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  • pCMV-SPORT6.1

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen
    載體名稱: pCMV-SPORT6.1
    質粒類型: 哺乳動物表達載體;cDNA克隆載體;I.M.A.G.E.載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點;Gateway載體
    啟動子: CMV
    載體大小: 4177 bp
    5' 測序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
    3' 測序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
    載體標簽: --
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: DH5α
    宿主細胞(系): 哺乳動物細胞
    備注:
    pCMV-SPORT6.1有多個限制性酶切位點,又可用于Gateway克隆,用于cDNA文庫的構建和表達。
     
    產品目錄號: --
    穩定性: 瞬表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pCMV-SPORT6.1載體圖譜
    pCMV-SPORT6.1多克隆位點

    載體簡介

    載體序列

    LOCUS       Exported                4177 bp ds-DNA     circular SYN 12-AUG-2016
    DEFINITION  Gateway(R) library vector for cloning and transient mammalian cell 
                expression of cDNAs. See pCMV SPORT6 for an older version of this 
                vector.
    KEYWORDS    pCMV SPORT6.1
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 4177)
      AUTHORS   Invitrogen (Life Technologies)
    COMMENT     The cDNA is inserted between bases 781-782, destroying an EcoRV site
                present in the original vector.
    FEATURES             Location/Qualifiers
         source          1..4177
                         /organism="synthetic DNA construct"
                         /lab_host="Mammalian Cells"
                         /mol_type="other DNA"
         polyA_signal    125..259
                         /note="SV40 poly(A) signal"
                         /note="SV40 polyadenylation signal"
         primer_bind     664..680
                         /note="M13 fwd"
                         /note="common sequencing primer, one of multiple similar 
                         variants"
         promoter        691..709
                         /note="T7 promoter"
                         /note="promoter for bacteriophage T7 RNA polymerase"
         protein_bind    711..735
                         /gene="mutant version of attB"
                         /bound_moiety="BP Clonase(TM)"
                         /note="attB2"
                         /note="recombination site for the Gateway(R) BP reaction"
         misc_feature    736..806
                         /note="MCS"
         protein_bind    complement(807..831)
                         /gene="mutant version of attB"
                         /bound_moiety="BP Clonase(TM)"
                         /note="attB1"
                         /note="recombination site for the Gateway(R) BP reaction"
         promoter        complement(832..850)
                         /note="SP6 promoter"
                         /note="promoter for bacteriophage SP6 RNA polymerase"
         primer_bind     complement(861..877)
                         /note="M13 rev"
                         /note="common sequencing primer, one of multiple similar 
                         variants"
         promoter        complement(1003..1206)
                         /note="CMV promoter"
                         /note="human cytomegalovirus (CMV) immediate early 
                         promoter"
         enhancer        1207..1510
                         /note="CMV enhancer"
                         /note="human cytomegalovirus immediate early enhancer"
         rep_origin      complement(1970..2558)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(2729..3589)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(3590..3694)
                         /gene="bla"
                         /note="AmpR promoter"
         rep_origin      complement(3721..4176)
                         /direction=LEFT
                         /note="f1 ori"
                         /note="f1 bacteriophage origin of replication; arrow 
                         indicates direction of (+) strand synthesis"
    ORIGIN
            1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
           61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
          121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
          181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
          241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
          301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
          361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
          421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
          481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
          541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
          601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac
          661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactttgt
          721 acaagaaagc tgggtacgcg taagcttggg cccctcgagg gatactctag agcggccgcc
          781 catcccggga attccggacc ggtaccagcc tgcttttttg tacaaacttg ttctatagtg
          841 tcacctaaat aggcctaatg gtcatagctg tttcctgtgt gaaattgtta tccgctccgc
          901 ggcctaggct agagtccgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
          961 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac
         1021 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
         1081 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
         1141 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
         1201 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
         1261 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
         1321 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
         1381 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
         1441 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
         1501 ttatgtaacg acatgcatct aatgagtgaa agggcctcgt actacgccta tttttatagg
         1561 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
         1621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
         1681 aataaccctg ataaatgctt caataatatt gaaaaacgcg cgaattgcaa gctctgcatt
         1741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
         1801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
         1861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
         1921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
         1981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
         2041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
         2101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
         2161 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
         2221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
         2281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
         2341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
         2401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
         2461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
         2521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
         2581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
         2641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
         2701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
         2761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
         2821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
         2881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
         2941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
         3001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
         3061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
         3121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
         3181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
         3241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
         3301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
         3361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
         3421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
         3481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
         3541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
         3601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
         3661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
         3721 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
         3781 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
         3841 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
         3901 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
         3961 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
         4021 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
         4081 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
         4141 cacccgccgc gcttaatgcg ccgctacagg gcgcgtc
    //
    
    

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