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  • pCMV-Gaussia-Dura-Luc

    價格:3800元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: INVITROGEN
    載體名稱: pCMV-Gaussia-Dura-Luc ; pCMV-Gaussia-Dura Luc
    質粒類型: 哺乳動物載體;分泌型熒光素酶報告載體
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: CMV
    載體大小: 5482 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: Gaussia-Dura 分泌型熒光素酶
    載體抗性: 氨芐青霉素
    篩選標記: 嘌呤霉素(Puromycin)
    克隆菌株: TOP10等常規菌株
    宿主細胞(系): 常規哺乳動物細胞
    備注: pCMV-Gaussia-Dura-Luc載體表達分泌型的熒光素酶,可直接在培養基中進行檢測。
    產品目錄號: 16191
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pCMV-Gaussia-Dura-Luc載體圖譜



    pCMV-Gaussia-Dura-Luc載體特征

    載體簡介

    pCMV-Gaussia-Dura Luc 載體簡介
    
    The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the strong Cytomegalovirus (CMV) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.
    
    載體特征:
    
     Strong (CMV) constitutive promoter for co-transfection and normalization
     Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
     Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
     Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
     Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
     High-copy pUC bacterial DNA replication origin
    
    Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
    

    載體序列

    LOCUS       Exported                5482 bp ds-DNA     circular SYN 24-JUL-2016
    DEFINITION  Control vector for constitutive high-level expression of secreted 
                Gaussia-Dura luciferase under control of the CMV promoter.
    ACCESSION   .
    VERSION     .
    KEYWORDS    pCMV-Gaussia-Dura Luc
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 5482)
      AUTHORS   Thermo Scientific (Pierce)
      TITLE     Direct Submission
      JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
                http://www.snapgene.com
    FEATURES             Location/Qualifiers
         source          1..5482
                         /organism="synthetic DNA construct"
                         /lab_host="Mammalian Cells"
                         /mol_type="other DNA"
         enhancer        63..366
                         /note="CMV enhancer"
                         /note="human cytomegalovirus immediate early enhancer"
         promoter        367..570
                         /note="CMV promoter"
                         /note="human cytomegalovirus (CMV) immediate early 
                         promoter"
         promoter        615..633
                         /note="T7 promoter"
                         /note="promoter for bacteriophage T7 RNA polymerase"
         CDS             646..1203
                         /codon_start=1
                         /product="enhanced variant of secreted Gaussia luciferase"
                         /note="Gaussia-Dura Luc"
                         /note="human codon-optimized"
                         /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                         KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                         QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                         RCATFASKIQGQVDKIKGAGGD"
         polyA_signal    1226..1337
                         /note="BGH pA"
                         /note="bovine growth hormone polyadenylation signal"
         promoter        1460..1789
                         /note="SV40 promoter"
                         /note="SV40 enhancer and early promoter"
         rep_origin      1640..1775
                         /note="SV40 ori"
                         /note="SV40 origin of replication"
         promoter        1837..1884
                         /note="EM7 promoter"
                         /note="synthetic bacterial promoter "
         CDS             1903..2502
                         /codon_start=1
                         /gene="pac from Streptomyces alboniger"
                         /product="puromycin N-acetyltransferase"
                         /note="Puromycin"
                         /note="confers resistance to puromycin"
                         /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                         VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                         AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                         APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
         polyA_signal    2632..2753
                         /note="SV40 pA"
                         /note="SV40 polyadenylation signal"
         CDS             complement(2806..3666)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="Ampicillin"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         rep_origin      3930..4518
                         /direction=RIGHT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         protein_bind    5260..5276
                         /bound_moiety="lac repressor encoded by lacI"
                         /note="lac operator"
                         /note="The lac repressor binds to the lac operator to 
                         inhibit transcription in E. coli. This inhibition can be 
                         relieved by adding lactose or 
                         isopropyl-beta-D-thiogalactopyranoside (IPTG)."
         misc_feature    5391..5482
                         /note="TPS"
                         /note="RNA polymerase II transcriptional pause signal from 
                         the human alpha-2 globin gene"
    ORIGIN
            1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
           61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
          121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
          181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
          241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
          301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
          361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
          421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
          481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
          541 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
          601 tggcttatcg aaattaatac gactcactat aggggatccg ccaccatggg agtcaaagtt
          661 ctgtttgccc tgatctgcat cgctgtggcc gaggccaagc ccaccgagaa caacgaagac
          721 ttcaacatcg tggccgtggc cagcaacttc gcgaccacgg atctcgatgc tgaccgcggg
          781 aagttgcccg gcaagaagct gccgctggag gtgctcaaag agttggaagc caatgcccgg
          841 aaagctggct gcaccagggg ctgtctgatc tgcctgtccc acatcaagtg cacgcccaag
          901 atgaagaagt tcatcccagg acgctgccac acctacgaag gcgacaaaga gtccgcacag
          961 ggcggcatag gcgaggcgat cgtcgacatt cctgagattc ctgggttcaa ggacttggag
         1021 cccttggagc agttcatcgc acaggtcgat ctgtgtgtgg actgcacaac tggctgcctc
         1081 aaagggcttg ccaacgtgca gtgttctgac ctgctcaaga agtggctgcc gcaacgctgt
         1141 gcgacctttg ccagcaagat ccagggccag gtggacaaga tcaagggggc cggtggtgac
         1201 taagcggccg caaaatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
         1261 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
         1321 aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt gatttataag
         1381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
         1441 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
         1501 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
         1561 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
         1621 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
         1681 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
         1741 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
         1801 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
         1861 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga gtacaagccc
         1921 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
         1981 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
         2041 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
         2101 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
         2161 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
         2221 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
         2281 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
         2341 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc cgcgccccgc
         2401 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
         2461 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacacgtgct acgagatttc
         2521 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
         2581 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
         2641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
         2701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
         2761 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcattacc aatgcttaat
         2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
         2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat
         2941 accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag
         3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
         3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc
         3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
         3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
         3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
         3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
         3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
         3421 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
         3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
         3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
         3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
         3661 actcatattc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
         3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa
         3781 ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct cataacaccc
         3841 cttgctcatg accaaaatcc cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca
         3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
         3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
         4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
         4081 ctagtgtagc cgtagttagc ccaccacttc aagaactctg tagcaccgcc tacatacctc
         4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
         4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
         4261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
         4321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
         4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
         4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
         4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
         4561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
         4621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
         4681 gtgagcgagg aagcggaagg cgagagtagg gaactgccag gcatcaaact aagcagaagg
         4741 cccctgacgg atggcctttt tgcgtttcta caaactcttt ctgtgttgta aaacgacggc
         4801 cagtcttaag ctcgggcccc ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
         4861 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
         4921 tcagaatatt taagggcgcc tgtcactttg cttgatatat gagaattatt taaccttata
         4981 aatgagaaaa aagcaacgca ctttaaataa gatacgttgc tttttcgatt gatgaacacc
         5041 tataattaaa ctattcatct attatttatg attttttgta tatacaatat ttctagtttg
         5101 ttaaagagaa ttaagaaaat aaatctcgaa aataataaag ggaaaatcag tttttgatat
         5161 caaaattata catgtcaacg ataatacaaa atataataca aactataaga tgttatcagt
         5221 atttattatc atttagaata aattttgtgt cgcccttaat tgtgagcgga taacaattac
         5281 gagcttcatg cacagtggcg ttgacattga ttattgacta gcatgttctt tcctgcgtta
         5341 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat aacatacgct
         5401 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
         5461 gcaggtgcca gaacatttct ct
    //
    
    
    

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