV_immearly_promoter"
         misc_feature    71..358
                         /label="CAG_enhancer"
         misc_feature    525..545
                         /label="CMV_fwd_primer"
         promoter        526..595
                         /label="CMV_promoter"
         promoter        620..639
                         /label="T3_promoter"
         misc_feature    complement(726..742)
                         /label="pBluescriptKS_primer"
         promoter        complement(821..839)
                         /label="T7_promoter"
         misc_feature    896..955
                         /label="SV40_3_splice"
         terminator      1006..1234
                         /label="SV40_PA_terminator"
         misc_feature    1204..1223
                         /label="EBV_rev_primer"
         rep_origin      complement(1373..1679)
                         /label="f1_origin"
         promoter        1758..1786
                         /label="AmpR_promoter"
         misc_feature    complement(1852..1872)
                         /label="pBABE_3_primer"
         misc_feature    complement(1858..2073)
                         /label="SV40_enhancer"
         promoter        1870..2138
                         /label="SV40_promoter"
         rep_origin      2037..2114
                         /label="SV40_origin"
         misc_feature    2099..2118
                         /label="SV40pro_F_primer"
         CDS             2221..3015
                         /label="ORF frame 1"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                         PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                         LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                         EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                         QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
         gene            2224..3012
                         /label="NeoR/KanR (variant)"
                         /gene="NeoR/KanR (variant)"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                         PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                         LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                         EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                         QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
         CDS             complement(2530..3066)
                         /label="ORF frame 3"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         terminator      3190..3459
                         /label="TK_PA_terminator"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         rep_origin      3607..4226
                         /label="pBR322_origin"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
    ORIGIN
        1 ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA
       61 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG
      121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG
      181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA
      241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC
      301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC
      361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC
      421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA
      481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG
      541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA
      601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTCCACC
      661 GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC
      721 TTATCGATAC CGTCGACCTC GAGGATTACA AGGATGACGA CGATAAGTAG GGCCCGGTAC
      781 CTTAATTAAT TAAGGTACCA GGTAAGTGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC
      841 AATTCACTCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GAGATCCAAT
      901 TTTTAAGTGT ATAATGTGTT AAACTACTGA TTCTAATTGT TTGTGTATTT TAGATTCACA
      961 GTCCCAAGGC TCATTTCAGG CCCCTCAGTC CTCACAGTCT GTTCATGATC ATAATCAGCC
     1021 ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC
     1081 TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT
     1141 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
     1201 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAACGCGT AAATTGTAAG CGTTAATATT
     1261 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA
     1321 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA
     1381 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC
     1441 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG
     1501 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG
     1561 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG
     1621 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG
     1681 CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
     1741 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
     1801 CTTCAATAAT ATTGAAAAAG GAAGAATCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT
     1861 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC
     1921 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA
     1981 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC
     2041 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA
     2101 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT
     2161 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC
     2221 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
     2281 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
     2341 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
     2401 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
     2461 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
     2521 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
     2581 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
     2641 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
     2701 GAACATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC
     2761 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
     2821 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC
     2881 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAACTTGGCG GCGAATGGGC TGACCGCTTC
     2941 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT
     3001 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC
     3061 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG
     3121 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG
     3181 CCCACCCTAG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG
     3241 CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGTGT TGGGTCGTTT GTTCATAAAC
     3301 GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC
     3361 AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG
     3421 GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC CTCAGGTTAC TCATATATAC
     3481 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG
     3541 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG
     3601 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC
     3661 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC
     3721 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT
     3781 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC
     3841 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT
     3901 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC
     3961 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG
     4021 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG
     4081 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG
     4141 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA
     4201 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT
     4261 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCC
    
    //
    
    

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